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Epigenetic & Base Analog Oligonucleotide Modifications

Custom DNA/RNA oligos incorporating 5‑mC, 5‑hmC, 5‑fC, 5‑caC, 5‑hmU, m6dA/m6A, zebularine and more — for methylation mapping, TET/ADAR pathway studies, enzyme validation, controls, and calibration standards.

45+ Years ISO 9001 / 13485 RUO → GMP‑like Bench → Kilo
Overview Products & Codes Design Notes FAQ Speak

Overview

Bio‑Synthesis manufactures epigenetic and base‑analog–modified oligonucleotides to support methylation profiling, TET oxidation studies, reader/writer/eraser enzyme assays, antibody validation, and assay calibration. We provide DNA and RNA formats, single‑ or double‑stranded, and match purification/QC to your use case (HPLC/UPLC, LC‑MS, OD260; optional endotoxin/residuals/stability).

5‑mC 5‑hmC 5‑fC 5‑caC 5‑hmU m6dA m6A Zebularine 8‑oxo‑dG 2‑Aminopurine 7‑deaza A/G
  • Formats: ssDNA/dsDNA, ssRNA, siRNA; tube, vial, or plate packaging with labels/barcodes.
  • Scale: discovery µmol to gram/kilogram‑class supply with RUO→GMP‑like documentation.
  • Expert help: sequence design, placement strategies, and control sets for method development.

Products & Codes

Epigenetic & Base Analogs

Hide products and Notes
Cytosine family Uracil/Thymine family Adenine family Other analogs
Product Description Application Code
Cytosine Family (DNA/RNA)
5-Carboxy dC 5-carboxy-2′-deoxycytidine (5-caC) TET pathway; oxidation controls; antibody validation [5-ca-dC]
5-formyl dC 5-formyl-2′-deoxycytidine (5-fC) Intermediate in active demethylation; standard curves [5-for-dC]
5-hm dC (5-Hydroxymethyl-dC) 5-hydroxymethyl-2′-deoxycytidine (5-hmC) Oxidative demethylation studies; antibody calibration [5hmdC]
5-methyl deoxycytosine 5-methyl-2′-deoxycytidine (5-mC) Methylation controls; enzyme assays; qPCR standards [5mdC]
5-methyl-Cytosine (RNA) 5-methyl-cytidine (5-mrC) RNA methylation biology; reader assays [5mrC]
Uracil / Thymine Family
5-Hydroxymethyl-dU 5-hmU (deoxyuridine) Oxidative deamination models; antibody controls [5hmdU]
5-formyl-dU 5-fU (deoxyuridine) Damage/repair studies; calibration standards [5for-dU]
Adenine Family (DNA/RNA)
N6-Methyl dA (m6dA) N6-methyl-2′-deoxyadenosine Epigenetic adenine marks; enzyme validation [m6dA]
N6-Methyl rA (m6A) N6-methyl-adenosine RNA methylation (m6A) pathway; reader/writer assays [m6A]
Other Analogs & Probes
rZebularine Ribo-zebularine DNMT inhibitor analog; control studies [rZ]
Zebularine — deoxy-5-methyl Deoxy-zebularine (5-methyl) DNMT/repair pathway tools [dZ-5me]
8-oxo-dG 8-oxoguanine lesion analog DNA damage/repair; oxidative stress assays [8oxo-dG]
2-Aminopurine Fluorescent adenine analog Structure/function; monitoring base stacking [2AP]
7-deaza-A / 7-deaza-G Base analogs lacking N7 Structure probing; polymerase studies [7dz-A]/[7dz-G]
  • Placement: Introduce analogs at defined sites or as whole‑strand variants for controls/calibration.
  • Verification: We recommend LC‑MS identity and HPLC/UPLC purity for all epigenetic analog sets.

Design Notes

Practical Tips

  • Controls: Order matched pairs (e.g., 5‑mC / 5‑hmC / 5‑fC / 5‑caC) to build standard curves and antibody validation sets.
  • Strand context: Sequence context (CpG, CpH) can affect antibody and enzyme readouts; provide target locus if relevant.
  • RNA work: For m6A or 5‑mrC, consider 2′‑mods (2′‑OMe/2′‑F/MOE) when nuclease resistance is required.
  • QC & docs: Add optional endotoxin/residuals/stability when materials are used in vivo or for tech transfer.

Need a matched epigenetic standards set?

We’ll design a panel (5‑mC/5‑hmC/5‑fC/5‑caC, m6A/m6dA, etc.) with the right lengths, placements, and QC to fit your assay.

Speak to a Scientist Browse All Modifications

FAQ

Which formats do you support (DNA/RNA; single/duplex)?

ssDNA/dsDNA and ssRNA, plus siRNA duplexes on request. We’ll recommend placement (single site vs multi‑site) based on your assay.

Can you provide matched epigenetic control sets?

Yes—common kits include 5‑mC/5‑hmC/5‑fC/5‑caC ladders, m6A/m6dA pairs, and mixed panels for antibody or enzyme validation.

What QC do you recommend?

LC‑MS identity and HPLC/UPLC purity are standard. Optional tests include endotoxin, residual solvents/moisture, and time‑scheduled stability.

Can you help with assay design and packaging?

Absolutely—sequence design, control placement, formulation, and plate/vial packaging with labels/barcodes are all available.

Speak to a Scientist

Please avoid confidential details; we can arrange an NDA if needed.

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Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

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