Purpose-built base lesions and methylated nucleobases for site-specific mutagenesis, DNA damage & repair studies, and epigenetic mechanism mapping. End-to-end design, synthesis, purification, and QC—from RUO to GMP-like supply.
Bio-Synthesis provides a curated portfolio of methylated oligonucleotides and oligonucleotide methylation chemistries—spanning mutagenesis and methylation base modifications—spanning methylated purines/pyrimidines, oxidative lesions, and deamination/depurination models—to enable precise placement of biologically relevant adducts within DNA. These site-specific lesions power studies in polymerase fidelity, BER/NER/MMR pathway mapping, epigenetic readouts, and structure–function analysis.
Lesion choice, strand/position & sequence context; duplex/plate layout aligned to your readout.
Custom amidites/CPGs, specialty coupling; HPLC/UPLC or PAGE, desalting & lyophilization.
LC-MS confirmation, duplex assembly & Tm; optional endotoxin and nuclease challenge.
µmol → multi-gram with RUO → GMP-like documentation aligned to your QMS.
Designing oligonucleotides with mutagenesis and methylation lesions requires balancing **biological relevance**, **stability**, and **assay compatibility**. Bio-Synthesis integrates advanced solid-phase synthesis, specialty amidites, and duplex assembly workflows to ensure your constructs model damage and epigenetic marks with precision.
Tell us your readout (fidelity, repair, blocking, reader binding) and we’ll recommend placement, purification, and QC—then scale from mg to multi-gram.
Alkylation: O6-Me-dG, O4-Me-dT, O2-Me-dT, 1-Me-dA, 1-Me-dG, N3-Me-dC. Oxidation: 8-oxo-dG/dA, thymidine glycol, 5-OH-dC/dU, 5-hm-dC/dU.
AAAAYes—annealed duplexes, pooled libraries, or 96/384-well plates with barcodes. We can mirror your LIMS IDs and provide sequence maps
HPLC/UPLC and LC-MS are standard. Optional PAGE, endotoxin testing, and custom LC-MS methods are available for labile lesions.
Yes—ISO 9001/13485 operations with RUO→GMP-like documentation, batch records, and traceability to support regulated workflows.
Methylated oligonucleotides enable controlled base modifications (e.g., O6‑Me‑dG, O4‑Me‑dT, N3‑Me‑dC) to probe polymerase fidelity, mismatch repair, and lesion bypass.
Yes. We provide custom oligonucleotide methylation and synthesis with HPLC/UPLC and LC‑MS QC, RUO→GMP‑like documentation, and annealed duplex delivery on request.
Use methylated oligonucleotides when specifying a finished reagent; use oligonucleotide methylation to discuss the process. We can help translate your assay needs into the right construct.
Please avoid confidential details; we can arrange an NDA if needed.
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