Steric-blocking antisense conjugates with defined peptide attachment, purification, and fit‑for‑purpose QC documentation.
High-quality peptide–PMO and peptide–ThioMorpholino™ (TMO) conjugates for steric-blocking antisense delivery
Peptide–morpholino conjugates are chemically defined antisense constructs in which a peptide is covalently linked to a morpholino oligomer to support cellular association and delivery while preserving steric-blocking activity.
Bio-Synthesis provides peptide–morpholino conjugation services for programs using charge-neutral steric-blocking antisense agents, supporting both traditional PMO (phosphorodiamidate morpholino oligomers) and ThioMorpholino™ (TMO) platforms. We synthesize and qualify peptide–PMO and peptide–TMO conjugates using site-defined handles, practical conjugation routes (solid-phase or solution-phase), and analytical verification aligned to your program stage, with purification and fit-for-purpose QC documentation. This service complements our broader oligonucleotide conjugation capabilities.
Morpholino antisense agents typically act by blocking RNA interactions (for example, splice-site recognition or ribosome access), rather than recruiting enzymatic RNA-cleavage pathways. Peptide conjugation can support delivery concepts, including targeting ligands and CPP designs, while maintaining a single, chemically defined construct suitable for reproducible characterization and comparative studies.
This service complements our broader oligonucleotide conjugation and antisense modification capabilities.
Morpholino antisense agents are commonly used as steric blockers to modulate splicing or translation by hybridizing to a complementary RNA region and blocking a biological interaction.
Peptide conjugation is used to support cellular association and delivery concepts, including receptor-targeting ligands and CPP uptake designs. Conjugates are designed to remain chemically defined for purification and analytical qualification.
Peptides in morpholino conjugates may support uptake via multiple pathways (e.g., receptor-mediated endocytosis for targeted ligands and other routes for cationic CPP designs). Peptide/linker selection balances solubility, stability, and analytical clarity with the intended delivery concept.
We support both traditional PMO and TMO constructs. Route selection (solid-phase vs solution-phase) depends on platform, handle placement, and purification/QC strategy.
Compared to PMO, ThioMorpholino™ (TMO) designs expand chemical flexibility by enabling phosphoramidite-style synthesis and broader modification strategies, as reported in the literature.
Figure: PMO vs TMO backbone linkage comparison and chimera concept. (Place your provided image in the same folder and keep the filename.)
These points summarize published findings; performance depends on sequence, chemistry map, target accessibility, and biological model.
Reference key: [1] PNAS 2022; [2] Int J Mol Sci 2024; [3] PubMed 2021.
Chemoselective coupling using defined amine/thiol handles (project-dependent).
Azide/alkyne-type handles for bioorthogonal coupling (program-dependent).
Selected based on whether release is required (program-dependent).
Peptide–morpholino conjugates can be assembled by solid-phase or solution-phase coupling. Route selection depends on platform, handle placement, and purification strategy.
QC and documentation depth can be adjusted to match discovery, preclinical, or later development needs.
PMOs are charge-neutral morpholino antisense analogs commonly used as steric blockers to modulate splicing or translation by RNA binding.
TMO uses a thiophosphoramidate internucleotide linkage. Literature reports compatibility with phosphoramidite-style solid-phase synthesis and broader modification/chimera options (design-dependent).
Yes. We can conjugate CPPs or targeting peptides using site-defined handles and practical coupling routes selected for construct stability and analytical clarity.
Platform (PMO/TMO), sequence and target region, desired peptide and attachment constraints, linker preference (stable/cleavable), scale, and purity/usage requirements.
For the fastest quote, send platform (PMO/TMO), sequence(s), target region, peptide (or “recommend”), desired attachment site constraints, linker preference (stable vs cleavable), quantity/purity targets, and intended use. We’ll recommend a practical route plus purification/QC aligned to your program stage.
P26-01-30
Peer-reviewed references supporting ThioMorpholino™ (TMO) chemistry and reported applications.
References provided for scientific context; Bio-Synthesis does not claim ownership of the cited works.
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