Steric-blocking antisense conjugates with defined peptide attachment, purification, and fit‑for‑purpose QC documentation.
High-quality peptide–PMO and peptide–ThioMorpholino™ (TMO) conjugates for steric-blocking antisense delivery
Peptide–morpholino conjugates are chemically defined antisense constructs in which a peptide is covalently linked to a morpholino oligomer to support cellular association and delivery while preserving steric-blocking activity.
Bio-Synthesis provides peptide–morpholino conjugation services for programs using charge-neutral steric-blocking antisense agents, supporting both traditional PMO (phosphorodiamidate morpholino oligomers) and ThioMorpholino™ (TMO) platforms. We synthesize and qualify peptide–PMO and peptide–TMO conjugates using site-defined handles, practical conjugation routes (solid-phase or solution-phase), and analytical verification aligned to your program stage, with purification and fit-for-purpose QC documentation. This service complements our broader oligonucleotide conjugation capabilities.
Morpholino antisense agents typically act by blocking RNA interactions (for example, splice-site recognition or ribosome access), rather than recruiting enzymatic RNA-cleavage pathways. Peptide conjugation can support delivery concepts, including targeting ligands and CPP designs, while maintaining a single, chemically defined construct suitable for reproducible characterization and comparative studies.
This service complements our broader oligonucleotide conjugation and antisense modification capabilities.
Related services: Peptide Modifications, Peptide Bioconjugation, Cell-Penetrating Peptides (CPPs).
Morpholino antisense agents are commonly used as steric blockers to modulate splicing or translation by hybridizing to a complementary RNA region and blocking a biological interaction.
Peptide conjugation is used to support cellular association and delivery concepts, including receptor-targeting ligands and CPP uptake designs. Conjugates are designed to remain chemically defined for purification and analytical qualification.
Peptides in morpholino conjugates may support uptake via multiple pathways (e.g., receptor-mediated endocytosis for targeted ligands and other routes for cationic CPP designs). Peptide/linker selection balances solubility, stability, and analytical clarity with the intended delivery concept.
We support both traditional PMO and TMO constructs. Route selection (solid-phase vs solution-phase) depends on platform, handle placement, and purification/QC strategy.
Compared to PMO, ThioMorpholino™ (TMO) designs expand chemical flexibility by enabling phosphoramidite-style synthesis and broader modification strategies, as reported in the literature.
Figure: PMO vs TMO backbone linkage comparison and chimera concept. (Place your provided image in the same folder and keep the filename.)
These points summarize published findings; performance depends on sequence, chemistry map, target accessibility, and biological model.
Reference key: [1] PNAS 2022; [2] Int J Mol Sci 2024; [3] PubMed 2021.
Chemoselective coupling using defined amine/thiol handles (project-dependent).
Azide/alkyne-type handles for bioorthogonal coupling (program-dependent).
Selected based on whether release is required (program-dependent).
Peptide–morpholino conjugates can be assembled by solid-phase or solution-phase coupling. Route selection depends on platform, handle placement, and purification strategy.
QC and documentation depth can be adjusted to match discovery, preclinical, or later development needs.
PMOs are charge-neutral morpholino antisense analogs commonly used as steric blockers to modulate splicing or translation by RNA binding.
TMO uses a thiophosphoramidate internucleotide linkage. Literature reports compatibility with phosphoramidite-style solid-phase synthesis and broader modification/chimera options (design-dependent).
Yes. We can conjugate CPPs or targeting peptides using site-defined handles and practical coupling routes selected for construct stability and analytical clarity.
Platform (PMO/TMO), sequence and target region, desired peptide and attachment constraints, linker preference (stable/cleavable), scale, and purity/usage requirements.
For the fastest quote, send platform (PMO/TMO), sequence(s), target region, peptide (or “recommend”), desired attachment site constraints, linker preference (stable vs cleavable), quantity/purity targets, and intended use. We’ll recommend a practical route plus purification/QC aligned to your program stage.
P26-01-30
Peer-reviewed references supporting ThioMorpholino™ (TMO) chemistry and reported applications.
References provided for scientific context; Bio-Synthesis does not claim ownership of the cited works.
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