Custom epitope, long-peptide, peptide pool, and neoantigen synthesis for immunology research — from mg-scale screening to multi-gram and large-scale projects (up to 100 g+), with defined specifications and documented analytical verification.
Vaccine peptides are synthetic peptide antigens used in immunology research for epitope mapping, immune stimulation assays, peptide pool screening, and neoantigen evaluation. [1], [2]
In preclinical immunology, peptides are used for epitope mapping, T-cell stimulation (e.g., ELISpot/ICS), antibody binding studies, and formulation feasibility. Many programs also use long peptides and peptide pools to broaden immunogenic coverage and support screening.
Epitope selection and validation commonly reference curated immune databases such as the Immune Epitope Database (IEDB) and related NIAID resources, which catalog experimentally validated T-cell and B-cell epitopes. [3]
Overlapping peptide libraries (e.g., 15-mers with defined overlap) are widely used to map antigenic regions and broaden coverage in ELISpot and intracellular cytokine staining (ICS) workflows. [4]
Note: We synthesize vaccine peptides for research use by customer-provided sequence(s) and project-defined specifications.
Define MHC class I/II epitopes, stimulate antigen-specific T cells, and compare variants.
Learn more about T-cell peptide libraries →
Overlapping peptide sets or curated epitope pools for broad antigen coverage.
Explore peptide pool screening →
Custom mutation-derived peptides and long-peptide formats for cancer vaccine studies.
See neoantigen peptide →
Linear epitope peptides for binding assays, controls, and method development.
Evaluate stability, solubility, and delivery constraints using defined peptide constructs.
Assess cross-reactivity and immune recognition across strains or sequence variants.
Typically short peptides used in antigen-specific T-cell stimulation assays.
Longer sequences used in processing/presentation studies and multi-epitope designs.
Overlapping tiles or curated epitope pools for screening and immunogenicity profiling.
If you already have IEDB IDs, UniProt positions, or immunoinformatics outputs, include them with your quote request for faster review. [1]
Project-defined MHC class I and class II epitope peptides for stimulation assays (e.g., ELISpot/ICS), mapping, and benchmarking.
Overlapping peptide sets and curated pools to broaden coverage and support efficient screening, including pool/subpool formats.
Custom mutation-derived peptides, long-peptide formats, and matched controls for personalized vaccine and immunogenicity studies.
Variant-aware epitope panels and comparative sets to study cross-reactivity, conservation, and strain-to-strain differences.
Linear epitope peptides for binding assays, method development, and control reagents.
Truncations, alanine scans, positional variants, and project-defined control sets to support SAR and reproducibility.
FASTA, CSV, or Excel. For pools/libraries, include peptide length, overlap, and target region coordinates (if applicable).
If you’re pulling sequences from IEDB, include IEDB IDs or exported lists and any supporting context. [1] For background on the IEDB resource, see NIAID’s overview. [4]
Below are representative research targets frequently used in immunology studies. We synthesize peptides based on your specified sequence(s), length, and intended use.
If you share the pathogen/protein target, HLA context (if known), and assay type, we can help you structure a practical set (single epitopes, variants, or pools) based on your objectives. IEDB is commonly used as a discovery and validation resource. [1,4]
Below is a practical summary of what we routinely support for vaccine peptide programs. If your program has non-standard requirements, include them in the quote request so we can confirm feasibility and recommended QC.
FASTA/CSV/Excel lists are accepted. If available, include IEDB IDs/exports or UniProt positions to speed review. [1]
Share target, assay type (ELISpot/ICS/binding), and any HLA context. We can help structure epitopes vs pools vs long-peptides. IEDB and NIAID resources are commonly used for epitope selection workflows. [1,4]
Terminal caps, linkers/handles, conjugation-ready designs, and stability-oriented substitutions can be combined as requested (sequence-dependent).
Analytical HPLC/UPLC purity profile and LC-MS intact mass confirmation (when feasible).
Project-defined documentation package (e.g., CoA, synthesis notes, handling guidance).
Matched controls, variants, and pool compositions to support reproducibility.
Project-defined confirmation steps can be applied at the individual peptide level prior to pooling (e.g., LC-MS/HPLC checks where feasible).
Defined lists, traceable handling, and pool/subpool design choices help reduce ambiguity and support reproducibility.
If your workflow benefits, we can discuss using marker peptides or a verification plan tailored to your pool design and assay sensitivity.
Pool QC approaches depend on pool size, peptide properties, and intended assay. Include your intended pool format and acceptance criteria in your quote request.
Stable isotope labeling for LC–MS/MS quantification and standards.
Explore more about Isotope-Labeled Peptides
Biotinylation, lipidation, PEGylation, and functional conjugates.
Explore more about Peptide Conjugation
Stability-oriented residue substitutions and advanced designs.
Learn more about Unnatural AAs Learn More about D-amino Acids
A synthetic peptide antigen (often an epitope) used in immunology research to study or elicit specific immune responses. Epitope selection and validation commonly reference IEDB. [1]
Epitope peptides are typically short sequences used directly in stimulation assays, while long peptides are designed for processing/presentation workflows and may contain multiple epitopes.
Many screening workflows use crude or ≥95% depending on assay sensitivity and peptide properties. Confirmatory work commonly specifies ≥95% or ≥98%. Define your acceptance criteria in the quote request.
A pool built from overlapping peptides that tile across a protein/region to broaden coverage and support epitope mapping and screening.
A common approach is to generate 15-aa peptides that start every 5 residues across the target region (15 length minus 10 overlap = 5 step). Share the target sequence/region and we can help format the list.
Yes. We synthesize single epitopes, overlapping peptide sets, curated pools/subpools, and custom neoantigen peptides (including long-peptide formats) as requested.
Typical deliverables include analytical HPLC/UPLC purity profile and LC-MS intact mass confirmation (when feasible), plus documentation (e.g., CoA).
FASTA/CSV/Excel lists are accepted. If available, include IEDB exports/IDs, UniProt positions, and any HLA context to speed review. [1,4]
If you’re unsure which sequences to order, share the target (pathogen/protein/tumor antigen), desired coverage (epitopes vs pools), and any constraints (HLA context, variants, peptide length). We’ll help structure a practical build plan.
Email: sales@biosyn.com Phone: +1 (800) 227-0627 | + (972) 420-8505
References are provided for scientific background on epitope discovery, immune mapping, and peptide-based vaccine research (for context only; not clinical claims).
E-E-A-T note: References are included for background on immune epitope mapping and peptide-based research workflows and do not imply clinical or therapeutic claims. Our services support custom peptide synthesis for research use.
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