Reversible, light‑controlled moieties—azobenzene (incl. visible‑light variants), arylazopyrazole, diazocine, diarylethene, and spiropyran—to modulate hybridization, structure, and capture/release on demand.
Photoswitchable markers reversibly change conformation/absorption when illuminated, enabling optical control of hybridization, conformation, quenching, or capture. In oligonucleotides, azobenzene and related families can stabilize/destabilize duplexes in one state and reverse under another wavelength or via thermal relaxation—ideal for photo‑gated hybridization, switchable toeholds, aptamer capture/release, and molecular logic.
Trans often stabilizes; cis bends/destabilizes. 1–3 units tune Tm shift.
Red‑shift into visible; improves biocompatibility and penetration.
High‑contrast (AZP) or inverted stability (diazocine) for extended states or dark‑favored designs.
Bistable open/closed; excellent fatigue—great for many cycles.
SP↔MC switching couples optical contrast with environment sensitivity.
Internal base replacements or pendant/backbone linkers; multiple units amplify effects.
Toggle duplex formation or strand displacement with light.
Control binding and elution via light—no chaotropes or heat.
DTE/AZP support bistability and high cycle counts for logic/memory.
Choose photoswitch family (Azo/AZP/DTE/SP/Diazocine/HTI), placement (internal/base vs linker), and wavelengths/filters.
Synthesis with selected unit(s) → Purification (Desalt→HPLC/UPLC) → LC‑MS identity → Optional spectra & switching/fatigue tests.
Confirm λ/filters and cycle count; set visible/UV exposure as needed; finalize buffer and crosstalk/FRET checks.
Share sequence, wavelengths, placement, and cycle count—We’ll return a tuned design and QC plan.
Photoswitchables are reversible (toggle between states). Photocleavables are irreversible (bond is broken).
Yes—use visible‑light azobenzenes (e.g., o‑fluoro), arylazopyrazoles, diazocines, or hemithioindigo matched to your LEDs/filters.
One internal Azo can shift Tm; 2–3 spaced units amplify the effect. Exact shift depends on placement and sequence context.
Switching changes absorption/stacking and can alter FRET/quencher behavior. We can co‑design dye/quencher placement for robust signals.
On request, we’ll align literature‑style switching conditions and placements to your instrument.
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