Custom Aptamers — Precision Binding, Unlimited Potential

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Custom Aptamer Synthesis

Design, SELEX selection, chemical modification, and conjugation — end-to-end support from research to GMP.

Overview

Aptamers are short, single-stranded DNA or RNA molecules that fold into defined 3D structures to bind targets with high affinity and specificity — including proteins, small molecules, ions, and whole cells.

Bio-Synthesis provides custom aptamer solutions: from design and SELEX selection through chemical modification, labeling, conjugation, purification, and comprehensive QC.

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At-a-Glance

  • Formats: DNA or RNA aptamers; LNA/BNA mixmers on request
  • Selection: Protein-, Cell-, or Small-Molecule SELEX
  • Labels: dyes, biotin, haptens; click-ready handles
  • Conjugations: PEG, lipids, peptides, nanoparticles
  • Purification: HPLC standard; IE-HPLC/PAGE optional
  • QC: MS + analytical HPLC; optional binding/affinity assays

Applications

  • Biosensors & ELONA
  • Lateral flow assays
  • Imaging probes
  • Targeted delivery & receptor antagonism
  • Molecular decoys
  • Affinity capture/purification
  • Environmental & toxin detection
  • Cell sorting & phenotyping
  • Microarray & multiplex diagnostics

Custom Synthesis Options

Parameter Options
Backbone DNA or RNA; optional LNA/BNA mixmers
Length 25–100 nt typical (custom outside range)
Selection (SELEX) Protein-SELEX, Cell-SELEX, Small-Molecule SELEX; counter-selection to reduce off-targets
Chem Modifications 2′-F, 2′-OMe, PS, capping; PEGylation; cholesterol/lipid
Labels/Tags Fluorophores, biotin/digoxigenin, quenchers, affinity tags
Conjugations Peptides, antibodies, nanoparticles; click-chemistry handles
Purification RP-HPLC (standard), IE-HPLC, PAGE
Scale 1 mg to grams; tubes or 96-well plates
Deliverables Lyophilized or in buffer; full documentation

Popular Modifications

Category Examples Typical Uses
Stability Enhancers 2′-F pyrimidines, 2′-OMe, LNA/BNA mix, phosphorothioate ends Nuclease resistance, improved in vivo durability
Pharmacokinetics PEG(5–40k), cholesterol, lipids Half-life extension, formulation tuning
Detection FAM, HEX, Cy3/Cy5, ATTO, Alexa Fluor® Fluorescent imaging, FRET, real-time assays
Affinity/Handles Biotin (C6/TEG), DIG, DNP; amine/thiol/azide/alkyne Capture, conjugation, surface coupling
Quenchers BHQ-1/2/3, Iowa Black, Eclipse Molecular beacons, signal-on/off probes

Need unusual chemistries or dual-function constructs? Share your schematic—we’ll review feasibility and route.

Quality Assurance

  • Mass spectrometry (MALDI/ESI) for MW confirmation
  • Analytical HPLC profile and purity
  • Optional binding tests (Kd estimation, competition assays)
  • RUO documentation; GLP/cGMP support on request

Typical Turnaround

Custom synthesis of known aptamer sequences: 1–2 weeks. Full SELEX campaigns vary (4–10+ weeks) based on target complexity and screening depth.

Timelines depend on target type, modifications, and verification assays. Rush options may be available.

How to Order

  1. Share your target, matrix, and assay format.
  2. Choose backbone (DNA/RNA) and desired modifications/conjugations.
  3. Select SELEX strategy (if required) and QC readouts.
  4. Receive a same-day quote and projected timeline.

NDA-friendly consultations available. Include any IP constraints or literature references.

Specs Checklist

  • Target identity and purity (protein/cell/small molecule)
  • Assay conditions (buffer, pH, temperature, matrix)
  • Performance goals (Kd, specificity, on/off-rate)
  • Backbone and chemical mods; labeling/conjugations
  • Scale, purification, and required documentation
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Aptamer Technology & Benefits

SELEX Overview: Aptamers are discovered via SELEX (Systematic Evolution of Ligands by EXponential enrichment), which iteratively enriches high-affinity binders from libraries (1013–1015 variants). Counter-selection removes off-target binders; winners are cloned and sequenced to identify lead candidates.

Why Aptamers Work
  • Folded 3D structures: Loops, stems, bulges, and G-quadruplexes create precise binding pockets.
  • Chemical tunability: 2′-F/2′-OMe/LNA/BNA and terminal capping improve stability and affinity.
  • Conjugation-ready: Dyes, PEG, lipids, peptides, and nanoparticles enable multi-modal functions.
  • Batch consistency: Fully synthetic production ensures reproducibility across lots.
SELEX Workflow (Typical)
  • Library design → target incubation → partition → amplification
  • Counter-selection to remove matrix binders
  • Next-generation sequencing & motif analysis
  • Lead synthesis, truncation/optimization, and validation
Benefits at a Glance
  • High affinity & specificity: pM–nM binding, antibody-like recognition
  • Chemical stability: Works across broad pH/temperature; freeze–thaw tolerant
  • Fast development: Rapid iteration once a lead is identified
  • Scalable manufacturing: From μg to g; RUO to cGMP
  • Versatility: Binds proteins, small molecules, cells, and surfaces
  • Low immunogenicity potential: Non-proteinaceous, fully synthetic

Need therapeutic profiling (PK/PD, serum stability, toxicity)? We can integrate study-ready chemistries and documentation.

Use Case Why Aptamers Help Typical Setup
Point-of-care diagnostics Rapid, specific binding with stable storage Biotinylated aptamer + dye label; lateral flow or biosensor format
Targeted delivery Selective cell-surface binding and internalization Aptamer–drug or aptamer–nanoparticle conjugate; PEG for PK
Affinity capture & enrichment High specificity for purification/cleanup Biotin-TEG tag + streptavidin capture; orthogonal elution
Live-cell imaging Fluorescent, non-immunogenic recognition Cy5/Alexa-labeled aptamer; optional quencher for background control
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FAQ

Do you support GMP production?

Yes. We support RUO through cGMP with appropriate QA oversight and documentation.

Do I need SELEX, or can you synthesize a known aptamer?

We do both. Provide a literature sequence for direct synthesis, or engage our SELEX service to discover new binders to your target.

How do you improve serum stability?

We combine 2′-F/2′-OMe/LNA/BNA, terminal capping, and PEGylation or lipids to enhance nuclease resistance and pharmacokinetics.

hat affinity can I expect?

Affinity depends on target complexity. Many projects reach low-nM to sub-nM Kd after optimization.

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Complete the form to receive a tailored quote. Your request will be emailed to info@biosyn.com and logged to your CRM endpoint (configure below).

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PNA Sequence(s)
Labels / Linkers / Conjugations
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Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.