Enzyme-safe chemistries for controlled peptide attachment and reproducible characterization
Peptide–enzyme conjugates are widely used in analytical and diagnostic formats because they combine catalytic signal generation with targeting or affinity functions. Established enzyme conjugation strategies and reporting formats are documented in the bioconjugation literature (e.g., Hermanson, Bioconjugate Techniques).
Bio-Synthesis provides custom peptide–enzyme conjugation services using enzyme-compatible chemistries and practical attachment strategies (site-defined or controlled labeling), followed by purification and analytical verification aligned to your application and program stage. These chemically defined peptide–enzyme conjugates often used for diagnostics, assays, and biosensor workflows—with controlled attachment, purification, and documentation aligned to your program stage.
Typical workflow for peptide–enzyme conjugate synthesis, purification, and analytical control.
Scope note: This page focuses on chemistry, workflow, purification, and analytical qualification. Enzyme activity retention is application-dependent and is discussed as a design consideration rather than a guaranteed outcome.
Related services: Peptide Modifications, Click Chemistry Peptides, Oligonucleotide Conjugation.
Design choices emphasize attachment site, linker/spacer architecture, and reaction conditions to reduce steric interference and preserve enzyme function.
Enzyme reporters (e.g., HRP/ALP) coupled to peptides for capture, spacing, or amplification concepts.
Peptide handles support oriented tethering, spacing, or surface attachment for reproducible sensor builds.
Peptide–enzyme conjugates for method development, binding studies, and controlled labeling workflows.
We support peptide conjugation to common enzyme reporters and enzyme-based systems used in analytical and diagnostic workflows. Additional targets can be evaluated based on structure, reactive residue availability, and intended readout.
If you have a specific enzyme/protein not listed, share the target and constraints—we’ll recommend a practical attachment plan.
Used when a defined cysteine handle is available or engineered to support site-defined attachment.
A common route for clean, chemoselective coupling with minimal side reactions.
Used for enzyme labeling workflows when site-defined handles are constrained.
We can align conjugation design to your assay format (solution-phase vs immobilized) and reporting needs.
Activity assays can be discussed as a program-specific requirement; analytical verification focuses on identity and conjugation profile.
Bio-Synthesis is committed to Total Quality Management (TQM) to assure customer satisfaction. Analytical checks are performed following peptide synthesis and peptide–enzyme conjugation, and purification/QA procedures are applied to support consistent quality.
Our quality system follows ISO 9001–aligned practices, with release criteria and documentation scaled to the intended use and program stage.
Last updated: 2026-01-31
For the fastest quote, send the enzyme/protein target, peptide sequence and attachment preference (or constraints), preferred chemistry (or “recommend”), and quantity/purity targets.
Not sure which route fits your constraints? Send target + peptide + constraints—we’ll propose an enzyme-compatible plan.
Conjugation design emphasizes attachment site planning, spacer selection, and enzyme-compatible reaction conditions to reduce steric interference. Retained activity is enzyme- and design-dependent.
Yes. When a defined handle is available (e.g., cysteine- or click-based), site-defined attachment can be used to improve conjugate homogeneity and analytical clarity.
Common targets include HRP, ALP/AMP systems, penicillinase, α-D-galactosidase, glucose-6-phosphate dehydrogenase, biotin–avidin systems, and other enzyme or protein immunoconjugates.
Identity is confirmed by mass spectrometry, and purity or conjugation profiles are assessed by chromatographic methods as appropriate. Documentation and release criteria can be aligned to the intended use and program stage.
Selected peer-reviewed references describing peptide–enzyme conjugation, enzyme labeling strategies, and bioconjugation principles relevant to analytical, diagnostic, and biosensor applications.
Bio-Synthesis peptides and bioconjugates have been used in peer-reviewed biochemical and analytical studies; specific vendor attribution varies by publication.
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