High-quality large-scale oligonucleotide synthesis with advanced modifications from milligrams to 100+ grams. Rigorous QC documentation to accelerating programs from discovery to clinic.
Large-scale oligonucleotide synthesis is the production of DNA and RNA oligos at milligram to multi-gram quantities for research, diagnostic, industrial, and therapeutic applications. Compared with small-scale oligo synthesis, large-scale manufacturing requires optimized chemistry, scalable purification, and strict quality control to ensure consistency and reproducibility.
Bio-Synthesis provides large-scale DNA and RNA oligonucleotide manufacturing with flexible scale-up, advanced modification capabilities, conjugation options, purification, and full analytical QC support — enabling transition from discovery and feasibility through preclinical and therapeutic development programs.
What you can configure
Large-scale oligonucleotide synthesis involves sequence design, feasibility assessment, process scale-up, controlled manufacturing, and analytical QC release. Scaling from milligram to gram quantities requires optimization of synthesis chemistry, purification strategy, and impurity control to ensure reproducibility and batch consistency.
Large-scale oligo programs can be configured by oligo type, sequence length, chemistry, chemical modification pattern, conjugation strategy, purification grade, delivery format, scale, documentation, and QC requirements. Production ranges from milligram research batches to gram, multi-gram, and 100+ gram manufacturing for qualified programs.
This workflow outlines key stages of large-scale oligonucleotide synthesis, from sequence evaluation through scale-up, manufacturing, and QC release.
Scale
100 g+
Large-batch capability for qualified DNA/RNA projects
Oligo types
DNA / RNA
ASO, siRNA, primers, probes, modified oligos
Chemistry
Modified
PS, PN, 2′-OMe, 2′-F, LNA/BNA, Morpholino and conjugates
QC
QC & Documentation
Purity and impurity control, identity, CoA, traceability for reulatory pathway planning
Small-scale oligo synthesis is typically used for early research and screening. Large-scale production is designed for projects that require higher mass, controlled purification, batch reproducibility, and more extensive documentation.
At Bio-Synthesis, our manufacturing platform is designed to support projects ranging from milligrams to 100+ grams of DNA and RNA oligonucleotides. We combine high-throughput instrumentation, diverse chemistry portfolios, and GMP-aligned workflows to ensure consistent quality at every scale.
Research-grade to cGMP with consistent chemistry and documentation.
*Sequence-dependent capability.
Chemistries for stability, delivery, and function.
Regulatory-ready data packages.
Optional tests: endotoxin, bioburden, residual solvent, heavy metals, and custom analytical methods are available on request.
Large-scale oligonucleotide projects often require chemical modifications to improve nuclease resistance, binding affinity, delivery, detection, or biological performance. Bio-Synthesis supports a broad range of modification and conjugation strategies for DNA and RNA oligos.
Purification strategy is selected based on oligo length, chemistry, scale, conjugation, intended use, and required purity.
QC packages can be configured to support RUO, GLP-aligned, or cGMP-oriented development workflows.
Quality-focused scale-up
Large-scale oligo manufacturing requires more than simply increasing batch size. Each scale-up program should evaluate synthesis yield, impurity profile, purification method, analytical release criteria, storage conditions, and documentation expectations before moving into routine supply or development-stage production.
Support for siRNA, ASO, gapmer, splice-switching oligo, modified RNA, and conjugated oligonucleotide programs.
Large-scale primers, probes, molecular beacons, FRET probes, adapters, barcoded oligos, and assay components.
Indexed primers, adapters, capture probes, UDI libraries, and sequencing workflow oligos.
Guide RNAs, donor oligos, capture probes, and specialty oligos for genome editing and synthetic biology workflows.
Bulk oligo supply, private-label support, OEM labeling, long-term supply agreements, and custom packaging.
Scale-up support from feasibility batches to larger development lots with expanded documentation and QC.
Large-scale oligonucleotide manufacturing requires controlled processes, validated workflows, and rigorous quality systems. Bio-Synthesis supports projects from research-grade synthesis through development-stage and cGMP-oriented production with full traceability and documentation.
Scalable workflow from research to development and clinical support.
Bio-Synthesis supports large-scale oligonucleotide programs with chemistry expertise, flexible project configuration, manufacturing experience, and documentation designed to support reproducibility and long-term supply.
From feasibility and pilot batches through development-scale production, our team helps align synthesis, purification, QC, and documentation with your application requirements.
Custom siRNA synthesis with flexible formats, advanced modifications, delivery conjugates, and scalable production support.
Antisense oligonucleotide synthesis with backbone, sugar, terminal, and conjugation options for research and development.
Custom oligonucleotide conjugation services including GalNAc, lipid, peptide, PEG, dye, ligand, and reactive-handle strategies.
To request a large-scale oligonucleotide quote, provide the sequence, oligo type, desired scale, modification requirements, purification method, QC expectations, and intended application. If the final configuration is not yet defined, our team can help review feasibility, scale-up strategy, and documentation needs.
If your program is moving from small-scale synthesis to development or manufacturing scale, Bio-Synthesis can help evaluate:
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