Header

Header

Header

Oligonucleotide Modification by Application

Explore how chemical modifications enable real-world use cases—from gene silencing and targeted delivery to DNA repair, crosslinking, controlled release, and assay detection.

ISO 9001:2015 / ISO13485:2016 45+ Years of Expertise U.S.A. Facilities-Texas GLP/GMP-Aligned

A Comprehensive Approach to Application-Driven Oligo Modifications

Unlock the full potential of your oligonucleotides with application-specific modifications. Whether you're designing probes for diagnostics, developing RNA therapeutics, or engineering gene regulators, selecting the right modification is key to maximizing efficiency, stability, and impact.

BSI remains committed to delivering high-quality, custom-modified oligonucleotides backed by expert technical support. Explore our diverse and continuously growing portfolio of over 3000 oligo modifications, designed to meet your application’s exact demands.

If the modification you need is not listed, please don’t hesitate to contact us—our team is ready to assist with custom solutions tailored to your specific requirements.

Application Areas

Antisense oligos / Gapmers

Custom antisense gapmers designed to silence target RNA via RNase H, combining nuclease resistance with potent gene knockdown.

RNAi – siRNA

Chemically modified siRNA duplexes optimized for stability, delivery, and efficient RNA interference.

Delivering and Targeting

Conjugation strategies (GalNAc, cholesterol, fatty acids, peptides, PEG) that guide oligonucleotides to specific cells and tissues.

Conjugation to Antibody (ADC/AOC)

Antibody–oligonucleotide conjugates for targeted delivery and combined therapeutic or diagnostic applications.

Epigenetics

Modified oligos to study or modulate DNA methylation, histone interactions, and chromatin remodeling.

Cell Delivery and Uptake

Use of CPPs, lipids, vitamins, and hydrophobic moieties to enhance cellular uptake and endosomal escape.

DNA Damage Repair

Oligos that modulate repair pathways, correct mutations, or act as templates for homology-directed repair.

DNA Cross Linking and Ligation

Crosslinking bases and reactive handles to create covalent DNA–DNA or DNA–protein linkages for structural biology studies.

Mutagenesis and Methylation

Modified bases that probe mutagenesis, introduce site-specific changes, or analyze methylation-dependent processes.

Chemical Ligation

Reactive modifications enabling enzyme-free ligation and assembly using click chemistry or other orthogonal chemistries.

Colorimetric Detection

Labels and chemistries that produce visible signals for rapid, low-cost detection in assays and diagnostics.

Ligation Blocking

Termini blockers and spacers that prevent unwanted extension or ligation in molecular biology workflows.

Controlled Release / Cleavable Linkers

Redox-, enzyme-, pH-, or photo-cleavable linkers for controlled oligo release in cells or therapeutic settings.

Structure–Activity Study

Modified oligos used to explore sequence–function relationships, structural dynamics, and activity profiles.

Bioorthogonal Probes

Click-compatible handles for labeling, imaging, and capture without perturbing native biology.

Chain Terminators

Modified nucleotides that terminate polymerase extension—used in sequencing, mutagenesis, and drug studies.

Caged Oligonucleotide

Photocaged oligos carrying light-sensitive groups for spatiotemporal control of gene expression or probe activation.

Electrochemical Sensing Readout

Redox oligos (MB, Fc) for electrochemical sensing—high S/N DPV/SWV, stable linkers, gold/SP electrodes.

Need help mapping an application to the right chemistry?

Our scientists can recommend modification patterns and conjugates aligned to your target, route, and assay.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.