Stable‑isotope labeling for LC‑MS quantitation, kinetic isotope effects, and NMR tracing. Example product: 8‑D‑dmf‑dG phosphoramidite for site‑specific incorporation of C8‑deuterated guanine under standard DNA synthesis workflows.
Deuterated base oligonucleotides incorporate one or more heavy‑isotope (²H/D) substitutions at defined positions (e.g., C8 of purines). At a chosen site, these isotopes provide a mass offset for precise LC‑MS quantitation, enable kinetic isotope effect (KIE) measurements, and support NMR/IR tracing, all while preserving canonical base‑pairing.
Common sites: C8 purines (G/A), 5/6 pyrimidines (U/T/C). Choose a site that balances synthetic stability and analytical resolution.
Amidites such as 8‑D‑dmf‑dG run under standard phosphoramidite chemistry (0.1 M ACN). Use typical detritylation/coupling times.
Use time‑limited base (NH4OH or AMA). Avoid prolonged high‑temperature deprotection to minimize H/D exchange risk.
Isotope‑dilution workflows with D‑labeled oligos provide accurate, matrix‑tolerant quantitation.
Measure KIEs in polymerase, glycosylase, or nuclease reactions to interrogate transition states and rate‑limiting steps.
Deuterium labeling aids assignment and signal tracking in complex systems.
Send sequence, labeled position(s), assay (MS/KIE/NMR), and purification level. We’ll return a tuned design and QC plan.
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We can align specific literature protocols to your design upon request.
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