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Acetylated Peptide Synthesis (Peptide Acetylation)

Site-defined homogeneous acetylated peptides with defined modification site(s) and stoichiometry. Use PTM panels to map binding specificity, model enzyme “on/off” states, and create reliable assay controls.

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Overview Acetylation options Applications Design tips QC & handling FAQ Reading Contact

Overview

Peptide acetylation is a common post-translational modification (PTM) that regulates stability, localization, and molecular recognition. In peptides, acetylation most often occurs at the N-terminus or on the ε-amino group of lysine residues. These defined acetylation states are widely used in epigenetics, chromatin biology, and PTM-dependent binding studies.

Bio-Synthesis provides acetylated peptide synthesis with site-defined N-terminal acetylation and lysine-acetylated peptides (ε-acetyllysine) delivered as homogeneous PTM peptides with controlled stoichiometry, purification, LC–MS/HPLC QC, and COA for epigenetics, binding assays, and enzyme-state modeling.

Why acetylated peptides?
  • Neutralize amine charge to modulate binding and conformation
  • Model biologically relevant acetylation states (native PTMs)
  • Enable reader-domain specificity mapping (e.g., bromodomains)
  • Provide defined controls for enzyme assays (HATs/HDACs)
Common applications
  • Histone PTM panels and chromatin biology
  • Protein–protein interaction and peptide–protein binding assays
  • Enzyme on/off state modeling (acetyltransferases, deacetylases)
  • Assay calibration, benchmarking, and negative controls

Looking for other PTM categories? Explore our post-translational peptide modifications hub and related PTM services such as phosphorylated peptides and methylated peptides.

Acetylation types we synthesize (site-defined)

N-terminal acetylated peptides

N-terminal acetylation is among the most common PTMs in eukaryotic proteins and can influence stability and recognition. Synthetic N-acetyl peptides are widely used to mimic native maturation states and to cap N-termini for binding studies.

  • Nα-acetylation on the N-terminus (Ac–peptide)
  • Optional C-terminus format: acid or amide
  • Matched unmodified control recommended
Lysine-acetylated peptides (ε-acetylation)

Lysine acetylation neutralizes the lysine side-chain charge and is central to epigenetic regulation and reader-domain recognition. We support single-site and multi-site lysine acetylation for histone PTM panels and specificity mapping.

  • Site-specific ε-N-acetyllysine at defined residue positions
  • Single-site variants and combinatorial PTM panels
  • Compatible with additional PTMs for crosstalk studies (e.g., methylation)
Quick chooser (60 seconds)
Use “acetylated PTM peptide” when you need…
  • Biologically faithful acetylation states (native PTM)
  • Reader-domain binding specificity (bromodomains)
  • Enzyme-state modeling (HATs/HDACs on/off)
  • Defined stoichiometry and reproducible controls
Use “conjugated / functionalized peptide” when you need…
  • Detection labels (fluorescence, FRET)
  • Capture tags (biotin) or immobilization
  • Handles for assembly (azide/alkyne/DBCO click)
  • Solubility/PK tuning (PEG, lipid payloads)

Not sure which to choose? Tell us your assay and whether you’re mimicking an in vivo state or adding a workflow handle—we’ll recommend the right format.

Applications

Epigenetics & chromatin

Histone acetylation peptides and PTM panels for chromatin biology, transcriptional regulation, and crosstalk mapping.

Reader-domain binding

Define bromodomain and other acetyl-lysine reader specificity using site-defined acetylation states and matched controls.

Enzyme assays

Substrates and controls for acetyltransferases (HATs) and deacetylases (HDACs), including multi-site variants.

Why synthetic acetylated peptides outperform enzymatic mixtures
  • Site-defined acetylation and homogeneous composition
  • Controlled stoichiometry (mono- vs multi-acetylation)
  • Matched controls (unmodified and site-variant panels)
  • Ready for quantitative workflows (including optional isotope labeling)

Design tips

Include these in your request
  • Acetylation type: N-terminal vs lysine
  • Exact residue position(s) and desired PTM state(s)
  • Terminal format (C-terminal acid vs amide)
  • Use case (binding, enzyme assay, LC–MS/MS)
Best-practice panels
  • Acetylated vs unmodified peptide pairs
  • Single-site variants to confirm positional specificity
  • Multi-PTM crosstalk sets (acetylation + methylation)
  • Negative controls (site-null or scrambled)

For chromatin workflows, consider ordering a small histone acetylation panel (multiple sites and states) rather than a single peptide to accelerate specificity mapping.

QC & handling

Quality control deliverables
  • Analytical HPLC chromatogram
  • LC–MS confirmation of expected acetylation mass shift
  • Certificate of Analysis (COA)
  • PTM panel QC notes (on request)
Handling & stability guidance
  • Storage and aliquoting guidance to minimize freeze–thaw
  • Buffer and pH recommendations for your assay
  • Notes for multi-PTM peptides and long incubations
  • Assay-specific handling (binding vs LC–MS workflows)

Tip: Share assay conditions early (buffer, pH, incubation time, detection method) so we can recommend formats that preserve PTM integrity and reduce redesign cycles.

FAQ

Are acetylated peptides synthetic?

Yes. Research-grade acetylated peptides are typically chemically synthesized so acetylation is installed at defined residue positions and stoichiometry. Synthetic acetylated peptides avoid heterogeneity and are preferred for mechanistic studies, quantitative LC–MS workflows, and assay controls.

What’s the difference between N-terminal acetylation and lysine acetylation?

N-terminal acetylation modifies the peptide N-terminus, often affecting stability or recognition. Lysine acetylation modifies the ε-amino group of lysine side chains and is central to chromatin biology and reader-domain interactions.

Can you synthesize multi-acetylated peptides and panels?

Yes. We support single-site and multi-site acetylated peptides, including PTM panels with matched unmodified and site-variant controls.

Can acetylation be combined with other PTMs?

Yes. Acetylation is commonly combined with methylation and other PTMs to study crosstalk. Tell us your study design and we’ll recommend a panel layout.

More FAQ'sAll FAQ's

Contact & quote request

Send your sequence(s), acetylation site(s), and specifications. We’ll respond with a synthesis plan and QC options aligned to your assay or LC–MS workflow.

Request a quote for acetylated peptide synthesis
  • Sequence (N→C) + acetylation site(s) and type (N-terminal vs ε-Lys)
  • Terminal format (acid vs amide; any additional caps/handles)
  • Purity/quantity targets
  • Use case (binding, enzyme assay, LC–MS/MS)
Request a Quote Review acetylation options

What happens next: Our technical team reviews requests and responds with feasibility notes, recommended controls/panels, a QC plan, and pricing.

Fast quote checklist
  • Peptide sequence(s) + terminal state (free vs N-acetyl; acid vs amide)
  • Exact acetylation site(s) (N-terminus and/or Lys position numbers)
  • Single-site vs multi-site acetylation and any panel needs
  • Quantity (mg) + purity target + intended use
  • Any additional PTMs (e.g., methylation) for crosstalk studies

If you’re unsure whether you need N-terminal acetylation, ε-acetyllysine, or both, share your assay target (bromodomain/antibody/enzyme) and we’ll recommend a clean panel design.

Talk to a chemist Request a quote

Recommended reading

Background on acetylation biology, histone PTMs, and acetyl-lysine recognition.

Want a tailored reading list for your acetylation target and assay type? Share your application and we’ll refine it.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

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