From Scouting to Validation — Complete Analytical Support for Oligos.

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Analytical Method Development and Validation

Scouting → optimization → transfer → ICH Q2(R2) validation for DNA/RNA oligonucleotides. Identity, purity/impurities, residuals, elemental impurities, and general attributes with audit‑ready documentation.

ISO 9001:2015 ISO 13485:2016 ICH Q2 (R2) Methods GLP / cGMP Aligned

Overview

End‑to‑end oligo analytical method support—from scouting and optimization to client method transfer, verification, and full ICH Q2(R2) validation. We generate audit‑ready protocols, raw data, and summarized validation reports.

Assays span identity (LC–MS/MALDI), purity/impurities (HPLC/CE/IEX), residual solvents (GC), elemental impurities (ICP‑MS), and general attributes (KF, pH, optional sodium content).

Discuss Your Method

At-a-Glance

  • Lifecycle: Scouting → Optimization → Transfer/Verification → Validation
  • Validation: Accuracy, precision, specificity, linearity/range, LoD/LoQ, robustness
  • Identity: LC–MS/MALDI; optional enzymatic mapping
  • Purity/Impurities: RP/IP‑RP‑HPLC, CE; IEX for charge variants
  • Residuals/Elements: GC (solvents), ICP‑MS (metals)
  • General: KF, pH, sodium content (IC or flame photometry)

Assay Capabilities

  • Identity: LC–MS (ESI) or MALDI–TOF; optional enzymatic digest mapping; LC‑MS/MS for sequence mapping
  • Purity / Impurities: RP‑/IP‑RP‑HPLC, CE; IEX for charge variants; track shortmers, n‑1/n‑2, depurination, conjugate loss
  • Residuals: GC for solvents; ICP‑MS for elemental impurities; optional sodium content trending (ion chromatography or flame photometry)
  • General attributes: Karl Fischer water, pH, appearance; endotoxin/bioburden as required

Method Lifecycle

  • Scouting: column/mobile phase screens, detection mode, gradient & temperature windows
  • Optimization: resolution & peak shape, carryover, specificity, stress‑challenge for stability indication
  • Transfer & Verification: client method transfer, system suitability, analyst/equipment checks
  • Validation: ICH Q2(R2) protocol, execution, and report with acceptance criteria and raw data

Validation Characteristics Matrix

Characteristic Identity (LC–MS) Purity (HPLC/CE/IEX) Residuals (GC) Elemental (ICP‑MS) KF / pH / Sodium
Specificity / Selectivity
Accuracy
Precision (Repeat./Intermediate)
Linearity & Range
LoD / LoQ
Robustness
System Suitability

Example matrix — finalized per assay and intended use. "KF / pH / Sodium" covers general attributes including Karl Fischer water, pH, and optional sodium content trending.

Method Transfer & Change Control

  • Client method transfer with verification and readiness checks
  • Instrument qualification references and maintenance logs
  • Change control (versions, deviations, CAPA as applicable)
  • Training records and analyst proficiency tracking

Sample Requirements

  • Typical development: ≥ 300–500 µg per method iteration (assay‑dependent)
  • Validation: additional aliquots for accuracy/precision/robustness runs
  • Dry or buffered format; include length, chemistry, purification, concentration
  • Shipping: ambient for DNA; cold chain recommended for RNA/ASO

Instrumentation

  • HPLC/UPLC (UV/FL/MS), CE, IEX
  • LC–MS / MALDI (intact mass); LC‑MS/MS (mapping)
  • GC (headspace for volatiles), ICP‑MS (metals)
  • KF titration, pH, osmolality; ion chromatography/flame photometry for sodium

Documentation & Deliverables

  • Protocols and validation plans with acceptance criteria
  • Method versions, change logs, deviations, and CAPA (as applicable)
  • Raw/processed data, chromatograms/spectra, calculations
  • Summary validation report and transfer/verification report
Release

Oligonucleotide Release
QC

Identity, purity/impurities, residuals, sodium content, and safety testing with signed CoA.

Explore Release QC
Stability

Oligonucleotide Stability Studies

Accelerated/intermediate/long‑term programs with stability‑indicating methods and sodium trending.

See Stability Studies
Manufacturing

Custom Oligonucleotide Synthesis

RUO to GMP‑aligned oligos; HPLC/PAGE, diafiltration; broad chemistry & conjugation support.

Explore Synthesis

Already have a draft method?

We can transfer and verify it, then design an ICH Q2(R2) validation plan around your intended use

Start a Method Plan

Discuss Your Method

Tell us about your assay and intended use — we’ll return a phased plan with timelines and documentation deliverables.

Full Name *
Email *
Company / Institution *
Phone *
Intended Use
Existing Method?
Validation Needed?
Assay Type
Notes (sequence chemistry, target specs, matrices, special attributes to trend)

By submitting, you agree to be contacted regarding your request.

FAQ

Which assays can you develop and validate?

Identity, purity/impurities, residual solvents, elemental impurities, Karl Fischer water, pH/osmolality, and optional sodium content trending.

How do you structure a validation?

We finalize a protocol with acceptance criteria, then execute accuracy, precision, specificity, linearity/range, LoD/LoQ, robustness, and system‑suitability runs as required by ICH Q2(R2).

Do you support method transfer from my lab?

Yes. We perform transfer with verification, instrument suitability checks, and documentation, then proceed to validation if requested.

What sample amount is required?

Development typically needs ≥ 300–500 µg per iteration; validation requires additional aliquots for accuracy/precision/robustness runs.

Contact

Email: info@biosyn.com • Phone: +1 (972) 420‑8505

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