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Precision-Engineered Multivalency for Next-Gen Oligo Delivery

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Custom Dendrimer Oligonucleotide Synthesis

Nuclease‑resistant closed‑circular DNA/RNA for padlock probes, RCA, and advanced molecular assays.

Overview Technology Synthesis Options Supported Cores Linkers & Chemistries FAQ Contact

Overview

Dendrimers are monodisperse, highly branched macromolecules with a programmable number of surface end‑groups. When conjugated to oligonucleotides, they enable multivalent display, tunable size/charge, and modular surface chemistry for delivery, imaging, and capture workflows.

Bio‑Synthesis offers end‑to‑end dendrimer–oligonucleotide synthesis across PAMAM, PPI, carbosilane, and lysine scaffolds with controlled average valency, advanced linkers, and documented quality control.

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At-a-Glance

  • Cores: PAMAM, PPI, carbosilane, lysine
  • Generations: G0–G6+ (application‑dependent)
  • Valency: low (≤4) to high (≥64) with reporting
  • Oligos: DNA, RNA, ASO, siRNA; labels and handles
  • QC: MS/UV‑Vis, HPLC/UPLC; optional DLS/zeta
  • Scales: mg to gram quantities; bulk on request

Supported Cores & Attributes

Core Family Typical End‑Groups Attributes Common Uses
PAMAM –NH2, –OH, –COOH, acetylated, guanidinium Well‑characterized; G0–G7+; high uniformity ASO/siRNA delivery, imaging, capture
PPI –NH2, quaternary ammonium More compact vs PAMAM at same generation Condensation, delivery, sensors
Carbosilane Functional silanes, Si‑alkyl/aryl Hydrophobic skeleton; robust tailoring Gene delivery, coatings, probes
Lysine‑based –NH2, orthogonal protected handles Biogenic backbone; bio‑linking versatility Bioconjugation, multivalent displays

We recommend the core/generation based on application, charge tolerance, and desired valency.

Generations & Approximate Terminal Groups (PAMAM)

Generation Nominal Terminal Groups Notes
G0 4 Smallest fully built generation
G1 8 Size and charge increase vs G0
G2 16 Useful for low‑valency displays
G3 32 Balanced size/valency
G4 64 High multivalency for dense loading
G5 128 Very high loading—consider shielding
G6 256 Large; evaluate cytotoxicity & charge

Actual substitution depends on chemistry and reaction conditions; we report the measured average valency per batch.

Custom Synthesis Options

Parameter Options
Oligo Type DNA, RNA, ASO, siRNA; mixed constructs
Valency Targeted average (reported per lot)
Labels/Tags Dyes (FAM/Cy), biotin, digoxigenin
Reactive Handles Amine, thiol, azide/alkyne, maleimide
Purification HPLC/UPLC (standard); SEC optional
Formulation Lyophilized or buffer; custom aliquots
Scales mg to gram; bulk/custom on request
Documentation CoA, valency calculation, analytical data

Linkers & Conjugation Chemistries

Oligo Handles
  • 5′/3′‑amines (primary/secondary), 5′‑thiol (protected), 5′‑azide/alkyne
  • 5' Trebler, long trebler, symmetric doubler, asymmetric doubler (Lev)
  • Biotin, dyes (FAM, Cy3/Cy5), quenchers for tracking
  • Phosphorothioate or modified backbones on request
Conjugation Strategies
  • NHS‑ester ⇄ amine; EDC/NHS ⇄ carboxyl
  • Maleimide ⇄ thiol; SMCC/SPDP heterobifunctionals
  • CuAAC/SPAAC click; thiol–ene photochemistry
Spacers & Stabilizers
  • PEG(n), AEEA/Ahx for spacing and solubility
  • Cleavables: disulfide, photocleavable spacers
  • Charge/steric tuning via mixed end‑groups

How It Works

1) Scope & Design

Define core/generation, oligo type/length, target valency, and labels. We review feasibility and chemistry.

2) Build & Verify

Conjugation, purification, and analytics (HPLC/MS; valency report). Optional additional tests as needed.

3) Deliver & Support

Ship with documentation; ongoing support for optimization, scale‑up, or additional modifications.

Dendrimer–Oligonucleotide Technology & Benefits

Overview: Dendrimer–oligonucleotide conjugates combine the precise, multivalent architecture of dendrimers with the specificity of DNA/RNA sequences. This hybrid platform enables enhanced delivery, high payload capacity, and modular functionalization for applications in research, diagnostics, and therapeutics.

Technology

  • Core architecture: PAMAM, PPI, carbosilane, or lysine dendrimers with tunable generations and end‑group counts.
  • Valency control: Precisely defined number of oligos per dendrimer, verified analytically.
  • Linker diversity: NHS–ester/amine, maleimide/thiol, click chemistries, PEG spacers, cleavable linkers.
  • Payload flexibility: DNA, RNA, ASO, siRNA; compatible with dyes, biotin, and targeting ligands.
  • Surface tailoring: Charge, hydrophobicity, and targeting tuned for application‑specific needs.

Benefits

  • High loading capacity — multiple oligos per dendrimer boost local concentration.
  • Enhanced stability — multivalent display protects oligos from degradation.
  • Improved delivery — surface chemistry optimized for cell/tissue uptake.
  • Versatile functionalization — integrate imaging labels, affinity tags, or ligands.
  • Reproducibility — monodisperse dendrimers yield consistent quality.
Discuss My Dendrimer–Oligo Project

FAQ

Which core should I choose?

PAMAM is versatile and widely characterized; PPI is more compact; carbosilane offers hydrophobic skeletons; lysine‑based provides biogenic backbones. We’ll recommend based on your application and target valency.

What valency can you achieve?

From low (≤4) to high (≥64) average attachments per particle depending on generation, chemistry, and sterics. We report the measured average per batch.

Can you add dyes or ligands?

Yes—fluorophores (FAM/Cy), biotin/digoxigenin, and custom ligands can be co‑displayed with oligos using orthogonal linkers.

Do you offer endotoxin/sterility testing?

Optional LAL endotoxin and sterility testing are available for sensitive applications.

Ready to design your dendrimer–oligo conjugate?

Tell us your core, generation, oligo type, and target valency—our team will send a tailored plan and quote.

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