Precision Labeling & Functional Handles

Isotope-labeled peptides, cleavable linkers, and click-ready handles engineered for reproducible analytics and downstream chemistry.

Isotope-labeled Cleavable linkers Azide / Alkyne SPAAC / CuAAC Quant standards

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Overview

Bio-Synthesis provides precision labeling and functional handle peptide synthesis services for workflows that depend on accurate mass, predictable reactivity, and reproducible conjugation. Common applications include quantitative LC–MS standards, MS/MS validation peptides, targeted labeling (dyes/biotin/drugs), and modular assembly using click-ready chemistries.

Why labels & handles matter
  • Quantitation: isotope-labeled internal standards improve accuracy.
  • Control: defined handles enable site-specific conjugation.
  • Compatibility: linker/handle selection reduces side reactions.
Typical applications
  • Stable isotope dilution (SID) LC–MS quantitation
  • Proteomics standards and method validation
  • Click-enabled bioconjugation and immobilization
  • Cleavable linkers for controlled release or traceless tags

Best-fit when you need

Defined label pattern

Uniform or residue-specific isotope placement.

Orthogonal chemistry

Azide/alkyne workflows with minimal cross-reactivity.

Release control

Cleavable linkers tuned to your trigger and stability needs.

Quick Specification Table (Services at a Glance)

Use this table to map your project to the right labeling or handle strategy. For details, see the service sections below.

Service What it is Key design inputs Common use cases
Isotope-Labeled Peptides Peptides containing stable isotopes (e.g., 13C, 15N, 2H) for analytical workflows. Isotope(s), labeling pattern (uniform vs residue-specific), positions, purity/scale. Internal standards, quantitation, proteomics validation.
Cleavable Linker Peptides Peptides incorporating cleavable linkers for controlled release or traceless removal. Trigger (enzyme/pH/reduction/photo), stability requirements, cleavage site and linker length. Controlled release, traceless tags, assay workflows.
Click Chemistry Peptides Peptides with azide/alkyne (or related) handles for modular conjugation. Handle type (azide/alkyne), CuAAC vs SPAAC preference, attachment site, downstream partner. Bioconjugation, immobilization, labeling and imaging.

Related service pages (internal links)

For full options, pricing drivers, and examples, visit the dedicated pages:

Technical Notes — Information that speeds quoting
Project inputs
  • Sequence(s) and desired service (isotope / cleavable linker / click handle).
  • Target purity/scale and intended use (quant standards vs conjugation vs assays).
  • For click: conjugation partner and CuAAC vs SPAAC preference.
Success criteria
  • Define the readout (quantitation accuracy, conjugation efficiency, release performance).
  • Specify stability constraints (storage, buffers, reducing agents, light sensitivity).
  • Tell us if you need extra QC (label pattern confirmation, cleavage testing).

Isotope-Labeled Peptides Services

Isotope-labeled peptide synthesis supports stable isotope dilution and method validation by incorporating defined isotopes and labeling patterns. Specify whether you need uniform labeling (entire peptide) or residue-specific labeling at defined positions.

Design option Typical choices
Isotope type 13C, 15N, 2H (project-dependent).
Label pattern Uniform vs residue-specific vs terminal labeling.
Quant use Internal standards, calibration curves, MS/MS validation peptides.
Documentation Provide target purity/scale and reporting needs for your workflow.
Technical Notes — Label placement & performance
Design guidance
  • Match the labeled peptide to your native analyte (sequence and modifications).
  • Tell us your MS method so we can advise label placement if needed.
  • Define whether deuterium is acceptable for your application.
QC options
  • LC–MS confirmation and purity reporting (typical).
  • Optional confirmation of labeling pattern upon request.
  • Batch reproducibility support for quantitative workflows.

Related: Isotope-labeled peptides service page.

Cleavable Linker Peptides Services

Cleavable linker peptides incorporate linkers that break under defined conditions to release tags, payloads, or fragments. Specify the trigger and stability requirements so the linker performs reliably in your workflow.

Design option Typical choices
Cleavage trigger Enzymatic, pH-sensitive, reduction-sensitive, photo-cleavable (project-dependent).
Stability window Stable during storage/handling; cleaves under defined trigger conditions.
Placement Between peptide and tag/payload; or internal for controlled fragmentation.
Linker length Tuned to accessibility and steric needs.
Technical Notes — Stability vs cleavage
Design guidance
  • Define trigger conditions (pH, reductant, enzyme, light) and exposure time.
  • Tell us the payload/tag chemistry to ensure compatibility.
  • Consider spacer length if cleavage site accessibility is limiting.
QC options
  • LC–MS confirmation and purity reporting (typical).
  • Optional stability/cleavage checks under your specified conditions.
  • Documentation aligned to your application.

Related: Cleavable linker peptides service page.

Click Chemistry Peptides Services

Click chemistry peptide synthesis installs azide/alkyne (or related) handles for modular conjugation. Tell us whether your downstream workflow uses copper-catalyzed click (CuAAC) or strain-promoted click (SPAAC) to guide handle selection and compatibility.

Design option Typical choices
Handle type Azide or alkyne; strain-promoted options for copper-free workflows.
Workflow CuAAC vs SPAAC; specify copper sensitivity if applicable.
Attachment site N-terminus, C-terminus, or side chain handle chosen to preserve activity.
Partner molecule Dye, biotin, oligo, small molecule, surface, or protein partner.
Technical Notes — Conjugation efficiency
Design guidance
  • Define the click partner and whether you require copper-free conditions.
  • Use spacers/linkers to reduce steric hindrance for bulky partners.
  • Tell us if additional handles (cysteine/biotin) are needed in the same peptide.
Deliverables
  • Purified click-ready peptide + CoA (typical).
  • LC–MS confirmation; optional additional analytics as needed.
  • Guidance on storage and compatibility with common click reagents.

Related: Click chemistry peptides service page.

FAQ

What information do you need for isotope-labeled peptide synthesis?

Provide the sequence, isotope(s) required, labeling pattern (uniform vs residue-specific), target purity/scale, and your analytical method (LC–MS, MS/MS, NMR).

When should I choose a cleavable linker peptide?

Choose cleavable linkers when you need controlled release or traceless tag removal. Specify the trigger and required stability during storage and handling.

Do you support copper-free click chemistry peptides?

Yes. If copper is incompatible with your system, we can plan copper-free click strategies. Tell us your partner molecule and workflow constraints.

Can I combine isotope labels with click handles?

Yes. Provide the order of operations and any constraints; we can plan label placement and handle selection for compatibility.

Can I combine isotope labels with click handles?

Yes. Provide the order of operations and any constraints; we can plan label placement and handle selection for compatibility.

Do these peptides require extra QC?

Often. In addition to purity and LC–MS, projects may benefit from confirming labeling patterns, handle integrity, and linker stability/cleavage performance.

How should I specify formulation for reactive handles?

Tell us your storage conditions and buffer constraints. Some handles are sensitive to reducing agents, light, or specific pH ranges, and formulation can be adjusted accordingly.

CONTACT

Speak to a Labeling & Functional Handle Scientist

Share your peptide sequence(s), labeling/handle type (isotope, cleavable linker, click chemistry), target purity/scale, and intended application. We’ll recommend practical specifications and a synthesis/QC plan aligned to your goals.

Tip: If the peptide is hydrophobic/long/cysteine-rich, include that context so we can route the request to the right synthesis strategy.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.