Peptide–engineered recombinant protein conjugation is the covalent attachment of synthetic peptides to
sequence-defined recombinant proteins that are intentionally designed to support
controlled chemical modification. In contrast to traditional carrier protein formats,
engineered recombinant scaffolds enable the rational placement of conjugation handles and reduction of
competing reactive residues, improving homogeneity and reproducibility
[1], [2].
Protein engineering strategies such as the introduction of site-specific residues, genetically encoded
ligation motifs, or orthogonal functional groups allow peptide attachment with
defined stoichiometry and predictable geometry. These approaches are widely used to
generate homogeneous bioconjugates for functional, analytical, and mechanistic studies where batch-to-batch
consistency is critical
[1],
[3].
This service focuses on recombinant protein scaffolds that are intentionally engineered to enable
site-specific handles, defined stoichiometry, and
homogeneous conjugates. When required, Bio-Synthesis integrates
molecular biology → recombinant protein expression → purification → peptide conjugation → QC
under a single workflow, aligning construct design with downstream conjugation chemistry and analytical
requirements
[2], [4].
Bio-synthesis (peptides + proteins)
Engineered recombinant proteins
Site-specific conjugation
Defined stoichiometry
Homogeneous conjugates
Integrated expression & QC
Beyond peptide synthesis and conjugation, our bio-synthesis capabilities include
recombinant protein expression services to produce engineered protein scaffolds that are
“built for conjugation.” This integrated approach supports rational design decisions that preserve protein
function, minimize heterogeneity, and simplify characterization
[3], [5].
Figure: Engineered workflow for peptide–recombinant protein conjugation showing protein design, expression, purification, site-specific attachment, and QC.
Conjugation chemistry, handle selection, and characterization depth are selected on a
project-by-project basis based on peptide composition, protein scaffold design, and intended downstream use.