Custom RNA Synthesis

RNA Pricing — Unmodified, 2′-Fluoro, 2′-O-Methyl

ssRNA for antisense, ribozymes, aptamers, capture probes, and assay controls. Delivered RNase-free with deprotection/desalting; higher-grade purification on request.

RNA, 2′-Fluoro, 2′-O-Methyl RNA unmodified Oligonucleotides (price per base, USD)*
Product	Length	250 nmol scale	1 μmol scale	2 μmol scale	5 μmol scale	10 μmol scale	15 μmol scale
RNA Oligonucleotides	5–60 bases	$8.50/base	$18.00/base	$28.00/base	$55.00/base	$105.00/base	$155.00/base
2′-Fluoro RNA Oligo	5–150 bases	$8.50/base	$18.00/base	$28.00/base	$105.00/base	$109.00/base	$155.00/base
2′-O-Methyl RNA Oligo	5–150 bases	$8.00/base	$18.00/base	$28.00/base	$55.00/base	$105.00/base	$155.00/base
Phosphorothioate Linkages	—	$5.00/bond	$5.00/bond	$10.00/bond	$25.00/bond	$50.00/bond	$65.00/bond
Chimeric Linkages	—	$50.00	$75.00	$110.00	$225.00	$425.00	$610.00
* Minimum charge for 15-mer or 15 phosphorothioate linkages applies.
* For sequence over >150 bases, please contact us or request a quote.

Purity and Yield

RNA Oligo Scale of Synthesis and Typical Yield of Unmodified Oligos*

All ssRNA is produced with RNase-free handling and shipped with UV quantitation. Mixed bases may limit MS analysis. For sequences >40–50 nt or high GC/structure, PAGE or dual purification is recommended.

Scale	Crude Desalted			HPLC Purified			Gel Purified
Scale	A₂₆₀ Units	nmols	mg	A₂₆₀ Units	nmols	mg	A₂₆₀ Units	nmols	mg
200 nmol	~15	~75	~0.5	~3	~15	~0.15	~1	~5	~0.04
1 μmol	~40	~200	~1.4	~15	~75	~0.6	~5	~25	~0.2
2 μmol	~72	~360	~2.52	~27	~135	~1.08	~9	~45	~0.36
5 μmol	~160	~800	~5.6	~60	~300	~2.4	~20	~100	~0.8
10 μmol	~320	~1600	~11.2	~120	~600	~4.8	~40	~200	~1.6
15 μmol	~480	~2400	~16.8	~180	~900	~7.2	~60	~300	~2.4
Purity & Notes	Purity: >75% (sequence/structure dependent). HPLC may be recommended for sensitive applications.			Purity: 85–95% (sequence/structure dependent; high-GC may lower yield/purity).			Purity: 95–98%. Yield drops with length; palindromes/hairpins/high-GC or ≥3 G runs reduce purity/yield.

* Values are typical yield of 20 mer oligo; actual yields vary with sequence, length, modifications, and purification.

* 1) Typical yield stated in the table is for unmodified random sequence oligos. Reduction in yield is observed with high GC content oligos and those forming strong secondary structures.

2) Reduced yield is expected with modified oligos. The reduction percentage varies with modification type and number of sites. Typical reduction is 10%-20% per modified site.

3) Yield of 40µg/A260 unit for RNA oligos is calculated for an ~equimolar base composition. Long stretches of a single base or homopolymers will have variable yields. Example for homopolymeric 50 mer: A(50) = ~20/A260 Unit; G(50) = ~28/A260 Unit; U(50) = ~35/A260 Unit and C(50) = ~39/A260 Unit

Request ssRNA Compare Purification

Product	Length	RNA bases	2'OMe RNA	Annealing Fee	RNase-free HPLC
250 nmole scale Duplex	5-60 bases	$10.00/base	$8.00/base	$50	$125/strand
1 umole scale Duplex	5-60 bases	$18.00/base	$15.00/base	$65	$195/strand
2 umole scale Duplex	5-60 bases	$30.00/base	$25.00/base	$100	$250/strand
5 umole scale Duplex	5-60 bases	$62.00/base	$55.00/base	$150	$439/strand
* We offer larger quantity than listed, contact us or request a quote.!

Final Amount	Price (USD)
50 mg	$2,500
100 mg	$3,500
250 mg	$6,000
500 mg	$10,500
1,000 mg	$14,500
>1,000 mg	Inquire

Popular RNA Modifications

2′-O-Methyl (2′-OMe)

Improves nuclease resistance and reduces innate immune signaling.

2′-Fluoro (2′-F)

Enhances stability; often combined with 2′-OMe for siRNA.

Pseudouridine (ψ) / m¹ψ

Base-level modification for reduced immunogenicity and improved translation.

Phosphorothioate (PS)

Terminal or patterned PS for exonuclease protection.

LNA / cEt Locks

Boosts T_m and affinity; strategic placements in guides/aptamers.

Conjugates

GalNAc, cholesterol, tocopherol, PEG, peptides; biotin/dyes/quencher sets.

Dye Labels

Fluorophores: 6-FAM, HEX, TET, JOE, VIC, TAMRA, ROX, Cy3, Cy5, Cy5.5, Cy7, Alexa Fluor 488/555/647, Atto 488/550/647
Quenchers: BHQ-1/2/3, Iowa Black FQ/RQ, DABCYL, Eclipse, BBQ-650
Pairings: FAM–BHQ1, HEX–BHQ1, Cy5–BHQ3, TAMRA–BHQ2
Positions: 5′, internal (base analogs), 3′; spacers HEG/TEG/PEG available

Modifications

Bases: m⁶A, 5-meC, 5-hmC, s²U, ψ / m¹ψ, 2′-OMe, 2′-F, 2'MOE, 2'-F-ANA, LNA/cEt/BNA and much more..
Backbones: PS, PN, boranophosphate, 5′-phosphate/monophosphate
Handles/Spacers: amino/thiol/azide/alkyne; HEG/TEG/PEG linkers
Conjugates/End-caps: GalNAc, cholesterol, tocopherol, peptides, polymer, inverted dT; biotin/dyes

See Full Mod List

RNA in Plates

High-throughput synthesis and delivery in 96- or 384-well plates, lyophilized or in solution, with barcoded labels and downloadable plate maps.

Product	Length	RNA bases	2′OMe Base	Chimeric Base	RNase-free HPLC
100 nmole	10–60	$8.50	$8.00	$0.50	$95
250 nmole	5–60	$10.00	$8.50	$0.70	$130
1 μmole	5–60	$18.00	$15.00	$1.30	$205

Oligo-in-Plate Summary

Formats: 96-well or 384-well (horizontal A1–H12 orientation by default).
Delivery: Lyophilized or in solution; barcoded tubes/plates.
Docs: Downloadable plate maps (CSV/Excel) and COA per lot.
QC: HPLC/PAGE options, LC-MS identity where applicable.
Handling: RNase-free processing; dry-ice or cold-pack shipping.

When placing an order, please indicate the plate map and plate type (e.g., 96-well V-shape) and the desired well volume.

Speak to a Scientist See Modifications

Duplex RNA in 96-well Plate

Duplex RNA supplied in a barcoded 96-well plate for high-throughput workflows.

Long RNA Synthesis (Chemical & Enzymatic)

Bio-Synthesis can produce high-quality long RNA (>100 nt) by solid-phase chemical synthesis. Feasibility and yield are sequence-dependent—secondary structure and certain motifs can reduce coupling efficiency, so yields generally decrease as length increases.

For very long constructs or when chemistry is impractical, we offer enzymatic RNA (IVT) transcription from a DNA template, reaching a few hundred nucleotides to multi-kilobase lengths.

Chemical Long RNA

Practical window: ~80–120 nt (sequence-dependent)
Supports 2′-OMe, 2′-F, ψ/m¹ψ, LNA/cEt, terminal PS (by design)
Purification: PAGE or IEX-HPLC; dual on request

Enzymatic Long RNA (IVT)

Length: ~200 nt to multi-kb (template-dependent)
Template: dsDNA/plasmid; sequence screening before quote
QC options: gel/PAGE or FA/Bioanalyzer; HPLC/PAGE available

About Long RNA

Applications of Long RNA

Long RNA (≈100 nt to multi-kb) enables protein expression, genome editing, regulatory studies, structural biology, and assay calibration. Choose chemical synthesis for precise base-level modifications up to ~80–120 nt (sequence-dependent), and enzymatic IVT for longer constructs.

Application	Typical Length	Examples	Recommended Route	Purification / QC
mRNA expression & vaccines	0.5–5 kb (or more)	Reporter or therapeutic mRNA	IVT (enzymatic)	HPLC/PAGE (dsRNA reduction); gel/Bioanalyzer trace
CRISPR workflows	~98–140 nt	sgRNA, pegRNA, arrays (Cas12/Cas13)	Chemical (≤~120 nt) or IVT	RP/IEX-HPLC or PAGE; LC–MS where applicable
Regulatory & lncRNA studies	0.1–2 kb	5′/3′ UTRs, lncRNA domains	IVT (>~120 nt)	Gel/Bioanalyzer; optional HPLC
Structural & biophysics	0.1–0.5 kb	Riboswitches, ribozymes, folding/SHAPE	Chemical (≤~120 nt) or IVT	PAGE for stringency; IEX-HPLC for charge variants
Standards & controls	0.2–1 kb	qPCR/RT-qPCR standards, NGS spike-ins	IVT	Quant (A₂₆₀); gel/Bioanalyzer; optional HPLC
RNA–protein interaction	0.1–1 kb	EMSA, pulldown, RNP reconstitution	IVT (length-flexible)	PAGE/HPLC as needed; RNase-free handling
Cell-free & synbio	0.5–3 kb	In-vitro translation, RNA scaffolds	IVT	Gel/Bioanalyzer; optional polishing by HPLC/PAGE

Note: Chemical feasibility above ~120 nt is sequence-dependent; yields decrease with length and secondary structure.

Our Long RNA oligo

Bio-Synthesis RNA oligonucleotides are routinely chemical synthesize RNA >120 nt with consistency and superior levels of purity.

Below is an example of 153 nt RNA with calculated calculated MW of 22765.65 and observe M.W of 22766.50

1429 nt Ultra Long RNA by In Vitro Transcript

Request Long RNA Talk to a Scientist

RNA Purification — Add-On Pricing

Typical as-synthesized purity for unmodified ssRNA ≤35 nt is ~80–85% (sequence-dependent). We recommend HPLC or PAGE purification when RNA oligos are:

Longer than ~40–50 nt
Modified internally or at the 3′ end
Dually labeled (e.g., dye/quencher, dual dyes)
Intended for sensitive assays or in vivo use

For recommendations or purity estimates for unpurified material, please contact Scientific Support.

Product Scale	RNase-free PAGE	RNase-free HPLC	RNase-free IE-HPLC	RNase-free Dual HPLC	RNase-free In vivo GF	In vivo RNase-free HPLC	In vivo RNase-free Dual HPLC
100 nmol	$125	$130	$130	$169.0	$228	n/a	n/a
250 nmol	$125	$130	$130	$169.0	$228	$417.00	$521.25
1.0 μmol	$210	$205	$205	$266.5	$288	$550.00	$687.50
2.0 μmol	$310	$325	$325	$422.5	$288	$730.00	$912.50
5.0 μmol	$105	$418	$418	$543.4	$288	$925.00	$1,125.00
10 μmol	$1,900	$632	$632	$821.6	$366	$1,055.00	$1,318.75
15 μmol	Inquire	$832	$832	$1,081.6	$500	$1,415.00	$1,768.75

Notes: Pricing shown per oligo. Final cost may vary with length, chemistry, and requested QC.

Purification Option

Purification and Processing Options for RNA

Desalted: Salts/small molecules removed by ethanol precipitation or C18 column desalting (column desalting is typically used for PAGE-purified RNA and RNA <10 nt).
Deprotected: Base protecting groups have been removed.
PAGE: Purified by polyacrylamide gel electrophoresis.
HPLC: Purified by ion-exchange high-performance liquid chromatography (IEX-HPLC).
In vivo: Processed for in vivo use: sodium (Na⁺) counter-ion exchange, desalting, sterile filtration, and endotoxin testing.
In vivo HPLC: In vivo processing combined with HPLC purification.

Purification and processing options for RNA
Feature	Desalt/Deprotect	PAGE	HPLC	In vivo	In vivo HPLC
Deprotected	✓	✓	✓	✓	✓
Desalted	✓	✓	✓	✓	✓
Endotoxin tested				✓	✓
Sodium counter-ion exchange				✓	✓
Recommended for dually labeled RNA			✓		✓
Recommended for in vivo use				✓	✓

Request Custom RNA

Optional Add-On Services

Custom Formulation & Packaging

Buffers (TE/PBS/custom), aliquoting, concentrations, tubes or plates, OEM labels/barcodes.

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Custom Quality Control

LC-MS, CE, extended HPLC traces, endotoxin/bioburden, water content, residual chemical analysis, stability program.

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Regulatory & OEM

RUO→GLP→cGMP pathways, document packages, tech transfer, sequence masking.

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Need something not listed?

We routinely implement bespoke chemistries and conjugations for RNA.

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Custom RNA Oligo Synthesis - Where chemistry meets confidence.

Custom RNA Synthesis