Site-defined post-translationally modified peptides for signaling, epigenetics, binding assays, and LC–MS workflows—phospho, glyco, acetyl, methyl, lipid, UBLs, and more, with fit-for-purpose QC.
Site-defined, homogeneous PTM peptides—panel-ready for binding maps, enzyme on/off models, and assay controls.
Post-translational modifications (PTMs) are biologically encoded covalent changes introduced after translation (e.g., phosphorylation, glycosylation, acetylation). PTMs regulate activity, localization, stability, and molecular recognition by altering charge, conformation, and interaction surfaces.
Bio-Synthesis specializes in synthetic, site-defined, homogeneous PTM peptides, including single-site and multi-PTM peptide panels with controlled stoichiometry for mechanistic, quantitative, and assay-critical studies.
Bio-Synthesis produces site-defined, homogeneous PTM peptides (single-site or multi-PTM) with purification and fit-for-purpose analytical verification (HPLC/LC–MS) delivered with a COA—built for signaling, epigenetics, binding assays, and quantitative LC–MS workflows.
Tip: For most studies, request a set: unmodified + PTM + site variants to confirm positional specificity.
We synthesize site-defined, post-translationally modified peptides across major PTM classes. Choose a category below or request a custom multi-PTM panel.
Ser/Thr/Tyr phosphorylation peptides model defined signaling states for kinase/phosphatase assays, phospho-dependent binding, and MS workflows. Order matched unmodified controls and single-site variants to confirm positional specificity.
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Site-defined N-linked (Asn) and O-linked (Ser/Thr) glycopeptides support vaccine epitopes, receptor binding, and antibody validation while avoiding glycan microheterogeneity. Specify the glycan identity (e.g., GalNAc or tri-GalNAc) and linkage type.
N-terminal acetylation and ε-N-acetyllysine peptides are core tools for epigenetics. Provide the exact site(s) and consider combinatorial panels with methylation or phosphorylation for PTM crosstalk studies.
Lys/Arg methylation is state-specific (me1/me2/me3; symmetric vs asymmetric dimethyl-Arg). Define the state explicitly and use panels to map reader-domain selectivity.
Ubiquitination and UBL models (Ub/SUMO/NEDD8) enable proteostasis and enzyme mechanism studies. Provide the target lysine position and desired construct format/linkage for your E3/DUB workflow.
Myristoylation/palmitoylation/prenylation increase hydrophobicity and membrane association. For assay-friendly handling, discuss solubility-oriented formats (spacers, composition tuning) and aliquoting.
Tyrosine O-sulfation is common in extracellular recognition (e.g., chemokine receptors). Sulfated peptides are useful for binding assays and structure–function mapping.
Mono-ADP-ribosylation (often Ser) is central to DNA repair signaling. Define the acceptor residue and position; plan handling conditions that preserve PTM integrity.
We also support citrullination, hydroxylation, oxidation/redox states, disulfide architectures, pyroglutamate formation, C-terminal amidation, and maturation/truncation series. Ask for a multi-PTM panel if you’re mapping crosstalk.
Quick rule: If the modification exists naturally in vivo and controls signaling or regulation → choose a PTM peptide. If the modification is added for detection, capture, or assembly → choose a chemically conjugated peptide.
Post-translational modifications (PTMs) are covalent changes found in vivo and installed by enzymes. If your goal is to model native signaling, regulation, or recognition, use PTM terminology and order site-defined PTM peptides.
Best-fit: mechanistic studies, PTM-dependent binding, epigenetics, pathway switching, and biologically faithful controls.
Chemical conjugations attach functional handles that help you detect, capture, immobilize, or deliver peptides. They’re valuable, but they are not biological PTMs. Label these accurately to avoid confusing “modified peptide” searches.
Best-fit: detection & immobilization, multiplex assays, conjugate assembly, delivery systems, and workflow acceleration.
Bio-Synthesis tip: If you’re unsure, tell us your assay type (binding vs MS vs cell-based) and whether the modification mimics an in vivo state or serves as a workflow handle—we’ll recommend the correct format.
Use this matrix to pick the right PTM format for your experiment. We can provide matched unmodified controls and site variants.
Design tip: For functional studies, order PTM + unmodified + site variants together to avoid ambiguous results and shorten iteration cycles.
Handling note: If your workflow involves harsh conditions or long incubations, share details early so we can recommend formats that preserve PTM integrity.
Yes. PTM peptides used in research are typically chemically synthesized to install post‑translational modifications at defined residue positions and stoichiometry. Synthetic PTM peptides avoid the heterogeneity of enzymatic or recombinant expression systems and are preferred for mechanistic studies, quantitative LC–MS workflows, and assay controls.
A PTM peptide is a synthetic peptide containing a biologically relevant post-translational modification (e.g., phosphorylation, glycosylation, acetylation) installed at a defined site and stoichiometry.
Chemical conjugations (dyes, biotin, PEG) are assay handles. PTMs are biological regulatory modifications used to mimic native signaling states.
Yes. We support site-defined multi-PTM peptides for crosstalk studies. Please specify exact PTM sites and states.
Provide sequence (N→C), PTM site(s) and state(s), terminal format, purity/quantity, and intended application.
Some PTMs are labile. We provide handling guidance and stability-oriented formats when needed.
Send your sequence(s), PTM site(s), and specifications. We’ll respond with a synthesis plan and QC options aligned to your assay or MS workflow.
What happens next: Our technical team typically reviews requests within 1 business day and responds with feasibility notes, recommended chemistry/linker options, a QC plan, and pricing.
If you’re not sure which coupling is most robust, share the payload functional groups-our team will recommend a handle + linker plan to minimize heterogeneity and confirm identity by fit-for-purpose analytics.
Peer-reviewed background on post-translational modifications and PTM peptide applications.
We can tailor a reading list to your PTM (phospho, glyco, acetyl, methyl, UBL) and assay type on request.
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