Peptide-Protien Conjugation Services

Comprehensive peptide–protein conjugation with practical chemistry and fit-for-purpose QC

Chemically defined peptide–protein conjugates for research, diagnostics, and development-stage programs.

Overview

Peptide–protein conjugation (protein–peptide conjugation) is the covalent attachment of a synthetic peptide to a protein scaffold to create a single, chemically defined construct. Compared with non-covalent complexation, covalent conjugation supports reproducible characterization, controlled stoichiometry, and consistent performance across batches.

Bio-Synthesis provides end-to-end peptide–protein conjugation across carrier proteins, enzymes, antibodies (IgG, monoclonal antibodies, and fragments), and recombinant proteins. We also support protein labeling/tagging, crosslinking and interaction mapping, and development-stage therapeutic constructs.

Peptide–protein conjugation overview schematic
What we can control
  • Attachment site: Lys/amine, native or engineered Cys/thiol, click handles
  • Linker type: stable vs cleavable (where release concepts are required)
  • Process path: solution-phase coupling; peptide can be pre-functionalized during solid-phase synthesis
  • QC level: program-aligned identity/profile checks and documentation
Typical deliverables
  • Purified conjugate (as requested) and COA / data package
  • Conjugate profiling (e.g., SEC/HPLC) and integrity checks
  • Traceable production records (as requested)

Final acceptance criteria should be defined by intended use (research, diagnostic, development-stage).

Why Bio-Synthesis for peptide–protein conjugation?

Fit-for-purpose chemistry (not a kit)
  • Route selection: amine, thiol, and copper-free click based on your protein format and desired site control
  • Handle planning: peptide can be pre-functionalized during synthesis for cleaner conjugation
  • Linker options: stable spacers and cleavable designs when a release concept is required
Program-aligned QC and documentation
  • Characterization: conjugate profiling (SEC/HPLC) and integrity checks; LC–MS where applicable
  • Reproducibility: defined workflows to support consistent performance across batches
  • Communication: clear recommendations on input material (buffer, concentration) and success criteria

Share intended use and acceptance criteria (research, diagnostic, development-stage) so we can match the route and data package.

What we typically need to quote accurately
Protein type & format Buffer / stabilizers Concentration & volume Peptide sequence & handle Scale QC level

Peptide–Protein Conjugate Categories

A structured taxonomy helps search engines (and users) understand coverage across protein classes while keeping each topic distinct. Expand each category for typical formats, conjugation approaches, and applications.

Immunogenic peptide conjugation to carrier proteins for antibody generation and immunoassay development.

KLHBSAOVA CRM197Blue CarrierMAP
  • Use cases: antibody production, ELISA standards, immunoassays, epitope presentation
  • Common routes: amine coupling; thiol coupling for site-aware designs
  • QC: peptide loading/ratio guidance, identity checks, profile confirmation
View details

Peptide conjugation to enzymes for detection, signal amplification, affinity capture, or mechanistic studies.

HRP ALP β-Gal Dehydrogenases
  • Key constraint: preserve catalytic activity and avoid active-site obstruction
  • Common routes: amine coupling; selective thiol routes when available
  • QC: integrity profiling + optional activity retention check (if assay provided)
View details

Peptide conjugation to whole antibodies and antibody-derived formats, with optional fragmentation workflows.

IgG Monoclonal mAb Fab F(ab’)₂ scFv
  • Includes: peptide–IgG conjugation, peptide–mAb conjugation
  • Optional: antibody fragmentation → purification/conditioning → conjugation
  • Common routes: Cys/thiol and Cu-free click for improved site control; Lys for prototypes
  • QC: SEC/HPLC profiling; LC–MS where applicable; integrity checks
View details

Peptide conjugation to expressed proteins and domains from bacterial or mammalian expression systems.

Protein domains Receptors Ligands Growth factors
  • Good fit for: engineered handles (Cys/click-ready residues), site-defined attachment strategies
  • Common routes: thiol and click; amine coupling for broad compatibility
  • QC: integrity profiling; confirmation of conjugation and aggregation state
View details

Use peptide motifs as functional tags or handles on proteins for capture, detection, or modular assembly.

Affinity tags Reporter peptides Handle peptides
  • Examples: affinity/capture tags, detection peptides, click-ready peptide handles
  • Common routes: click-ready attachment; selective thiol coupling; amine for rapid labeling
  • QC: profile confirmation + identity checks aligned to construct type
View details

Peptide-based crosslinking for interaction capture and structural mapping workflows.

Photo-crosslinking Reactive motifs Defined spacers
  • Use cases: protein–protein interaction mapping, epitope mapping, MS-based analysis support
  • Common routes: incorporate crosslinker motifs into peptide; then conjugate to protein scaffold
  • QC: confirm incorporation/conjugation; provide guidance for downstream capture/analysis
View details

Development-stage peptide–protein conjugates intended for research and preclinical evaluation.

Targeting conceptsStable/cleavable linkersProgram-aligned QC
  • Scope: targeting peptides linked to protein scaffolds, modular constructs, evaluation batches
  • Common routes: site-defined thiol/click when possible; linker selection aligned to concept
  • QC: identity/profile, aggregation assessment, documentation per requested stage needs

Note: biological performance and safety claims are evaluated in your study system; we provide chemistry and analytics support.

View details

Common peptide–protein conjugation chemistries

Route selection depends on the protein scaffold, peptide handle position, desired site control, and downstream constraints. Below are commonly used, broadly compatible strategies.

Peptide–protein conjugation workflow showing peptide handle selection, conjugation chemistry, purification, and QC
Typical workflow: define peptide handle and protein format → select chemistry → purify → verify with fit-for-purpose QC.
Amine (Lys) coupling

Fast, robust option when exact site control is not required.

  • Targets accessible protein amines
  • Practical for immunogen and assay reagents
  • Conjugate distribution confirmed by profiling
NHS ester EDC coupling
Thiol (Cys) coupling

Improved site control using native or engineered cysteines.

  • Maleimide and thiol-reactive handles
  • Compatible with engineered site-specific strategies
  • Redox conditions selected to preserve integrity
Maleimide Thiol-alkylation
Click chemistry (Cu-free preferred)

High selectivity when azide/alkyne handles are used.

  • Copper-free strain-promoted workflows for proteins
  • Excellent for site-defined peptide handles
  • Useful for modular assembly and screening
DBCO BCN Azide
Linkers and spacers

Linker choice is application-dependent. We support stable spacers for durable constructs and cleavable designs when release is required. Spacer length can be tuned to reduce steric interference (e.g., near active sites or antibody paratopes).

Stable spacers Cleavable linkers (as needed) PEG spacers Self-immolative concepts (where applicable)
Discuss your peptide–protein conjugation project

Send your protein type and format, buffer/concentration, peptide sequence and desired handle, target scale, and QC/data package expectations. We’ll propose a practical conjugation route and verification plan.

FAQ

What proteins can you conjugate peptides to?
Do you provide antibody fragmentation and peptide conjugation?

Yes. We support antibody fragmentation (e.g., Fab or F(ab’)₂) followed by peptide conjugation using site-aware chemistries.

How do you choose the conjugation chemistry?

Chemistry is selected based on protein format, attachment site availability, peptide handle position, and downstream constraints (see conjugation chemistries).

What analytical characterization is provided?

Fit-for-purpose QC may include conjugate profiling (SEC/HPLC), identity confirmation, and integrity assessment (see workflow & QC).

How long does peptide–protein conjugation take?

Timeline depends on peptide length/handles, protein format and stability, conjugation chemistry, and the requested QC/data package. For planning, send the inputs in Request a quote and we’ll propose a practical route with an estimated schedule.

Can you work with customer-supplied proteins or antibodies?

Yes. We can work with customer-supplied antibodies and other proteins, provided they are supplied in labeling-compatible buffers. If needed, we can advise on purification or buffer exchange before conjugation.

High-quality starting material is critical for antibody–oligo conjugates. Buffers and stabilizers can interfere with labeling and should be removed prior to conjugation. Bio-Synthesis can review formulations and recommend purification or buffer exchange.

Please submit your antibody in one of the following formats:

  • 2 mg/mL in 0.05 M PBS, pH 7.2–7.4, containing 0.15 M NaClno additivessterile filtered; ship overnight on ice packs (no dry ice).
  • 2 mg/mL in 0.05 M PBS, pH 7.2–7.4, containing 0.15 M NaCl and 50% glycerolno other additives; ship overnight on ice packs or dry iceNote: The antibody will require purification prior to labeling.

The antibody must be in an amine-free buffer (e.g., no Tris) and free of additives that may interfere with labeling (e.g., azidegelatin). If you’re unsure, our technical team can advise on individual formulations.

Request a quote

What to send
  • Protein type/format (carrier, enzyme, IgG/mAb/fragment, recombinant)
  • Buffer composition, stabilizers, concentration, and volume
  • Peptide sequence and desired handle/attachment site
  • Target scale and required deliverables (purification/QC/documentation)
Next steps

Share your design inputs and timeline. We’ll recommend a practical conjugation route and a fit-for-purpose verification plan.

Recommended reading

Background references for planning conjugation strategies, protein modification, and characterization.

  • Hermanson, G. T. Bioconjugate Techniques (3rd ed.). Book listing
  • Site-specific antibody conjugation strategies overview (mAbs, 2014). PDF
  • General protein labeling and modification concepts (technical overview). Read
  • Crosslinking/MS concept overview (primer). Handbook

References are provided for scientific context. Acceptance criteria and release tests should be defined per intended use.

Why Choose Bio-Synthesis

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