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Enzyme Conjugation

Enzyme Oligo Conjugates

Custom enzyme–oligonucleotide conjugates—including HRP, AP, β-Gal and GOx—engineered for ELONA, qPCR, RCA/LAMP, lateral flow, and electrochemical biosensors. Scalable from research to ISO 13485 and cGMP-aligned production with full analytical QC.

Overview Reporter Enzymes Redox & Electrochemical Nuclease & Cleavage Polymerase & Ligase Speak to Scientist

Overview

Bio-Synthesis, Inc. provides comprehensive enzyme–oligonucleotide conjugation services under our OligoDx™ platform. We covalently attach enzymes such as horseradish peroxidase (HRP), alkaline phosphatase (AP), β-galactosidase, glucose oxidase (GOx), DNase I, polymerases, and ligases to custom DNA/RNA probes to enable signal amplification, chemiluminescent detection, electrochemical biosensing, molecular diagnostics, and sequencing workflows.

We offer custom linker strategies (PEG spacers, alkyl and cleavable linkers, click-chemistry handles) with site-specific 5′/3′ or internal labeling, backed by rigorous analytical QC (HPLC, ESI-MS/MALDI, SEC, activity assays). Synthesis scales from research grade through ISO 9001/13485 and cGMP-aligned production for application-ready, high-quality enzyme–oligo conjugates suited to ELONA, qPCR, rolling-circle amplification (RCA), LAMP, padlock ligation, lateral flow, and custom biosensors.

Signal Amplification Chemiluminescence Electrochemistry Isothermal Amplification

biotinylated capture oligos (streptavidin/NeutrAvidin) • custom DNA/RNA probes for hybridization & qPCR • diagnostic oligos for ELONA & lateral flow

Reporter Enzyme Conjugates (HRP, AP, β-Gal)

Chromogenic or chemiluminescent reporters for hybridization assays, blotting, and aptamer probes.

Hide Products & Notes
Enzyme Category Function Applications Code
Horseradish Peroxidase (HRP) Reporter Enzyme Conjugates Redox enzyme for chromogenic or chemiluminescent signal ELONA, blotting, aptamer probes [HRP]
Alkaline Phosphatase (AP) Reporter Enzyme Conjugates Chromogenic signal via phosphate cleavage Dot blots, in situ hybridization [AP]
β-Galactosidase Reporter Enzyme Conjugates Converts colorless substrates to detectable signal Hybridization assays, signal amplification [β-Gal]
Hide Technical Notes
  • Pair HRP with luminol/peroxide for ultra-sensitive chemiluminescence; AP with CDP-Star for low background.
  • Spacer length (PEG4–PEG24) reduces sterics and improves both activity and hybridization.
  • Confirm substrate compatibility if using PS/PN backbones or densely labeled probes.

Redox & Electrochemical Enzyme Conjugates (GOx, Lactate Oxidase, Urease)

Enzymes that generate electroactive species or pH shifts for amperometric, potentiometric, or colorimetric detection.

Show Products & Notes
Enzyme Category Function Applications Code
Glucose Oxidase (GOx) Redox & Electrochemical Enzyme Conjugates Redox catalysis for electrochemical detection Aptamer-based biosensors, lateral flow [GOx]
Lactate Oxidase Redox & Electrochemical Enzyme Conjugates Lactate oxidation for metabolite detection Metabolite biosensors [LOx]
Urease Redox & Electrochemical Enzyme Conjugates pH-based signal generation Point-of-care diagnostics [Urease]
Technical Notes
  • Use redox mediators (ferricyanide, ferrocene) or direct-wire to electrodes for fast readout.
  • pH-sensitive dyes (for urease) enable instrument-free colorimetric tests and paper diagnostics.
  • Check oxygen dependence (e.g., GOx) and buffer composition for stable signals.

Nuclease & Cleavage Enzyme Conjugates (DNase I, Nickase, ExoIII)

Sequence-programmed cleavage and nicking for probing, logic circuits, and amplification strategies.

Show Products & Notes
Enzyme Category Function Applications Code
DNase I Nuclease and Cleavage Enzyme Conjugates Random digestion of DNA Structure probing, enzymatic cleavage assays [DNaseI]
Nickase (Nb.BbvCI) Nuclease and Cleavage Enzyme Conjugates Site-specific nicking Strand displacement, DNA amplification [Nickase]
Exonuclease III Nuclease and Cleavage Enzyme Conjugates 3′→5′ exonucleolytic activity Signal-on detection strategies [ExoIII]
Technical Notes
  • Place nickase sites to trigger strand displacement (entropy probes, molecular switches).
  • Validate compatibility with modified backbones (PS/PN) and terminal labels.
  • Control temperature/ionic strength to tune cleavage rates and specificity.

Polymerase & Ligase Enzyme Conjugates (Taq, Reverse Transcriptase, T4 DNA Ligase)

Catalyze DNA extension or joining for rolling-circle, padlock, and isothermal detection formats.

Show Products & Notes
Enzyme Category Function Applications Code
Taq DNA Polymerase Polymerase & Ligase Enzyme Conjugates DNA strand extension RCA, LAMP, qPCR probes [Taq]
Reverse Transcriptase Polymerase & Ligase Enzyme Conjugates cDNA synthesis from RNA templates RT-PCR, detection systems [RT]
T4 DNA Ligase Polymerase & Ligase Enzyme Conjugates DNA ligation Padlock probes, ligation-based assays [T4Lig]
Technical Notes
  • For padlock probes, optimize gap/overlap and ligation temperature to reduce mismatch ligation.
  • Choose spacers that keep the enzyme active but preserve hybridization (PEG12–PEG24 often ideal).
  • Confirm Mg2+/salt conditions with modified bases or backbones to avoid activity loss.

Can’t find the enzyme conjugate you need?

We routinely source specialty enzymes, tailor linkers, and validate activity for your assay format.

Speak to a Scientist Browse All Modifications

Design & QC

Chemistry

  • Conjugation handles: primary amine, thiol, azide/alkyne; site-specific labeling on 5′/3′/internal.
  • Spacers: PEG (4–24), alkyl, cleavable linkers; maleimide, NHS-ester, click chemistry.
  • Backbones: PO/PS/PN as required by assay; verify enzyme compatibility.

QC & Documentation

  • Identity by ESI-MS; purity by HPLC; optional SEC for aggregates.
  • Functional testing by model substrates or activity probes on request.
  • COA with yield (OD/µmol), coupling ratios, and storage guidance.

FAQ

What’s the difference between enzyme conjugates and chemiluminescent detection?

Enzyme conjugates (e.g., HRP/AP) are the catalytic labels; chemiluminescent substrates convert that activity into light for high-sensitivity readout.

How do I choose between HRP and AP?

Use HRP for fast, ultra-sensitive chemiluminescence (luminol). Use AP with dioxetane substrates (e.g., CDP-Star) for low background or longer kinetics.

Which spacer length works best?

PEG/TEG spacers (PEG4–PEG24) reduce steric hindrance and often improve hybridization and enzyme access—start with PEG12 and tune by assay.

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