Build diversity with IUPAC degenerate bases and per‑cycle mixture control. From randomers/UMIs and barcodes to NNK/NNS codon mutagenesis and targeted biased mixes, we manufacture high‑complexity pools with validated composition and full QC.
Bio‑Synthesis provides end‑to‑end degenerate base (IUPAC) & mixed‑nucleotide services for UMIs/barcodes, randomers, and NNK/NNS codon mutagenesis. We handle design consultation (IUPAC schemes, biased ratios, NNK/NNS vs. trimer strategies), per‑site dosing with compensation for chemistry bias, custom synthesis from µmol → multi‑gram, and a full QC package.
Deliverables can include composition reports (trityl monitoring), optional NGS validation (sentinel or full‑length), CoA & method summaries, and RUO → GMP‑like documentation. We also offer kitting/aliquoting, barcoding, and logistics support. Inosine and universal base options are available where pairing tolerance is required.
During each coupling, we meter A/C/G/T according to your ratio. Ratios can vary by position in one sequence.
Compensate for reactivity differences; trityl monitoring keeps composition near spec. Optional NGS confirms base distributions.
For maximum diversity, desalt is typical. If HPLC is required, expect some diversity loss; we can tune gradients to mitigate.
Error‑correction, deduplication, and molecule tracking with NL randomers or structured barcodes.
NNK/NNS codon randomization for libraries; trimer codon mixes reduce bias and stop codons.
Degenerate positions improve inclusivity; inosine/universal bases can be used where tolerance is needed.
IUPAC codes define base mixtures (e.g., R=A/G, Y=C/T). We meter A/C/G/T per cycle to create controlled variability.
NNK (K=G/T) and NNS (S=G/C) both reduce stop codons vs NNN; we can also supply trimer codon mixes for uniform AA coverage.
Yes. We dose custom percentages per position (e.g., A:10/C:40/G:40/T:10) and report composition on request.
Somewhat. Desalt maximizes representation; tuned gradients can mitigate loss if HPLC/UPLC is required.
Yes. Sentinel‑site or full‑length NGS can quantify base distributions and confirm dosing accuracy.
Inosine provides wobble pairing tolerance; universal bases (e.g., 5‑nitroindole) help at uncertain positions but may lower Tm.
Send positions, IUPAC codes or % ratios, desired complexity, purification, and any NNK/NNS/trimer scheme.
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On request, we’ll align mixture specs and validation plans to your assay and instrument.
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