Rapid. Reliable. Secure
Hydrolysis (TaqMan®‑style) • MGB • LNA‑enhanced • Multiplex
qPCR probes deliver real‑time, sequence‑specific fluorescence for accurate quantitation. Compared to intercalating dyes, probe‑based assays provide higher specificity, lower background, and robust multiplexing. Bio‑Synthesis designs, manufactures, and QC‑tests custom hydrolysis, MGB, and LNA‑enhanced probes from RUO to GMP.
Dual‑labeled probes carry a 5′ reporter and a 3′ quencher. During extension, the polymerase’s 5′→3′ exonuclease activity cleaves the probe, physically separating dye and quencher. The resulting fluorescence is proportional to the accumulating amplicon, enabling precise Cq determination.
Standard dual‑labeled probes for most assays; broad dye/quencher compatibility and strong performance across cyclers.
MGB raises Tm to enable shorter probes; LNA increases affinity/specificity in AT‑rich or structured regions and for SNP discrimination.
Share your instrument model and filter sets—we’ll propose dye combinations and concentrations for clean, balanced multiplex runs.
Hydrolysis probes generate fluorescence only when the correct target is amplified, improving specificity and multiplex compatibility compared to intercalating dyes that report total dsDNA.
Use MGB for shorter probes with high Tm or SNP discrimination; use LNA to boost affinity/specificity at difficult positions or in AT‑rich regions.
Yes. Provide instrument filters and target list; we’ll propose dye sets, concentrations, and plate maps.
Tell us your sequence, modification, linker requirments - our team will send a tailored plan and quote.
By submitting, you agree to be contacted regarding your request.
Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.