Label Oligos Oligo Material Un-Annealed Material Non-Denaturing Polyacrylamide Gel

A method to check annealed oligo/ How to calculate quantity and QC the product

*Run an aliquot of the annealed product alongside the un-annealed material on a non-denaturing polyacrylamide gel

*End label oligos to visualize the material or back-shadow the gel with a UV light

*In this manner only a tiny fraction of the oligo material is used in the analysis

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