CuAAC and copper-free SPAAC click-ready peptides with site-defined handle placement, spacer/linker tuning, purification, QC & COA.
Related: Peptide Modifications, Cleavable Peptide Linkers, Isotope-Labeled Peptides. If your sequence is challenging, see difficult peptide synthesis.
Click chemistry peptides are peptides synthesized with a defined reactive handle—most commonly azide, alkyne, DBCO, or BCN—to enable rapid, selective conjugation to payloads such as fluorophores, drugs, PEG, lipids, oligonucleotides, proteins, or surfaces. This modular approach supports reproducible conjugates with predictable stoichiometry and analytics.
The biggest differentiator is not “whether a handle exists,” but whether the handle is placed where it stays accessible and does not disrupt binding/uptake. Bio-Synthesis specializes in site-defined click handle placement with optional spacers to control sterics and solubility.
Most successful conjugates define: (1) click reaction, (2) handle placement, (3) payload constraints, (4) analytics requirements.
Send your sequence and downstream context (in vitro vs live-cell/in vivo, payload class, and preferred handle). We’ll recommend CuAAC vs copper-free SPAAC, define handle placement, and align synthesis/QC to your conjugation workflow.
Azide peptide + terminal alkyne payload → stable triazole linkage (high conversion, robust conditions).
In vitro conjugation, maximum yield, robust scale-up, stable analytics.
Copper may be incompatible with live cells, in vivo work, or copper-sensitive payloads.
Azide peptide + strained alkyne (DBCO or BCN) → triazole linkage without metal catalyst.
Live-cell / in vivo labeling, copper-sensitive payloads, metal-free workflows.
DBCO vs BCN choice, spacer length, and handle placement (sterics/kinetics/solubility).
Click chemistry is preferred when you need selectivity, reproducible stoichiometry, and clean analytical readouts.
We place azide/alkyne/DBCO/BCN where the handle stays exposed in solution, with spacer options to avoid steric shielding and activity loss—common failure modes on generic click peptide pages.
Instead of listing reactions, we recommend the correct chemistry based on copper tolerance, payload sensitivity, and biological context—especially for copper-free SPAAC (DBCO/BCN) workflows.
Our deliverables and characterization are aligned to your conjugation plan (identity, purity, and handle confirmation), helping you avoid rework after purchase.
Note: Many vendors (including Creative Peptides and similar providers) can supply click handles. The differentiator is whether handle placement and spacer/linker choices are engineered to protect function and conjugation efficiency.
Not sure which handle to choose? Send your payload type and biological context; we’ll recommend CuAAC vs SPAAC and the best handle orientation.
Design focus: controlled stoichiometry, linker/spacer selection, and reproducible analytics.
Design focus: bioorthogonal chemistry and minimal impact on binding/uptake.
Design focus: geometry, spacing, and stability of the triazole connection.
If your application is unclear, tell us where the conjugation occurs (bench vs biological system). We’ll map the click chemistry accordingly.
Tip: if you must place a bulky handle (e.g., DBCO) near an epitope, spacer tuning often reduces steric interference.
This reduces iteration cycles and improves first-pass success.
For the fastest quote, send the items below. We’ll respond with feasibility notes, a recommended click strategy, QC options, and pricing.
Project-dependent add-ons can be aligned to your regulatory and analytical context.
Choose CuAAC for robust in vitro conjugation and maximum conversion. Choose SPAAC (azide + DBCO/BCN) when copper must be avoided (live-cell/in vivo or copper-sensitive payloads).
We provide azide-, alkyne-, DBCO-, and BCN-modified peptides, with N-terminal, C-terminal, or site-defined side-chain placement (e.g., Lys/Cys) and optional spacers.
Yes. Provide your preferred position or functional constraints; we can propose site-defined placements that preserve function and maintain handle accessibility.
Yes. Intramolecular CuAAC or SPAAC can generate stable cyclic constructs when geometry and spacing are designed appropriately.
Most issues are architectural: the handle is buried, sterically shielded, or aggregation limits accessibility. Spacer tuning and handle placement usually resolve this.
Typical deliverables include analytical HPLC purity profiling, MS identity confirmation, and a COA. Additional QC can be aligned to your workflow.
For the fastest quote, send your sequence, desired click handle (azide/alkyne/DBCO/BCN), placement preference, payload context, quantity/purity, and any documentation requirements.
What happens next: Our technical team reviews your request and replies with a recommended click handle strategy, placement plan, and synthesis/QC approach aligned to your conjugation workflow.
Stable citations for click chemistry fundamentals and bioorthogonal conjugation used in peptide science.
Related: Peptide Modifications, Cleavable Peptide Linkers, Isotope-Labeled Peptides, Ready-made catalog peptides.
Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.