5‑fluoro, 5‑bromo, and 5‑iodo analogs of U/C (DNA & RNA) for UV photo‑crosslinking, heavy‑atom phasing, duplex tuning, and enzyme/kinetic studies — synthesized, purified, and QC’d to your specs, scalable to ISO 13485.
Bio‑Synthesis provides custom halogenated oligonucleotides—DNA and RNA modified with 5‑bromo, 5‑iodo, or 5‑fluoro pyrimidines (U/C)—for UV photo‑crosslinking, X‑ray crystallography heavy‑atom phasing (MAD/SAD), duplex Tm tuning, and enzyme/kinetic studies. These halogenated bases preserve native pairing while adding photochemical or heavy‑atom functionality, making them ideal for crosslink mapping, structural biology, and biophysics workflows.
We deliver site‑specific placement at the 5′, 3′, or internal position (including spacer/PEG options), with scales from nmol to multi‑gram. Every lot is purified (HPLC/PAGE) and identity‑confirmed by ESI‑MS, with optional endotoxin and documentation for ISO 9001/13485 programs. Related services include custom probe design for photo‑crosslinking assays, biotin/ enzyme conjugation, and diagnostic oligos—all supported by experienced scientists. Ready to plan your construct? Use Speak to a Scientist for fast, expert guidance.
Ideal for crosslink mapping, structural biology, and tuning duplex energetics, these 5‑position halogenated pyrimidines are available at desired sites and scales with HPLC/MS QC and optional functional testing.
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Design help for crosslink mapping and structural assays.
Heavy-atom + affinity capture combinations for workflows.
Usually small shifts. Verify your probe length/salt to maintain desired Tm and specificity.
5‑I provides stronger absorption/heavy‑atom effects but can be less stable; 5‑Br is a good balance for UV crosslinking. Pilot both when possible.
Store dry and protected from light; avoid prolonged UV exposure prior to use. Standard –20 °C recommended.
We’ll recommend base/position, purification, and assay conditions to match your workflow.
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