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Affinity Tags

Biotinylated & Affinity-Tagged Oligonucleotides

High-quality biotinylated oligonucleotides for streptavidin capture—plus custom design & synthesis (5′ biotin, TEG, internal Bio‑dT, dual biotin), desthiobiotin (reversible), DIG/DNP haptens, and photocleavable biotin with NHS/click coupling, assay‑specific QC, and scalability to ISO 13485 production.

Overview Biotinylated Oligos Desthiobiotin Oligos DIG/DNP Probes Photocleavable Biotin Related Services Speak to Scientist

Overview

Bio-Synthesis, Inc. designs and manufactures biotinylated oligonucleotides and affinity-tagged DNA/RNA for capture, immobilization, enrichment, and detection. Under our OligoDx™ platform we offer 5′ biotin oligos, biotin TEG spacers, biotinylated dT (internal), NHS-biotin for post-synthetic labeling, and click-ready azide options, along with desthiobiotin for reversible capture, digoxigenin (DIG) and DNP hapten probes, and photocleavable biotin for on-demand release.

Typical applications include streptavidin/NeutrAvidin capture on magnetic beads, ELONA/ELISA-like plates, microarrays & biosensors, ISH/DIG detection, lateral flow, and library prep. We provide HPLC purification, ESI-MS, and documentation aligned with ISO 9001/13485.

Our services include custom design & synthesis (5′ biotin, biotin TEG, internal Bio‑dT, dual biotin, desthiobiotin‑TEG, DIG/DNP, PC‑Biotin), conjugation via NHS‑ester or click chemistry, and assay‑specific QC (HPLC, ESI‑MS; streptavidin‑binding tests on request). We manufacture from research grade to ISO 13485 production and support related workflows such as enzyme‑labeled oligos, custom DNA/RNA probes, and diagnostic oligos for ELONA & lateral flow.

Services at a glance
  • Custom affinitized oligos: 5′ biotin, biotin TEG, internal Bio‑dT, dual biotin
  • Reversible capture: desthiobiotin (with TEG and azide options)
  • Hapten probes: DIG/DNP for antibody-based detection (ISH, ELISA-like)
  • Photocleavable biotin: UV-triggered release for library prep & bead workflows
  • Coupling chemistries: NHS-ester and azide/alkyne click
  • Analytical QC: HPLC, ESI‑MS; streptavidin-binding tests on request
  • Scale & documentation: research to ISO 9001/13485
Streptavidin Capture Reversible Elution Hapten Detection Click-Ready

Biotinylated Oligos (5′, Internal dT, TEG Spacers)

Primary keywords: biotinylated oligonucleotides, biotin-labeled oligos, 5′ biotin oligo, biotin TEG oligo, NHS-biotin oligo.

Hide products & notes
Product Function Application Code
Biotin deoxythymidine dT (internal) Internal biotin label via dT; high-affinity streptavidin/NeutrAvidin binding. Immobilization on beads/chips; pull-downs; capture of DNA/RNA probes. [Bio-dT]
Dual Biotin Two biotins increase avidity and retention under stringent wash or high-flow. Robust bead capture; lateral-flow test lines; surface tethering. [Bio-Dual]
Biotin NHS NHS-ester biotin for post-synthetic coupling to primary amines (5′-amine, amino-link). Flexible labeling of amine-modified oligos/primers; custom assembly. [Bio-N]
Biotin TEG (15-atom triethylene glycol spacer) 5′ 5′ biotin with long TEG spacer to reduce steric hindrance and improve binding. Microarrays, biosensors, ELONA capture, magnetic beads. [Bio-TEG-5]
Biotin-5′ Standard 5′ biotin label for strong streptavidin capture. General immobilization, pull-downs, lateral flow conjugates. [Bio-5]
Technical Notes
  • TEG spacer vs. 5′ biotin: TEG (PEG-like) increases accessibility on crowded surfaces; often improves binding kinetics.
  • NHS-biotin coupling: React at pH 7.5–8.5 in anhydrous buffer; avoid Tris. Quench and purify by HPLC.
  • Internal biotin (dT): Choose positions away from critical hybridization sites to avoid duplex disruption.

Desthiobiotin Oligos: Reversible Streptavidin Capture

Secondary keywords: desthiobiotin oligo, reversible biotin oligo, desthiobiotin TEG, streptavidin capture oligo

Show Products & Notes
Product Function Application Code
Desthiobiotin NHS Biotin analog enabling gentle, reversible elution with added biotin. Affinity purification with mild release; on-bead assays. [DesBio-N]
Desthiobiotin TEG Desthiobiotin plus TEG spacer for low-steric, reversible binding. Capture-and-release workflows; bead or chip cycling. [DesBio-TEG]
Desthiobiotin TEG Azide Reversible tag with terminal azide for CuAAC/SPAAC click attachment. Click-to-surface assembly; modular probe fabrication. [DesBioTEG-N3]
Technical Notes
  • Elution strategy: Elute desthiobiotin-bound oligos by competing with free biotin; preserve protein/complex integrity.
  • When to use: Prefer desthiobiotin for capture-release workflows; use dual biotin when maximum retention is required.

DIG/DNP Hapten-Labeled Oligos (Antibody Detection)

Secondary keywords: DIG-labeled oligo, digoxigenin-labeled DNA probe, DNP-labeled oligo, ISH probes.

Show Products & Notes
Product Function Application Code
Digoxigenin NHS Hapten recognized by anti-DIG antibodies; avoids endogenous biotin background. In situ hybridization (ISH), ELISA-like detection, multiplex immunodetection. [Dig-N]
DNP TEG (2,4-dinitrophenyl) Hapten detected by anti-DNP antibodies; TEG spacer improves accessibility. Immuno-PCR, multiplex bead assays, secondary confirmation labels. [DNP-TEG]
Technical Notes
  • DIG vs. biotin: Use DIG/DNP when endogenous biotin causes background or for multiplex with anti-hapten antibodies.
  • Signal chains: Anti-DIG or anti-DNP antibodies can be HRP/AP-conjugated for chemiluminescent readout.

Photocleavable Biotin (PC-Biotin) Oligos

Secondary keywords: photocleavable biotin oligo, PC-biotin oligo, UV-cleavable biotin release 365 nm

Show Products & Notes
Product Function Application Code
PC Biotin (photocleavable) UV-cleavable linker releases oligos from streptavidin on demand. Library prep and bead release; single-molecule workflows; on-bead synthesis. [PCBio]
Technical Notes
  • Typical cleavage at ~350–365 nm; verify intensity/exposure to avoid sample damage.
  • Minimize UV exposure for sensitive fluorophores or proteins; consider shielding non-target regions.

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Custom spacers, multi-tag designs, click chemistry, and assay-specific QC — from research to ISO 13485 production.

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