Surface & Solid-Support Conjugation Services — Beads, Plates, Chips & Resins

High-loading, low-background conjugation for beads, plates, chips, and resins — engineered with EDC/NHS,
maleimide, click, and biotin–streptavidin chemistries and supported by full QC.

ISO 9001:2015 / ISO 13485:2016
45+ Years
Assay & IVD Support
Texas, USA
Confidential & IP-Protected

Overview

Our surface & solid-support conjugation services immobilize antibodies, proteins, peptides, and oligonucleotides on magnetic beads, microplates, biosensor chips, and chromatography resins. We engineer high-loading, low-background surfaces using EDC/NHS, maleimide, click, and biotin–streptavidin chemistries with full QC support.

Our platform focuses on maximizing functional loading, minimizing non-specific binding, and ensuring robust lot-to-lot reproducibility. We match the right surface, linker length, and blocking strategy to your assay readout and sample matrix, then verify performance with targeted controls and QC analytics.

Surface / Solid Support
Magnetic Beads · Plates · Chips · Resins
Conjugation Chemistry
EDC/NHS · Maleimide · Click · Biotin
Immobilized Ligand
Antibodies · Proteins · Peptides · Oligos

We also offer complementary carrier delivery systems (ADC & LNP) and bioconjugation services that integrate with these surface & solid-support conjugation projects.

Assay-Ready Surfaces

Surfaces optimized for ELISA, bead-based immunoassays, NGS capture, and biosensors with defined coating density and blocking.

Tuned Binding & Background

Use of spacers, PEGs, and tailored blocking buffers to maximize signal-to-noise across real-world samples.

Documentation & Scale

Lot-controlled production with documentation suitable for kit developers, OEM partners, and regulated environments.

Surface & Solid-Support Platforms

Magnetic Beads & Microspheres
  • Carboxyl, amino, streptavidin, epoxy and specialty bead chemistries.
  • Sizes tuned for high-throughput automation and rapid wash steps.
  • Ideal for bead-based immunoassays, NGS and oligo capture, and enrichment workflows.
Planar Chips & Biosensor Surfaces
  • Glass and silicon slides for microarray and imaging-based readouts.
  • Gold or sensor-specific surfaces for SPR/BLI and electrochemical detection.
  • Controlled ligand orientation to preserve kinetics and binding profiles.
Microplates
  • High-binding and special-purpose plates for ELISA and multiplex assays.
  • Streptavidin-coated formats for modular biotin-based coating strategies.
  • Available with custom coating density, blocking, and packaging.
Chromatography Resins
  • Agarose/acrylamide matrices for affinity, depletion, and pull-down workflows.
  • Custom ligand coupling, capacity tuning, and regeneration testing.
  • Suitable for proteomics, interactomics, and preparative purification.

Conjugation Chemistries

We apply a toolbox of well-characterized, assay-compatible chemistries to achieve robust attachment while preserving ligand function. Surface activation, ligand formulation, and reaction conditions are optimized together to minimize hydrolysis and side reactions.

EDC/NHS & Amide Coupling
  • Carboxyl surfaces activated with EDC/NHS in controlled buffers.
  • Coupling to primary amines on antibodies, proteins, peptides, or amine-modified oligos.
  • Buffer and pH selection to balance activation efficiency vs. hydrolysis.
Maleimide–Thiol & Cysteine Targeting
  • Oriented attachment via free cysteines or engineered thiol handles.
  • Enables controlled display of antibody Fabs, peptides, and proteins.
  • Compatible with mixed surface strategies (amine + thiol installs).
Epoxy & Aldehyde Surfaces
  • Direct nucleophilic addition from amine-containing ligands.
  • Robust, hydrolytically stable covalent attachment for long shelf-life formats.
  • Useful for both protein-based ligands and small-molecule capture chemistries.
Biotin–Streptavidin & Click
  • Biotin–streptavidin for high-affinity, modular, and reversible loading.
  • Copper-free click chemistries (SPAAC, iEDDA) for sensitive or live systems.
  • Supports multi-step and dual-ligand strategies with orthogonal handles.

For each project we recommend a primary chemistry plus alternatives, with small-scale scouting to confirm coating density, activity, and background under your exact assay conditions.

Applications

Immunoassays & Diagnostics
  • ELISA plates and magnetic beads for single-analyte or multiplex biomarker panels.
  • Capture and detection antibody coupling with tuned orientation and density.
  • Blocking and wash optimization to minimize matrix effects and background noise.
NGS & Nucleic Acid Capture
  • Oligo-functionalized beads and chips for target enrichment and depletion.
  • Biotin–streptavidin and click strategies for modular library prep workflows.
  • Performance tested with representative libraries, buffers, and sample types.
Biosensors & Microarrays
  • SPR/BLI sensor chips with immobilized antibodies, proteins, or oligos.
  • Protein and peptide microarrays for profiling, epitope mapping, and screening.
  • Regeneration and reuse strategies validated where the platform allows.
Affinity Purification & Pull-Down
  • Custom resins for depletion, enrichment, and interactome mapping.
  • Peptide- or protein-based ligands coupled at controlled capacities.
  • Stability and cleaning conditions mapped to your downstream analytics.

We design surfaces that align with your downstream readouts — fluorescence, chemiluminescence, colorimetric, MS, SPR/BLI, or imaging — and your real matrices (serum, plasma, lysate, etc.).

Technical Summary

Typical Workflow
  • Intake: surface selection, ligand review, and risk register.
  • Scout: small-format screening of chemistries, linker lengths, and blocking options.
  • Build: scale-up using locked protocols with in-process QC checkpoints.
  • QC: verify coating density, activity, background, and stability.
  • Transfer: documentation for tech transfer or OEM/kit integration.
Controls & Comparators
  • Negative and isotype controls for immunoassays and biosensors.
  • Uncoated or minimally coated surfaces to baseline background.
  • Alternative chemistries (e.g., EDC/NHS vs. maleimide) in early scouting.
  • Stress and shipping simulations for long-distance distribution.
Analytics
  • Functional binding tests (e.g., antigen/ligand titrations).
  • Signal vs. background profiling in relevant matrices.
  • Lot-to-lot comparisons over defined acceptance criteria.
  • Stability and shelf-life monitoring under recommended storage conditions.

Surface Prep & Coating
  • Match surface chemistry (carboxyl, amino, epoxy, streptavidin) to ligand and assay readout.
  • Choose buffers free of competing amines or high salt during activation steps.
  • Control ligand concentration and reaction time to avoid overcrowding and steric hindrance.
  • Incorporate PEG and spacer arms where additional reach or reduced non-specific binding is needed.
QC & Stability
  • Functional tests with positive/negative controls across multiple runs.
  • Background and non-specific binding assessment with representative matrices.
  • Real-time and accelerated stability under shipping and storage conditions.
  • Lot release criteria defined around performance, not just total protein coupled.

Ligand
Type (antibody, protein, peptide, oligo), sequence/ID, desired immobilization handle (amine, thiol, biotin, tag).
Surface & Format
Preferred surface (beads, plate, chip, resin), required volume or number of wells/particles, and any vendor constraints.
Assay & Matrix
Readout (ELISA, fluorescence, chemiluminescence, SPR/BLI, MS), sample type (serum, plasma, lysate, etc.), and any regeneration requirements.
Specs
Desired shelf-life, lot size, and performance criteria (signal-to-background, CVs, etc.).

FAQ

How do I choose the right surface — beads, plates, chips, or resins?

It depends on the assay format, throughput, and readout. Beads excel in automation and wash steps, plates are standard for ELISA, chips support microarrays and imaging, and resins are best for depletion/enrichment. We can recommend a platform based on your workflow and sample matrix.

Which chemistry is best for my ligand?

For amine-rich proteins, EDC/NHS or epoxy/aldehyde works well; for cysteine-targeted or oriented installs we use maleimide–thiol; for modular systems we often use biotin–streptavidin or copper-free click. We’ll review your ligand sequence and format before proposing a route.

Can you help reduce background in my current assay?

Yes. Background is often a combination of surface chemistry, coating density, and blocking/wash conditions. We can adjust ligand loading, introduce PEG spacers, and screen blocking buffers tailored to your sample matrix.

Do you support OEM or diagnostic kit development?

We routinely support kit and OEM partners with defined lot sizes, stability programs, and documentation suitable for regulated environments. We can align lot release criteria with your internal specifications.

What information do you need to start a project?

Provide ligand details, preferred surface and format, assay readout, sample matrix, and any performance targets (e.g., dynamic range, CVs, shelf-life). If you have an existing assay, sharing your current protocol and pain points is very helpful.

Can you work with my proprietary ligands and under NDA?

Yes. We routinely work under NDA/MSA and treat all project materials and results as confidential. IP and ownership terms are defined up front as part of project onboarding.

Contact

Talk to Our Bioconjugation Team

Share your surface type, ligand details, assay format, and performance goals. We’ll recommend a conjugation route, surface chemistry, and QC package, then return a project quote.

Request a Quote Feasibility Check OEM / Kit Partner Sample Submission
Phone: +1-972-420-8505
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Recommended Reading

  • Hermanson, G. T. (2013). Bioconjugate Techniques, 3rd Ed. Academic Press — Comprehensive coverage of surface activation chemistries and analytical methods.
  • Nguyen, U. T. T. et al. (2009). Protein and peptide immobilization on solid supports. — Overview of amine, thiol, and affinity-based immobilization strategies.
  • Rodriguez, E. et al. (2017). Magnetic beads for immunoassay development. — Practical insights into bead size, surface chemistry, and assay performance.
  • Cooper, M. A. (2002). Label-free biosensors: Techniques and applications. — SPR/BLI surface considerations and regeneration strategies.
  • Mosbach, K. & Ramström, O. (1996). The emerging technique of molecular imprinting. — Concepts for highly selective solid-phase recognition.

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