Precision Bioconjugation for Complex Biomolecules

Advanced, chemistry-driven conjugation integrating linker design, payload attachment, and stoichiometry control across antibodies, proteins, peptides, and oligonucleotides—supported by LC-MS/HPLC analytics and ISO 9001/13485 quality.

Site-specific options
Click (CuAAC/SPAAC/IEDDA)
Maleimide-thiol • Oxime
RUO → GLP/GMP-like

Overview

Bio-Synthesis Inc. specializes in site-selective and multifunctional conjugation across a diverse range of biomolecules. We delivers custom bioconjugation across antibodies, proteins/peptides, oligonucleotides,small molecule, hapten and drug compound. We engineer linkers (cleavable/non-cleavable PEG, VC-PAB, disulfide, oxime/hydrazone) and run bioorthogonal chemistries (CuAAC, SPAAC/DBCO–Azide, IEDDA/TCO–Tetrazine) with control of substitution and site. Integrated analytics (LC-MS, UPLC/HPLC, SEC-MALS, DLS) and ISO 9001/13485-aligned documentation support scale-up from mg to kg for diagnostics and therapeutic research.

Scale
mg → g → kg
QC
UPLC/HPLC • LC‑MS • SEC‑MALS • DLS
Supply
RUO → GMP‑like (U.S. facilities)

Service Capability

We design, execute, and qualify conjugations across biomolecules, chemistries, and formats—paired with robust analytics, documentation, and scale‑up so your materials are ready for research use and regulated pathways.

Biomolecules We Conjugate
  • Antibodies & Fragments (IgG, Fab, scFv, VHH)
  • Oligos (DNA, RNA, siRNA, ASO, aptamers)
  • Peptide, Proteins & Enzymes
  • Lipids & Liposomes
  • Nanoparticles
  • Small molecules& haptens
Conjugation Chemistries
  • NHS/amine, maleimide/thiol, EDC/sulfo‑NHS
  • Click: CuAAC, SPAAC, IEDDA
  • Carbonyl: oxime/hydrazone, reductive amination
  • Photo & enzymatic coupling
Payloads & Labels
  • Fluorophores, biotin, digoxigenin
  • Enzyme labels (HRP, ALP)
  • Drugs, chelators (DOTA, NOTA, DTPA)
  • Photocages, photoswitches, MRI agents
Linkers & Spacers
  • Cleavable: disulfide, VC-PAB, hydrazone
  • Non-cleavable: triazole, thioether
  • PEGylation (linear/branched)
  • Stimuli-responsive & custom length
Site‑Specific Conjugation
  • Engineered cysteines, glycan remodeling
  • Unnatural AAs (azido, alkyne, ketone)
  • Enzymatic tags (LPXTG, Q‑tag, FGE)
  • Controlled DAR/labeling ratio
Surfaces & Solid‑Phase
  • Beads & microspheres (magnetic, latex)
  • Biosensor chips (SPR/BLI)
  • Microarrays & membranes
  • Device coatings (RUO)
Carriers & Delivery
  • Liposomes, polymersomes
  • Polymeric & inorganic nanoparticles
  • Hydrogels, scaffolds
  • Targeting ligand display
Development & Scale‑Up
  • Feasibility & design consulting
  • Pilot → process development
  • Scale‑up (mg → kg)
  • GLP / GMP‑like production
Analytical Characterization
  • LC‑MS, MALDI‑TOF, HPLC/UPLC
  • SEC‑MALS, DLS, UV/Vis
  • ELISA, SPR/BLI, activity assays
  • Stability, endotoxin, sterility
Formulation & Packaging
  • Buffer optimization & exchange
  • Stabilizer screening
  • Sterile filtration, lyophilization
  • Custom vialing, labeling
Documentation & QA
  • CoA, batch records, traceability
  • Method validation, change control
  • ISO 9001/13485 compliance
  • Secure LIMS, IP protection
Application Areas
  • Diagnostics (ELISA, LFIA, flow, IF)
  • Therapeutic research, ADC prototypes
  • Imaging (optical, PET/SPECT, MRI)
  • Biosensors, omics workflows

Chemistry Platforms We Run Routinely

Orthogonal, high-yield coupling strategies optimized for activity preservation, site control, and clean analytics.

Click Chemistry
  • CuAAC (alkyne–azide) with copper management to protect proteins/oligos.
  • SPAAC (DBCO–Azide) copper-free for sensitive biomolecules & live-cell use.
  • IEDDA (TCO–Tetrazine) ultra-fast; ideal for site-specific handles.
  • Orthogonal protection to control substitution and avoid cross-reactivity.
Thiols & Carbonyls
  • Maleimide/Thiol and haloacetyl coupling for cysteine or engineered sites.
  • Oxime/Hydrazone from aldehyde/ketone handles (incl. glycan engineering).
  • Reductive amination with tight pH and stoichiometry control.
  • Fc glycan remodeling options for antibody conjugation.
Enzymatic & Photocontrolled
  • Sortase A (LPXTG), Transglutaminase, and FGE aldehyde-tag workflows.
  • Photocages/photoswitches for spatiotemporal release or activation.
  • Placement precision with preserved biological function.
  • Compatible with PEG, cleavable linkers (VC-PAB, disulfide) and chelators.

Our Collaborative Process

A transparent, science-first development cycle integrating design, feasibility, optimization, analytical qualification, and scale-up—streamlined for reproducibility and speed.

1
2
3
4
5
1
Design
Define target, reactive site(s), and conjugation strategy with chemistry mapping and payload selection.
2
Feasibility
Perform micro-scale conjugations and stability checks to validate linker compatibility and efficiency.
3
Optimization
Adjust stoichiometry, pH, solvent/buffer composition, and order of addition for yield and reproducibility.
4
Qualification
Comprehensive analytical release (LC-MS, UPLC/HPLC, SEC-MALS, DLS, activity) ensuring conjugate integrity.
5
Scale-Up
Process transfer from R&D to production, mg→g→kg scale with full documentation and QA review.

Where Our Conjugates Are Used

Diagnostics

Immunoassays (ELISA, CLIA), lateral-flow labels, flow cytometry, IF/IHC, nucleic-acid detection probes, and QC controls.

Therapeutic Research

Prototype ADCs/AOCs, targeted delivery, receptor occupancy, PK/PD tracking, and payload/linker evaluation.

Imaging & Sensing

Optical and nuclear imaging (PET/SPECT) via chelators (DOTA/NOTA/DTPA), ECL/redox tags for electrochemical biosensing.

Ready to start your conjugation project?

Share your target, desired payload/label, and scale. Our scientists will respond with a feasibility plan and timeline.

Request a Quote

Speak to a Scientist

Tell us about your biomolecule, desired label/payload, and intended use. We’ll recommend chemistry, linker design, and release testing to meet your goals.

Full Name *
Email *
Company / Institution *
Phone *
Desire Scale
Project Details

By submitting, you agree to be contacted regarding your request.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 40+ years of delivering high quality, fast and scalable synthetic biology solutions.