Design Tips
- Start simple: Evaluate
Doubler/Trebler at a terminus; add internal
branching after Tm verification.
- Balance hydrophilicity: Prefer
TEG/PEG branches for hydrophobic payloads.
- Avoid critical motifs: Keep
branch points out of seed regions (siRNA) and
RNase-H cores (gapmers).
- Valency vs length: More arms
may require longer linkers to avoid
self-quenching/aggregation.
- Spacer choice: Use TEG/PEG
branches (C6–PEG12) to offset steric bulk and
preserve hybridization Tm.
- Placement: Start at termini;
for internal branchers (e.g., 5-Me-dC), avoid
seed regions in siRNA and core DNA gaps in
RNase-H designs.
- Valency: Trebler (3-arm) and
dendrons (G1–G4+) scale signal/payload but may
raise viscosity and retention; plan
purification.
- Purification/QC: HPLC plus SEC
or diafiltration recommended for dendrimer
conjugates; MS may show broadened
envelopes—report average loading.
- Applications: Aptamer
multimerization, probe signal amplification,
nanoparticle assembly, high-density surface
immobilization.
Key Modification Strategies
- Dendrimer Conjugation (e.g.,
PAMAM dendrimer, bis-MPA dendrons)
- Branched Linkers at the 5′, 3′,
or internal positions
- Multivalent Ligand Display
(e.g., GalNAc, peptides, dyes)
- Spacer-enhanced branching
(e.g., PEG-based, alkyl, or nucleotide spacers)
Two main strategies exist for
synthesizing oligonucleotide dendrimers. In the
divergent approach, the structure
grows outward from the core to the periphery.
In the convergent approach, growth
proceeds inward, from the periphery toward the
center. The number of branches added at each step
dictates how many cycles are required and ultimately
controls both the size of the dendrimer and the
density of groups displayed on its surface. The
chemical nature of these terminal groups defines the
overall properties of the molecule.
Our dendrimeric modifiers allow dendrimer structures
to be built either on top of a conventional
monomeric oligonucleotide or directly on the solid
support at the 3′ end.
Furthermore, monomeric and dendrimeric sequence
segments can be synthesized in different lengths and
orientations by applying either 3′ or 5′
oligonucleotide synthesis chemistries.
Symmetric Doubler 2DNA
The symmetric doubler produces branches with the same
oligo sequence.
Symmetric Trebler 3DNA
The symmetric doubler produces branches with the same
oligo sequence
Different generations of dendrimeric oligonucleotides