Yes, siRNAs can be ordered in plates.

For plate order inquiries, Contact us.

Duplex siRNA: A sense and antisense strand with 2-nt 3′ overhangs. Standard for RISC loading and mRNA cleavage.

Triplex siRNA: Includes a third RNA strand using Hoogsteen interactions. Enhances specificity or introduces novel regulatory control.

No. siRNAs are delivered as duplexes and do not require annealing.

Most siRNAs are 21‑mers with 2‑nt 3′ overhangs (UU or dT‑dT). Dicer‑substrates (25–27 nt) can improve potency for some targets.

21mer siRNAs: Mimic Dicer products, bypass Dicer, and enter RISC directly.

27mer Dicer-substrate siRNAs: Require Dicer processing, improving RISC loading and gene knockdown efficiency.

Begin with 10–50 nM and optimize depending on the specific cell line and experiment.

27mer Dicer-substrate siRNAs: Require Dicer processing, improving RISC loading and gene knockdown efficiency.

Yes, though in vivo applications often require delivery vehicles such as lipid nanoparticles.

Yes—annealed duplex is the default. Single strands can be supplied for in‑house annealing if preferred.

We recommend 2′‑OMe in the guide seed, strategic sense‑strand modification/deactivation, and optional pooling (3–4 siRNAs). We can advise during design.

2′‑modifications (especially 2′‑OMe) help reduce TLR7/8 activation; avoiding certain sequence motifs also helps. Share your model system and we’ll tailor accordingly.

1. Lipid-Based Transfection (Most Common)
- High efficiency, easy protocol
- Best for most adherent and suspension cells
- Limitations: Can be toxic at high doses

2. Electroporation
- Ideal for hard-to-transfect cells like neurons and T cells
- Limitations: May cause cell death without optimization

3. Nucleofection
- Example: Lonza Nucleofector
- High efficiency for stem cells, neurons, and immune cells
- Limitations: Expensive and protocol-specific

4. Polymer-Based Reagents
- Example: PEI, JetPEI
- Cost-effective for scalable transfection
- Limitations: Lower efficiency, variable results

5. Viral Vectors (for shRNA, not siRNA)
- Suitable for long-term knockdown, not direct siRNA delivery.

Store lyophilized siRNA at 4 °C (short‑term) or −20 °C (long‑term). In solution, use RNase‑free buffer, aliquot, and keep at −20 °C to minimize freeze‑thaw cycles.

Low Knockdown Efficiency: Verify sequence, concentration, and delivery method.

Precipitation: Ensure correct solvent use and avoid overheating.

Degradation: Use RNase-free techniques and storage solutions.