Custom long DNA synthesis for advanced molecular biology, CRISPR, and synthetic biology workflows, with sequence lengths beyond standard 200-base oligos.
Long DNA synthesis enables access to extended single-stranded DNA constructs for applications such as genome engineering, synthetic biology, and advanced molecular workflows [1], [2].
Bio-Synthesis offers DNA Ubermer long DNA synthesis up to 400 nt with typical quantities ranging from µg to several mg, depending on oligo length, sequence composition, and synthesis strategy. This service supports research programs that need extended ssDNA or dsDNA with flexible sequence design, optional modifications, purification, and analytical review.
Our long DNA workflow is designed to support demanding constructs with attention to sequence complexity, manufacturability, and fit-for-purpose QC.
Long DNA synthesis concept. Extended DNA constructs produced for applications including genome engineering, CRISPR workflows, and synthetic biology design.
Maximum length
Single & double strand from µg to mgs
Sequence-by-sequence design
Modification options
Purification and analysis
Support DNA designs that exceed conventional oligonucleotide ranges.
Tailor sequence, modification, and purification to your workflow.
Suitable for molecular biology, CRISPR, and synthetic biology programs.
Submit single long DNA targets or broader design panels for research-driven development workflows.
Long DNA constructs can be designed with project-aligned modification strategies subject to sequence feasibility.
Purification and analytical review can be matched to sequence complexity and downstream application requirements.
DNA Ubermer long DNA synthesis up to 400 nt • project-specific design review • selected modification support • application-aligned purification • analytical characterization • technical consultation during quoting and planning.
DNA Ubermer supports extended-length DNA constructs beyond standard oligonucleotide ranges. Final specifications depend on sequence design, complexity, and project requirements.
DNA Ubermer is positioned for projects that need longer DNA sequence length than conventional long oligonucleotide products, supporting up to 400 nt with broad modification compatibility for advanced research workflows.
DNA Ubermer supports a broad set of long-DNA modification options commonly requested for cloning, donor design, control templates, labeling, and specialized assay development workflows.
Available options can include 5′ phosphorylation, 5′ biotin, 5′ amino modifiers, 5′ deoxyInosine, 5′ deoxyUridine, and related project-specific formats.
Internal deoxyInosine, deoxyUridine, 5-methyl dC, and other sequence-specific base modifications can be incorporated based on design compatibility.
Phosphorothioate linkages and other backbone-oriented changes can be used to support stability and specialized functional requirements.
2′-O-methyl RNA bases and selected ribo-base options may be incorporated when hybrid DNA/RNA design is needed for a specific application.
3′ amino modifiers, 3′ phosphorylation, 3′ spacers, and other terminal modifications can be aligned with downstream assay or construct design needs.
Spacer 18 and related structural elements can be used to tune construct architecture, reduce steric effects, or support custom molecular formats.
DNA Ubermer is intended to support the standard long-DNA modification categories commonly requested in the market, while extending sequence length beyond typical long-DNA oligo offerings. Final compatibility depends on sequence composition, modification placement, and synthesis feasibility.
Long DNA constructs can be prepared using different synthesis strategies depending on sequence length, design complexity, modification requirements, and downstream application.
Enables the production of sequence-defined long DNA oligonucleotides through solid-phase chemical synthesis, supporting controlled design, selected modifications, and flexible research use.
Longer DNA constructs can also be generated using ligation chemistry, where smaller sequence segments are assembled into a larger product to address challenging designs or extended length requirements.
The optimal synthesis path depends on target length, sequence composition, requested format, modification placement, and intended application. Our team can recommend the most suitable approach during project review.
Direct chemical synthesis is typically preferred for well-defined long DNA constructs with controlled sequence requirements, while ligation-based strategies may be used when construct design, length, or assembly considerations make a segmented approach more appropriate.
Our team can review target length, sequence complexity, modification requirements, purification preferences, and intended research application to recommend an appropriate long DNA synthesis approach.
DNA Ubermer is Bio-Synthesis long DNA synthesis for custom ssDNA constructs up to 400 nt for research use.
DNA Ubermer supports custom long DNA constructs up to 400 nt, depending on sequence design and project requirements.
Yes. Selected modification and labeling options can be discussed during project review and are assessed based on sequence feasibility and intended use.
Please provide the DNA sequence, target length, scale, modification requirements, purification preferences, and intended application.
Share your target sequence length, modification needs, purification requirements, and intended application. Our team will help align your design with the most suitable synthesis strategy and workflow.
Rapid feasibility review • Project-based support • Research-focused solutions
Selected references related to long DNA oligonucleotide synthesis, genome engineering, synthetic biology, and advanced molecular applications.
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