Large Scale Peptide Synthesis

Scale peptides from multi-gram to kilogram quantities with a process-driven approach focused on batch consistency, impurity control, and fit-for-purpose QC. Our chemists support difficult sequences and program-aligned documentation for research through regulated pathways.

Multi-gram to kg scale
Process development
Difficult peptides
Custom purification
HPLC/MS QC

Overview

Large scale peptide synthesis is not simply increasing batch size. As peptide loads rise, issues such as incomplete coupling, aggregation, scale-dependent impurity growth, and purification recovery can become decisive. Bio-Synthesis applies process development to reduce scale risk and deliver reproducible, well-characterized material.

If your project involves hydrophobic sequences, long peptides, disulfide bonds, phosphorylation, or other complex features, we will recommend a practical synthesis, purification, and QC strategy aligned with your intended use.

Process-driven scale-up Impurity control Fit-for-purpose QC ISO 9001:2015 / ISO 13485:2016 45+ Years of Expertise U.S. Facilities – Texas
Scalable Process Design

Feasibility review, resin/loading strategy, coupling optimization, and impurity controls built for scale.

Purification Strategy

Scale-appropriate purification planning (including multi-step options) to balance purity and recovery.

QC & Documentation

COA and optional data packages; support for research through regulated pathway planning.

Specifications That Define Your Large-Scale Order

For the most accurate quoting and fastest start, include these details. If you’re unsure, share your application and we’ll recommend a practical specification.

Specification What to provide Why it matters at scale
Sequence 1-letter amino acid code; indicate non-standard residues Complex motifs may require alternative chemistries or staged synthesis
Scale (quantity) Target mass (e.g., 10 g, 100 g, 1 kg) Impacts resin choice, vessel sizing, purification, and schedule
Purity target Fit-for-purpose (e.g., crude/desalted, ≥70%, ≥85%, ≥95%) Higher purity may reduce yield and increase cost at scale
Salt / counter-ion TFA, acetate, or preferred form Can affect solubility, compatibility, and downstream methods
Modifications Termini, labels, phosphorylation, cyclization, PEG/lipid, linkers May change route design, purification complexity, and analytics
QC requirements COA needs + add-on testing (if required) Aligns release testing to your application and documentation needs

Need modifications? See Peptide Modifications. For complex sequences, see Difficult Peptide Synthesis.

Large Scale Capabilities

Manufacturing Scope
  • Scale: multi-gram → hundreds of grams → kilogram (project-dependent)
  • Types: linear, cyclic, long peptides; hydrophobic sequences
  • Complexity: disulfide-rich, multi-phosphorylated, aggregation-prone motifs
  • Formats: crude, desalted, or purified; lyophilized options

For regulated needs, see cGMP Peptide Synthesis.

Analytics & QC Options
  • HPLC/UPLC purity and impurity profiling
  • Mass spectrometry identity confirmation (MS/LC-MS as appropriate)
  • Counter-ion analysis (as requested)
  • Residual solvent testing (as requested)
  • Endotoxin / bioburden testing (when required)
  • Additional characterization and data packages upon request

Learn more: Peptide Analytics & QC.

Scale-Up Workflow

Large-scale projects typically move through feasibility review, optimization/pilot synthesis, full production, and release testing. This structure reduces late-stage failure risk and improves lot-to-lot consistency.

Scale-Up Peptide Synthesis Workflow from feasibility to batch delivery

Figure: Large-scale peptide synthesis workflow from feasibility to batch delivery

At-a-glance summary
  • Feasibility assessment: sequence and scale evaluation
  • Process development: optimize coupling and purification
  • Pilot manufacture: small-scale trial production
  • Batch delivery: full-scale QC & documentation

This workflow reduces late-stage risk and improves lot-to-lot consistency as you move to larger quantities.

1) Feasibility Review

Sequence risk assessment, resin/loading strategy, impurity risks, and purification planning.

2) Pilot & Optimization

Optimize coupling/deprotection and confirm purification performance before scaling.

3) Production & Release

Controlled manufacturing, purification, QC testing, and COA aligned to your requirements.

Tip: Over-specifying purity can reduce yield and increase cost at scale. If you share the application, we’ll recommend a practical target.

Applications

Preclinical & Translational Research

Expanded in vivo studies, method development, and reference material.

Diagnostics & Assay Manufacturing

Consistent peptide lots for assays, controls, and kit manufacturing.

Regulated Program Planning

Early alignment for future GMP, clinical, or commercial supply requirements.

FAQ

What is considered large scale peptide synthesis?

Large scale typically refers to production beyond milligram quantities—often tens of grams through kilogram levels depending on sequence complexity and specifications.

Can difficult or hydrophobic peptides be produced at scale?

Yes. Difficult sequences may require custom strategies and realistic purity/yield expectations. Provide sequence context so we can recommend an appropriate route.

Do you support GMP manufacturing?

GMP-aligned workflows and documentation can be supported depending on program requirements. Share your quality expectations so we can propose the right pathway.

What analytical testing can be included in the COA?

Common items include HPLC/UPLC purity, mass confirmation, and optional tests such as counter-ion analysis, residual solvents, endotoxin, or bioburden when required.

What information do you need for a quote?

Sequence (with modifications), target quantity, purity/specs, salt/form, QC requirements, and intended use.

How long does large-scale synthesis take?

Timelines vary by complexity, scale, purification needs, and QC. A feasibility review enables accurate scheduling upfront.

Should I request ≥95% purity for all large-scale orders?

Not always. Higher purity can reduce yield and increase cost at scale. The best target depends on application sensitivity and whether impurities will impact interpretation.

Do you offer catalog peptides for quick delivery?

Yes. If you need smaller quantities quickly, browse Catalog Peptides.

CONTACT

Request a Quote for Large-Scale Peptides

Share your sequence, target scale, purity target, modifications, and QC/documentation needs. Our team will recommend a practical synthesis and QC strategy and provide a quote.

Helpful links: Difficult PeptidesAnalytics & QCcGMP Peptides

Tip: If the peptide is hydrophobic/long/cysteine-rich, include that context so we can route the request to the right synthesis strategy.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.