Rapid. Reliable. Secure
Stable, charge‑neutral antisense oligos tailored for splice‑ and translation‑blocking in cells and embryos.
Morpholino (PMO) antisense oligonucleotides replace the ribose–phosphate backbone with morpholine rings linked by phosphorodiamidate bonds, yielding a charge‑neutral, nuclease‑resistant chemistry. PMOs act by steric blocking—they do not recruit RNase H—making them ideal for splice modulation and translation blocking.
Bio‑Synthesis provides end‑to‑end custom PMO synthesis with labeling, linkers, and delivery enhancers, supported by rigorous QC documentation and optional advanced testing.
Highly resistant to nucleases and proteases; robust in serum and in vivo.
Minimizes non‑specific interactions and enables efficient hybridization under physiological conditions.
Steric‑block mechanism suits splice correction and translation start‑site blocking.
Standard labeled PMOs: 2–3 weeks from order confirmation. Complex chimeras or peptide conjugations may require additional time.
Lead time depends on sequence and modification complexity; rush options may be available.
Include organism/cell line and delivery method (e.g., microinjection, electroporation, CPP) if known.
Overview: Morpholino (PMO) and Thiomorpholino (TMO) oligonucleotides are single-stranded antisense chemistries that bind RNA with high specificity to sterically block processes such as translation initiation or pre-mRNA splicing. Both are engineered for exceptional nuclease resistance and reliable performance in complex biological matrices.
Performance depends on sequence, accessibility, and delivery. We can optimize chemistry and conjugation for your target tissue and assay format.
PMOs bind their complementary RNA by base pairing and sterically block the ribosome (translation‑blocking) or spliceosome (splice‑switching). They do not recruit RNase H and therefore do not cleave the RNA.
Most designs are 20–25 bases. Shorter (~18–20) can increase specificity for clamping; longer may be used for challenging targets. We can optimize based on your target and delivery method.
For splice modulation, target the exon–intron junction (donor/acceptor) or regulatory elements. For translation blocking, target the AUG/start site and flanking UTR. Avoid long homopolymer runs and extreme GC.
Common options include microinjection (embryos), electroporation, CPP conjugation (arginine/guanidinium‑rich peptides), and lipid conjugation. We can recommend strategies for your model.
Each lot includes MS identity confirmation and an analytical HPLC chromatogram. Additional functional/hybridization tests can be added.
Store lyophilized PMOs at 4 °C (short‑term) or −20 °C (long‑term). In solution, use nuclease‑free water or buffer (pH 7–8), aliquot, and keep at −20 °C to minimize freeze‑thaw.
Yes. We support RUO through GLP and cGMP with appropriate documentation and QA oversight. Include regulatory requirements in your request.
Complete the form to receive a tailored quote. Your request will be emailed to info@biosyn.com and logged to your CRM endpoint (configure below).
By submitting, you agree to be contacted regarding your request.
Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.