Design oligos with on-demand release using photocleavable, reductive, acid-labile, enzyme-cleavable, and self-immolative linkers. Optimized for research to GMP-like supply with UPLC/HPLC & LC-MS QC.
Bio‑Synthesis provides end‑to‑end solutions for controlled release (cleavable) linkers on DNA and RNA oligonucleotides. We engineer light‑activated, redox‑responsive, acid‑labile, and enzyme‑cleavable designs to enable on‑demand cargo release, higher signal‑to‑noise, and tissue‑ or pathway‑specific activation.
Our capabilities span photocleavable linkers for oligonucleotides (o‑nitrobenzyl/DMNB/NVOC and blue‑light coumarins), disulfide linkers for siRNA/ASO delivery, hydrazone and other acid‑labile linkers for endosomal release, and enzyme‑cleavable peptide linkers (Val‑Cit‑PABC, MMP motifs) with self‑immolative spacers for traceless payload liberation. From screening µmol to multi‑gram campaigns, we provide plate formats, custom conjugations (peptides, lipids, dyes, biotin), and matched analytics.
Our cleavable linker portfolio includes multiple strategies in a unified design framework:
This single framework simplifies design choices while improving clarity and SEO relevance for cleavable linker oligonucleotides.
Photocleavable for precise optical control; disulfide for intracellular reductive release; hydrazone for endosomal pH; enzyme‑cleavable peptides for tissue specificity; PABC for traceless self‑immolation.
Prefer termini/loops and add HEG/PEG spacers to preserve Tm and activity; confirm by melting curves.
Yes—tubes or 96‑well plates with barcoding, harmonized purification, and matched QC across wells.
The choice depends on your application: photocleavable linkers (o-nitrobenzyl, DMNB, coumarins) are best for optical control; disulfide linkers for intracellular reductive environments (siRNA/ASO delivery); acid-labile hydrazone linkers for endosomal release; enzyme-cleavable peptide linkers (Val-Cit-PABC, MMP) for tissue specificity; and self-immolative spacers for traceless activation.
Bulky linkers can shift melting temperature (Tm). We recommend terminal placement or loop regions, plus PEG/HEG spacers to minimize steric effects. For therapeutic designs (siRNA, ASO), validation with melting curves and LC-MS is recommended.
Yes. Bio-Synthesis offers ISO 9001/13485-aligned workflows, RUO → GMP-like documentation, and complete QC packages including UPLC/HPLC, LC-MS, cleavage kinetics, and serum stability studies.
Applications include siRNA and antisense oligonucleotide delivery, antibody-oligo conjugates (AOC/ADC), affinity capture and controlled photorelease, optogenetics, spatial transcriptomics, and DNA repair and chemical ligation studies.
Yes. We frequently combine cleavable linkers with lipid conjugates (cholesterol, GalNAc, DHA), peptides, fluorescent dyes, or affinity tags (biotin). This enables targeted delivery with on-demand activation.
Yes. We offer tube and 96-well plate formats, barcoded for automation, suitable for HTS assays and drug discovery campaigns.
Please avoid confidential details; we can arrange an NDA if needed.
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