Custom cross-linker oligonucleotides—psoralen intercalators and halogenated bases—engineered for UV-induced covalent bonding in DNA/RNA. Designed to probe nucleic acid secondary structures and stabilize protein–nucleic acid complexes.
UV-activated cross-linking chemistries enable covalent bonds between DNA/RNA strands or between nucleic acids and proteins. These modifications are essential for structural biology, probing nucleic acid secondary structures, and mapping protein–nucleic acid interactions.
Cross-linkers fall into two categories: nucleic acid intercalators such as psoralen for site-specific targeting in double- and triple-stranded DNA, and halogenated bases like 5-bromo-dC for intra-strand or protein-directed cross-linking.
Bio-Synthesis provides custom cross-linker oligonucleotides tailored for research, diagnostics, and therapeutic applications, with scalable synthesis and full QC support.
Representative cross-linker modifications available for custom synthesis.
We can model Tm and suggest positions to balance cross-link yield with hybridization and off-target risk.
We can provide small-scale pilot lots for method optimization prior to scale-up.
Purification via HPLC/PAGE per construct; conjugates may use RP-HPLC with diafiltration.
We can align to internal SKUs and report extinction coefficients for modified bases.
Duplex stabilization with cross-linker placement guidance.
Gapmer & steric-block designs with cross-linker options.
Modified bases, long RNA, capping, poly(A).
Our team can recommend optimal modification sites, UV conditions, and QC strategies for your project.
Psoralen intercalators are preferred for site-specific interstrand links in double- or triple-helical regions. Halogenated bases (e.g., 5-Br-dC, 5-I-dU) are often chosen for intra-strand links or direct protein capture.
Yes. Cross-linkers are compatible with a wide range of conjugations; we’ll advise on placement and purification to avoid interference.
Effects are context-dependent; we recommend verifying Tm in your buffer and adjusting length or adding stabilizing sugars (2′-OMe/MOE/LNA) as needed.
Please avoid confidential details; we can arrange an NDA if needed.
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