Login / Register Order My Items
Contact Us
800.227.0627
Contact Us Quote Order Login / Register

Nanomaterial Conjugation Services — Gold, Silica, Magnetic & Polymeric Nanoparticles

Engineered nanomaterial conjugates with controlled size, surface charge, and functional loading. We develop stable, assay-ready nanoparticles using thiol–gold, EDC/NHS, maleimide, click, and biotin–streptavidin chemistries for delivery, imaging, and diagnostics.

Start a Project Overview Nanomaterial Platforms
ISO 9001:2015 / ISO 13485:2016
Gold · Silica · SPION · PLGA
Drug Delivery & Diagnostics
High Loading · Low Background
Confidential & IP-Protected
Overview Nanomaterial Platforms Conjugation Chemistries Applications Technical FAQ Contact Recommended Reading

Overview

Our nanomaterial conjugation services immobilize or encapsulate antibodies, proteins, peptides, oligonucleotides, and small-molecule drugs on engineered nanoparticles. We work with gold, silica, magnetic, polymeric, and carbon-based nanomaterials to build high-performing conjugates for research, diagnostics, and delivery.

Each project is designed around three constraints: nanoparticle physics (size, charge, PDI), surface chemistry (linker, density, orientation), and biological performance (activity, toxicity, nonspecific binding). We tune these variables together, then validate performance under realistic buffers and sample matrices.

Nanocarrier
Gold · Silica · Magnetic · Polymeric · Carbon
Linker & Surface Chemistry
Thiol–Gold · EDC/NHS · Maleimide · Click · PEG
Payload / Ligand
Antibodies · Oligos · Drugs · Peptides

Nanomaterial conjugation can be combined with our drug delivery and biosensing programs or integrated into existing kit and platform technologies.

Application-Tuned Nanocarriers

Gold for optical assays, silica and polymers for drug loading, SPIONs for magnetic and MRI-based work, and carbon materials for electrochemical sensing.

Controlled Loading & Stability

Defined ligand-to-particle ratios, PEG architectures, and blocking strategies to maximize activity while maintaining colloidal stability.

Documentation & Scale

Lot-tracked production with SOPs, QC data packages, and tech transfer support for kit, OEM, or translational programs.

Nanomaterial Platforms

Gold Nanoparticles (AuNPs)
  • Sizes from <5 nm to >100 nm with spherical or specialized geometries.
  • Citrate, amine, PEG, and streptavidin-modified surfaces.
  • Ideal for colorimetric assays, plasmonic sensors, lateral-flow tests, and imaging probes.
Silica & Mesoporous Silica Nanoparticles
  • Solid and mesoporous formats with tunable pore size and surface area.
  • Silanization with amine, thiol, or PEG groups for covalent coupling.
  • Suitable for drug loading, oligo capture, and multifunctional theranostic systems.
Polymeric Nanoparticles
  • PLGA, PEG-PLGA, and chitosan-based systems for controlled release.
  • Surface-functionalized with carboxyl, amine, or PEG handles.
  • Used for small-molecule, peptide, and nucleic-acid delivery applications.
Magnetic Nanoparticles (Fe3O4 / SPIONs)
  • Dextran, silica, or polymer coatings with carboxyl or amine groups.
  • Designed for magnetic separation, cell sorting, and target enrichment.
  • Options for MRI contrast and image-guided delivery studies.
Quantum Dots & Fluorescent Nanoprobes
  • Polymer-encapsulated quantum dots with carboxyl or amine surfaces.
  • High-brightness, photostable labels for imaging and multiplex assays.
  • Conjugation to antibodies, peptides, or nucleic acids.
Carbon Nanomaterials
  • Graphene oxide and functionalized carbon nanotubes with carboxyl and hydroxyl groups.
  • High surface area and conductivity for electrochemical and FET-based biosensors.
  • Can be combined with metal or polymer coatings for hybrid architectures.

Conjugation Chemistries

We use a toolbox of orthogonal chemistries to attach ligands and payloads while preserving nanoparticle stability and biological function. Buffer conditions, linker length, and reaction stoichiometry are optimized together for each nanomaterial and application.

Thiol–Gold & Au–Ligand Interfaces
  • Covalent attachment of thiolated DNA, peptides, and cysteine-containing proteins to AuNPs.
  • Backfilling with PEG or small-molecule thiols to control density and reduce nonspecific binding.
  • Critical for lateral-flow labels, plasmonic sensors, and CT/photoacoustic probes.
EDC/NHS Amide Coupling
  • Carboxyl-activated nanoparticles coupled to primary amines on proteins, peptides, or amine-oligos.
  • Used on silica, polymeric, and magnetic nanomaterials.
  • pH and buffer composition tuned to maximize activation while limiting hydrolysis and crosslinking.
Electrostatic & Adsorptive Strategies
  • Physical adsorption on silica or carbon surfaces when reversible or screening-friendly attachment is desired.
  • Useful for early feasibility work and rapid label generation.
  • Often combined with covalent routes in later-stage optimization.
Maleimide–Thiol & Site-Directed Installs
  • Maleimide-functional nanoparticles coupled to free thiols on cysteines or engineered tags.
  • Enables oriented antibody and Fab display to preserve binding domains.
  • Compatible with mixed surfaces that host both amine and thiol handles.
Click & Bioorthogonal Chemistry
  • Copper-free SPAAC and iEDDA reactions for sensitive proteins, cells, and in vivo work.
  • Azide–alkyne or tetrazine–TCO pairs used for modular, two-step assembly.
  • Supports multivalent architectures and sequential labeling strategies.
Biotin–Streptavidin Bridges
  • Streptavidin-coated nanoparticles combined with biotinylated ligands for plug-and-play loading.
  • Enables easy swapping of capture or detection reagents without rebuilding the nanoparticle core.
  • Useful in discovery, assay development, and modular kit designs.

For each project we typically propose a primary conjugation route plus at least one backup strategy, then confirm performance via small-scale scouting before committing to larger batches.

Applications

Drug Delivery & Theranostics
  • Loading of small molecules, peptides, or nucleic acids into polymeric or silica nanoparticles.
  • Surface decoration with targeting ligands (antibodies, peptides, sugars) for cell- or tissue-specific delivery.
  • Control of release profiles via polymer composition, crosslinking, and shell thickness.
Biosensing & Diagnostics
  • Gold nanoparticle conjugates for colorimetric and plasmonic readouts.
  • Magnetic nanoparticle conjugates for immunomagnetic separation and bead-based immunoassays.
  • Quantum dot and fluorescent nanoprobe conjugates for high-sensitivity multiplex detection.
Molecular Capture & Enrichment
  • Oligo-functionalized silica or magnetic nanoparticles for DNA/RNA capture and depletion.
  • Peptide- or protein-coated nanoparticles for phosphoprotein, glycoprotein, or interactome profiling.
  • Integration with NGS, MS, or imaging-based downstream analytics.
Imaging & Tracking
  • SPION-based conjugates for MRI contrast and cell tracking.
  • Gold and heavy-metal nanoparticles for CT and photoacoustic imaging.
  • Fluorescent nanoparticle labels for in vitro and in vivo imaging studies.
Cellular & Mechanistic Studies
  • Targeted nanoparticles for receptor trafficking and uptake studies.
  • Multivalent ligands for signaling pathway modulation and clustering experiments.
  • Nanoparticle-based pull-down and co-localization systems.

We align nanoparticle size, surface chemistry, and ligand density with your readout (optical, magnetic, electrochemical, imaging) and sample type (buffer, serum, plasma, cells, tissue models, etc.) to generate data that translates.

Technical Summary

Typical Workflow
  • Intake: nanoparticle platform selection, ligand review, and risk register.
  • Scout: buffers, chemistries, and PEG/linker architectures screened at small scale.
  • Build: scale-up using locked protocols with in-process DLS and activity checks.
  • QC: confirm particle size, charge, loading, activity, and stability.
  • Transfer: documentation and tech transfer for internal or partner manufacturing.
Controls & Comparators
  • Unconjugated nanoparticles to define baseline stability and background.
  • PEG-only and ligand-only controls to deconvolute nonspecific signals.
  • Alternative chemistries (e.g., thiol–gold vs. EDC/NHS) in early design-of-experiments.
  • Stress and freeze–thaw testing to emulate real storage and shipping conditions.
Analytics
  • DLS and PDI to monitor size distribution and aggregation.
  • Zeta potential to track surface charge and colloidal stability.
  • UV–Vis, fluorescence, or elemental analysis for loading and integrity.
  • Functional binding and activity assays in relevant buffers and matrices.

Nanoparticle Prep & Surface Design
  • Match size and coating to route of administration, assay type, and clearance expectations.
  • Use PEG length and density to balance stealth behavior and ligand accessibility.
  • Control ligand-to-particle ratio to avoid overloading and steric hindrance.
  • Choose buffers that maintain colloidal stability during conjugation and storage.
QC & Stability
  • Track changes in size, PDI, and zeta potential over time and after stress.
  • Monitor free vs. bound ligand to confirm loading and leaching behavior.
  • Test performance in final diluent or biological matrix, not just in ideal buffers.
  • Define acceptance criteria around functional activity, not only total protein coupled.

Nanoparticle
Type (gold, silica, SPION, polymeric, carbon), size range, coating, and intended use.
Ligand / Payload
Antibody, protein, peptide, oligo, drug; sequence/ID; preferred handle (amine, thiol, biotin, click tag).
Assay / Application
Readout (colorimetric, fluorescence, MRI, CT, electrochemical), in vitro vs. in vivo, and sample matrix.
Specs
Target particle size/PDI, required shelf-life, lot size, regulatory context, and performance criteria.

FAQ

How do I choose the right nanomaterial platform?

Choice depends on application and readout. Gold nanoparticles are preferred for optical assays, silica and polymer particles for drug loading, SPIONs for magnetic and MRI-based work, and carbon materials for electrochemical sensing. We can recommend a platform based on your use case and constraints.

Which conjugation chemistry should I use?

For gold, thiol–gold chemistries are usually first-line. For carboxylated nanoparticles we often use EDC/NHS; for controlled, site-directed installs we use maleimide–thiol or click chemistry. Biotin–streptavidin is ideal when you want modular, swap-able ligands. We review your ligand structure and application before recommending a route.

How do you control aggregation and stability?

Aggregation is managed through choice of surfactant/PEG, ionic strength, pH, and ligand loading. We monitor size and PDI during development and adjust buffers, linker density, and blocking strategies to maintain colloidal stability under storage and use conditions.

Can you perform site-specific or oriented conjugation?

Yes. We use cysteine engineering, maleimide–thiol chemistry, bioorthogonal handles, and tagged ligands (e.g., His, biotin) to increase orientation control and preserve activity of antibodies, enzymes, and other proteins.

What information do you need to start a project?

We typically ask for nanoparticle specs (type, size, coating), ligand identity and format, the intended application, sample matrix, and any performance targets (signal-to-background, release profile, stability, regulatory needs). Existing data or protocols are also very helpful.

Do you support OEM, kit, or translational development?

Yes. We support OEM and kit developers and can align batch sizes, documentation, and stability programs with your internal quality and regulatory requirements, including lot-release and comparability studies.

Contact

Talk to Our Nanomaterial Conjugation Team

Share your nanoparticle platform, ligand details, application, and performance goals. We’ll recommend a conjugation route, surface chemistry, and QC package, then return a project quote or feasibility assessment.

Request a Quote Feasibility Check OEM / Kit Partner Sample Submission
Email: sales@biosyn.com
Phone: +1-972-420-8505
Full Name *
Email *
Company / Institution *
Phone *
Nanomaterial platform *
Ligand / payload type
Project phase
Message
Attach file (optional)

By submitting, you agree to be contacted regarding your request.

Email Instead

Recommended Reading

  1. Hermanson, G. T. Bioconjugate Techniques. Academic Press — Comprehensive reference for conjugation chemistries and analytical methods.
  2. Peer, D. et al. Nanocarriers as an emerging platform for cancer therapy. — Overview of nanoparticle-based drug delivery strategies.
  3. Labhasetwar, V., & Leslie-Pelecky, D. L. Biomedical Applications of Nanotechnology. — Discussion of magnetic, polymeric, and metallic nanomaterials.
  4. Nel, A. E. et al. Understanding biophysicochemical interactions at the nano–bio interface. — Guidance for designing biologically compatible nanoconjugates.
  5. Rzigalinski, B. A. & Nelson, B. C. Nano-enabled drug delivery. — Practical considerations for formulation and stability.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

Quick Links