Overlapping peptide libraries, peptide pools/subpools, and mapping panels for antibody and T-cell epitope identification — with defined specs and documented QC. Capabilities: mg–gram scale per peptide, crude/≥95%/≥98% purity tiers, and optional HPLC/UPLC + LC‑MS verification (sequence‑dependent).
Epitope mapping identifies which region(s) of an antigen are recognized by an antibody or immune receptor. It is commonly used to characterize specificity, localize binding regions, and guide follow-on work such as antibody engineering, assay development, or vaccine research [1].
A practical starting point is distinguishing linear epitopes (continuous sequence segments) from conformational epitopes (residues brought together by protein folding). Overlapping peptide libraries and follow-on scan panels are especially useful for localizing linear epitopes and refining minimal motifs [4].
For epitope selection context and standardized identifiers, researchers often reference the Immune Epitope Database (IEDB) and NIAID’s IEDB overview [1], [2].
Related pages: Peptide pools, Overlapping peptide libraries, and Vaccine peptides.
Overlapping peptides to localize binding regions, then truncations/scan panels to define minimal motifs.
Variant panels and comparative sets to assess cross-reactivity and binding sensitivity.
Overlapping peptide sets and pools/subpools for screening and deconvolution workflows.
Fixed-length overlapping libraries and curated pools aligned to ELISpot/ICS readouts.
WT vs variant mapping panels to compare binding or immunogenicity in strain/variant contexts.
Positive/negative controls, spacer/linker options, and case-by-case formatting for downstream platforms.
Mapping projects rank well when they are explicit about specs, QC packages, and scale tiers. Use the table below as a clear “what we can deliver” summary for quote-ready programs.
If you’re exporting from IEDB, include IDs or exported lists to reduce reformatting [1].
Design and synthesize tiled libraries (length/overlap defined) for mapping and deconvolution workflows.
Pool composition options for screening, plus subpool formats to localize signal efficiently.
Epitope peptides, long peptides, and pool-ready formats for immunology research workflows.
Custom neoantigen peptides and panels for preclinical research pipelines (project-defined specs).
PTM-mimics and stability modifications to support binding studies and method development.
FASTA/CSV/Excel inputs, named controls, and consistent formatting for repeat orders.
Library format strongly impacts resolution, total peptide count, and downstream screening efficiency. In addition to custom manual design, Bio-Synthesis provides peptide library design software to assist with overlap schemes, truncation panels, and screening layouts. View Peptide Screening Tools.
Common peptide library strategies: overlapping libraries, truncation panels, alanine scanning, PTM arrays, T-cell peptides, and mutation panels.
Fixed-length peptides tiled across a target region (e.g., 15-mers with 10-aa overlap) to localize linear epitopes and define binding regions.
Explore Overlapping Peptide Library Design→
Systematic N- and C-terminal truncations to refine minimal epitope motifs following initial library screening.
Learn more about Truncation Panels →
Single-residue substitutions (commonly alanine) to identify key binding determinants and critical contact residues.
View Alanine Scanning Methods →
Class I and Class II compatible peptide sets formatted for ELISpot/ICS and cellular immune screening workflows.
Learn more about T-cell Peptide Libraries →
Parallel panels comparing unmodified vs post-translationally modified (PTM) peptides to assess PTM-dependent binding.
See PTM Peptide Arrays →
Wild-type vs variant peptides to evaluate cross-reactivity, strain differences, and mutation-driven binding changes.
Learn more about Mutation Panels →
Project-defined confirmation steps can be applied before pooling (e.g., LC-MS/HPLC checks where feasible).
Defined lists, traceable handling, and pool/subpool design choices help reduce ambiguity and support reproducibility.
Marker peptide confirmation can be discussed for select pools based on pool size, assay sensitivity, and acceptance criteria.
Pool QC approaches depend on pool size, peptide properties, and intended assay. Include your pool format and acceptance criteria in the quote request.
Send sequence/region and readout (binding/ELISA/ELISpot/ICS) plus desired resolution.
Pick length/overlap, add controls, and define variants/truncations as needed.
Screen initial library (individual or pooled), then refine with scan/truncation panels.
Analytical HPLC/UPLC purity profile and LC-MS intact mass confirmation (when feasible).
CoA and project-defined documentation (sequence list, pool composition record, naming conventions).
Traceable lists and consistent formatting help repeat orders match confirmed epitope reagents.
Screening libraries often use crude or ≥95% depending on assay sensitivity and peptide properties. Confirmatory panels commonly specify ≥95% or ≥98%. Define acceptance criteria in your quote request.
A set of peptides that tile across a protein/region with a defined overlap to localize binding regions and support refinement to minimal motifs.
A common approach uses a 5-aa step (15 length minus 10 overlap). Provide the region and we can format the library list.
Yes. If available, include IEDB IDs/exports and any strain/variant context. [1], [2]
Yes. Pools reduce handling; subpools help localize signal and simplify deconvolution. Pool verification options are available case-by-case.
Yes. PTM arrays (unmodified vs modified) and other project-defined modifications can be incorporated when you need to evaluate modification-dependent binding or method development.
Choose based on assay and resolution needs. A common scheme is 15-mers with 10-aa overlap (5-aa step), then follow-on truncations or fine-step panels to define minimal motifs.
Conformational epitopes may require structure-informed approaches. Constrained/cyclic peptide designs or targeted panels can be discussed depending on the project.
A peptide pool combines multiple peptides into a single mixture for efficient screening. Subpools split a large pool into smaller sets so you can localize signal faster before testing individual peptides.
Initial screening libraries are commonly supplied at mg-scale per peptide. Confirmatory peptides and resupply lots can be scaled as needed (sequence-dependent) once targets are prioritized.
Share your design details and timeline. We’ll recommend a feasible library plan and an appropriate analytical characterization package.
Links are provided for scientific background; access may depend on institutional subscriptions.
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