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Streptavidin–Oligonucleotide Conjugation Services

Site-defined protein–oligonucleotide conjugates for capture, immobilization, detection, and assay development.

Custom streptavidin conjugation supporting single-stranded DNA, double-stranded DNA, RNA, siRNA, ASO, SSO, and PNA across affinity purification, surface immobilization, molecular detection, and biotin–streptavidin assay workflows.

Request a Quote Supported oligo types Chemistry
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Overview Supported oligo types Why streptavidin Applications Conjugation chemistry Design & routes QC Contact

Overview

Bio-Synthesis provides custom streptavidin–oligonucleotide conjugation services for a wide range of nucleic acid formats, enabling chemically defined constructs for biotin-based capture, nucleic acid immobilization, probe assembly, molecular detection, and development-ready assay workflows.

Single-stranded DNA Double-stranded DNA RNA / siRNA ASO / SSO PNA Biotin capture systems Protein–oligonucleotide conjugation services

Streptavidin is a tetrameric biotin-binding protein widely used in biotechnology because of the exceptionally strong non-covalent interaction between biotin and streptavidin. This relationship supports highly stable molecular recognition systems for bead-based capture, plate or surface immobilization, barcoded probe design, and modular assay assembly.

Our platform supports streptavidin conjugation to single-stranded oligonucleotides, double-stranded constructs, siRNA duplexes, antisense oligonucleotides, splice-switching oligonucleotides, and peptide nucleic acids, with purification and analytical control aligned to the intended use and program stage.

Streptavidin–oligonucleotide conjugate architecture showing biotinylated oligonucleotides binding to streptavidin tetramer
Streptavidin–oligonucleotide conjugate architecture showing biotinylated DNA, siRNA, ASO, SSO, and PNA formats interacting with a streptavidin tetramer.

Related services: Biotinylated Oligonucleotides, Affinity Label Oligonucleotide Conjugation Services, Protein–Oligonucleotide Conjugates, Oligonucleotide Conjugation Services.

Why use streptavidin–oligonucleotide conjugates?

  • Extremely strong biotin–streptavidin binding supports stable capture and immobilization workflows
  • Tetrameric streptavidin architecture enables multivalent interaction designs
  • Well suited for magnetic bead capture, plate coating, microarray assembly, and modular detection systems
  • Compatible with DNA, RNA, siRNA, ASO, SSO, and PNA when appropriate handles and routes are selected
  • Useful for both research-grade builds and development-aware analytical workflows

Streptavidin-based constructs are especially attractive when the goal is to connect a defined nucleic acid sequence to a robust protein scaffold that can engage with biotinylated systems in a modular and reproducible way.

Supported oligonucleotide types

DNA constructs

Supports both single-stranded and double-stranded DNA constructs for capture and assay workflows.

  • Single-stranded DNA probes
  • Double-stranded DNA constructs
  • Barcoded and hybridization formats
RNA & siRNA

RNA and duplex siRNA formats can be adapted to streptavidin-based platforms with suitable handle design.

  • Single-stranded RNA
  • siRNA duplexes
  • Program-specific modified RNA formats
ASO / SSO / PNA

Supports therapeutic and probe-oriented oligonucleotide chemistries that benefit from defined protein attachment.

  • Antisense oligonucleotides (ASO)
  • Splice-switching oligonucleotides (SSO)
  • Peptide nucleic acids (PNA)
Compatibility note

Feasibility depends on the oligonucleotide handle, modification pattern, strand format, and the intended application. If you already have a biotinylated or chemically functionalized design, we can review the most practical route to a streptavidin-linked construct.

Common applications

Affinity purification & isolation

Biotin–streptavidin systems are widely used for nucleic acid and protein capture using beads, columns, and assay matrices.

  • DNA or RNA capture workflows
  • Complex isolation and pull-down studies
  • Probe-guided affinity enrichment
Immobilization & assay assembly

Streptavidin-linked oligos are useful in modular assay formats that require reliable surface or scaffold presentation.

  • Plate and surface immobilization
  • Microarray and hybridization layouts
  • DNA-directed assembly concepts
Detection & barcoding

Protein–oligonucleotide constructs can support molecular detection, analytical readouts, and coded assay designs.

  • Probe-based detection systems
  • Molecular barcoding approaches
  • Multiplexed research workflows

Linker & conjugation chemistry

Thiol–maleimide

Useful when cysteine or thiol-bearing components are part of the design strategy.

  • Fast coupling kinetics
  • Common for protein-directed builds
  • Requires thoughtful handle control
Amine-reactive routes

NHS-based chemistry can be useful when amine-accessible conjugation is the most practical route.

  • Lysine-accessible protein surfaces
  • Flexible but potentially less site-specific
  • Useful for selected assay builds
Click-compatible routes

Bioorthogonal handles may be used when more selective or modular conjugation is preferred.

  • Azide–alkyne strategies
  • DBCO / copper-free click options
  • Useful for modular design control
Route selection note

The best chemistry depends on streptavidin format, oligonucleotide handle, required stoichiometry, and the level of site control needed for the final construct.

Design inputs & build routes

Design inputs we review
  • Oligonucleotide type: ssDNA, dsDNA, RNA, siRNA, ASO, SSO, or PNA
  • Handle strategy: biotin, thiol, amine, azide, alkyne, or project-specific alternatives
  • Strand format: single-stranded vs duplex or more complex assemblies
  • Intended use: capture, immobilization, detection, purification, or barcoding
  • Purification depth: aligned to feasibility, research, or development-stage needs
Typical build routes
  • Direct chemical conjugation to streptavidin using compatible functional handles
  • Biotin-mediated assembly concepts in matched streptavidin / biotin system designs
  • Modular assay-oriented architectures for bead, plate, array, or detection workflows
  • Program-specific optimization for stoichiometry, steric accessibility, and assay performance
System relationship: biotin and streptavidin

Because biotin and streptavidin function as a matched high-affinity binding system, streptavidin–oligonucleotide conjugates are often developed alongside biotinylated DNA, RNA, siRNA, ASO, SSO, and PNA constructs for capture, detection, immobilization, and modular assay workflows.

QC & typical deliverables

Standard QC
  • Purity assessment using chromatography-based methods where applicable
  • Identity or conjugation confirmation using orthogonal analytical methods
  • COA and method summary aligned to intended use
Optional controls
  • Matched unconjugated oligo controls
  • Biotinylated comparison materials
  • Route or linker comparison variants
Aligned to program stage

Analytical depth and documentation can be scaled for exploratory builds, assay development, or more advanced translational workflows.

Our Quality Commitment

Bio-Synthesis applies controlled production workflows, in-process review, and final analytical verification to support high-quality streptavidin–oligonucleotide conjugates suitable for reproducible research and development programs.

Identity confirmation, purity review, and documentation are aligned to the construct design and intended use. Our quality systems support consistent materials, traceability, and customer-focused execution across broader oligonucleotide and bioconjugation services.

FAQ

What oligonucleotide types can be conjugated to streptavidin?

We support streptavidin conjugation to single-stranded DNA, double-stranded DNA, RNA, siRNA, ASO, SSO, and PNA, subject to handle and design compatibility.

Why use streptavidin in oligonucleotide conjugation?

Streptavidin is widely used because it binds biotin with extremely high affinity and provides a stable protein scaffold for capture, immobilization, detection, and modular assay assembly.

Can streptavidin–oligonucleotide conjugates be used with biotinylated systems?

Yes. Streptavidin–oligonucleotide conjugates are often developed alongside biotinylated oligonucleotides for affinity purification, surface immobilization, molecular detection, and bead-based capture workflows.

What do you need for a quote?

Please provide oligonucleotide type, sequence and modification pattern, single- or double-stranded format, desired attachment site or functional handle, preferred linker or chemistry if known, target quantity, purity requirements, and intended application.

More FAQ'sAll FAQ's

Contact & quote request

For the fastest quote, send your oligonucleotide type and sequence, strand format, any existing modification pattern, desired handle or attachment preference, preferred chemistry if known, and quantity / purity targets.

Fast quote checklist
  • Oligonucleotide class (DNA, dsDNA, RNA, siRNA, ASO, SSO, or PNA)
  • Sequence and modification pattern
  • Single-stranded or duplex / double-stranded format
  • Functional handle or desired attachment strategy
  • Preferred chemistry or request for recommendation
  • Quantity, purity target, and intended application

Not sure which route fits your build? Send the sequence, chemistry, and application constraints—we’ll recommend a practical design path.

Fastest path
Request a QuoteSupported oligo types

Recommended Reading

References that cover streptavidin–biotin systems, protein–oligonucleotide conjugation, and affinity-based nucleic acid workflows.

  • Green NM. Avidin and streptavidin. Methods Enzymol.
  • Langer PR, Waldrop AA, Ward DC. Enzymatic synthesis of biotin-labeled polynucleotides. Proc Natl Acad Sci USA.
  • Leary JJ, Brigati DJ, Ward DC. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes. Proc Natl Acad Sci USA.
  • Winkler J. Oligonucleotide conjugates for therapeutic applications. Ther Deliv.
  • Klabenkova K, Fokina A, Stetsenko D. Chemistry of peptide-oligonucleotide conjugates: a review. Molecules.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

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