Long & High-Complexity Peptide Synthesis

A typical COA includes identity confirmation (MS) and purity profiling (HPLC). Additional testing (e.g., counter-ion analysis or composition checks) can be added upon request.

We start by reviewing sequence behavior and any prior data (HPLC/MS, crude purity, solubility notes). Common root causes include on-resin aggregation and reduced chain mobility. We then select the most reliable route—optimized SPPS, fragment-based assembly with ligation chemistry, or enzymatic synthesis (expression-based)—and align QC endpoints to your application.

Long peptides are commonly considered >50 amino acids. However, difficulty is driven more by sequence behavior (aggregation, hydrophobicity, repeats, disulfide density) than length alone.

Ligation chemistry (e.g., native chemical ligation) is typically used when the sequence is severely aggregation-prone, includes challenging motifs, or when a single continuous SPPS route is unlikely to meet targets.

Yes. For very long targets, peptide–protein hybrids, or domain-like constructs, expression or hybrid strategies can be more reliable than purely chemical synthesis. We’ll recommend the best route based on your requirements.

Purity is method-dependent and can be limited by chromatographic resolution and intrinsic heterogeneity. Share your application and we’ll recommend practical acceptance criteria and QC endpoints.

Yes. If you share prior HPLC/MS traces, crude purity estimates, or notes about solubility/aggregation, we can recommend a targeted rescue strategy (optimized SPPS vs fragments + ligation vs expression).

Timelines depend on sequence length, complexity, and synthesis route. Optimized single-chain SPPS projects typically require several weeks, while fragment-based assembly, ligation chemistry, or enzymatic synthesis routes may require additional time for fragment preparation, assembly, purification, and analytical verification. Project timelines are confirmed after sequence review.