High-throughput, sequence-defined peptide libraries built to your design — ideal for epitope mapping, lead discovery, SAR, and immunology screening. Choose library format, peptide length/offset, purity, and delivery in plate-ready layouts with robust QC.
Our custom peptide library synthesis service is part of our peptide synthesis services and produces collections of peptides designed for systematic screening. We support libraries for linear epitope mapping (overlapping peptides), residue importance studies (alanine or positional scanning), focused motif exploration, and custom experimental designs.
Ideal for epitope mapping, screening, and SAR. Send your target sequence(s) and assay context — we can recommend peptide length, offset, controls, and deliverable format.
Typical ranges shown below. Exact specs depend on library size, sequence complexity, and required deliverables.
Systematically cover an entire protein or region using partially overlapping peptides for high-resolution mapping.
Identify critical residues by substituting alanine (or another residue) at each position across a lead sequence.
Probe sequence space by fixing positions and varying others with defined amino acid sets.
Explore variations around a known motif or binding region with rational constraints.
Generate broader diversity for screening programs with controlled complexity and deliverable format.
Receive pools grouped by region, function, or assay design, with optional pre-/post-pooling verification.
Build overlapping libraries, scanning libraries, and other common formats using our online design tool, then send the output to us for synthesis.
Share a target sequence (or a list of peptide sequences) and choose the library format. We synthesize, verify, and deliver your library in plate/tube layouts with the documentation you need to run assays fast.
For workflows that require consistent, assay-ready reference peptides, we can produce libraries as individual peptides (plate/tube arrays) with optional stable‑isotope labeling, PTM panels, and documentation that supports streamlined method setup and data review.
Common heavy labels are available on request (single peptides or full libraries).
Tell us your preferred enrichment level and reporting needs.
Select the QC depth that fits your workflow and budget.
Linear epitope discovery for antibodies, B-cells, and T-cells using overlapping peptide sets.
Identify binders and bioactive sequences with focused or combinatorial library strategies.
Alanine scanning, truncation, and rational substitutions to pinpoint key residues and improve performance.
Substrate libraries and modified peptides for signaling and enzymology workflows.
Map immunogenic regions and test candidate sequences efficiently in plate-ready formats.
Peptide panels and libraries for method development and targeted assays (project-dependent).
We also have a wide selection of ready-made, on-demand catalog peptides.
If your library requires specialized labels, linkers, or conjugation strategies, ask about our bioconjugation and custom modification support.
Send sequences (or target protein/regions), desired library format (overlap/offset or scanning plan), quantity per peptide, purity requirements, delivery format (plate/tubes), and any modifications/QC add-ons.
Yes — use our online peptide library designing tool to generate common library formats and export sequences for synthesis.https://new.biosyn.com/peptidescreeningtools.aspx
Yes. We can deliver libraries in plate-ready layouts or tube arrays and provide a map (sequence list + positions) to streamline screening.
Typical QC includes MS identity confirmation with COA. Optional HPLC purity analysis and custom data packages are available.
Yes — we can prepare custom pools according to your specification, with optional verification strategies depending on project needs.
Yes. We can incorporate common 13C/15N labels (e.g., Lys/Arg) on individual peptides or across an entire library. Share your sequences, preferred labeling strategy, and any reporting requirements.
Yes. We can align sequences to your method needs (for example, preferred C‑terminal residues), deliver in 96/384 layouts or tube arrays, and provide plate maps. Optional PTM panels and data packages are available.
Libraries are typically supplied lyophilized. Store sealed containers as recommended in the handling guide/COA and minimize moisture exposure after opening.
Share your target protein/regions or sequence list, preferred library format (overlap/offset or scanning plan), quantity per peptide, purity, plate/tube delivery, and any modifications or QC add-ons. We’ll recommend a practical specification and provide a quote.
Tip: For large libraries, attach a spreadsheet (one sequence per line) and include any required controls/replicates.
Need help designing the library? Use our online tool: Peptide Library Design Tool.
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