Research Use Only (RUO) Peptides

High-purity synthetic peptides for laboratory research — supporting assay development, receptor biology, immunology, mechanism studies, and discovery workflows with clear analytical documentation.

RUO Only Custom Synthesis (SPPS) Modifications & Labels HPLC / MS QC CoA & Analytics ISO 9001:2015 QMS

ISO 9001:2015 Quality Management System: RUO peptide operations are conducted under an ISO 9001:2015 certified quality management system ensuring documented processes, traceability, and continuous improvement.

Overview

What Are RUO Peptides?

Research Use Only (RUO) peptides are synthetic peptide products intended strictly for laboratory research. They are commonly used in in vitro experiments, preclinical studies, assay development, and exploratory biology programs.

RUO peptides are supplied with analytical verification appropriate for research workflows (e.g., HPLC purity and MS identity), plus documentation such as a Certificate of Analysis (CoA). Specifications can be tailored to your application.

RUO peptide manufacturing and release processes operate under an ISO 9001:2015 certified quality management system.

RUO peptide workflow

Single-view summary from requirements to QC + documentation — designed to keep projects
moving without clutter.

ISO 9001:2015 QMS
Define specs

Sequence, purity, scale, salt form, and any labels/handles.

Specs
Synthesize

SPPS build with route planning for difficult sequences.

SPPS
Purify

Desalt and/or prep HPLC to meet target purity.

Purify
QC verify

HPLC/UPLC purity + MS identity confirmation.

QC
Deliver

Lyophilized peptide + CoA and analytical outputs.

COA
Fit-for-Purpose Specs

Choose purity, scale, salt form, and analytics aligned to your assay sensitivity and intended research use.

Reproducible QC

Batch-level purity and identity confirmation (HPLC/MS) with documentation to support repeatable results.

Fast Iteration

RUO synthesis supports rapid design-test cycles for SAR series, variants, libraries, and optimization studies.

Intended Use
  • For laboratory research use only.
  • Not for diagnostic procedures.
  • Not for therapeutic use or human administration.
Practical Note
  • Specify your application (binding assay, ELISA standard, in vivo model) so we can recommend practical purity/analytics.
  • For clinical/regulated programs, request GMP pathways instead of RUO.

Features & Benefits

Built for reproducible research: robust synthesis, flexible modification support, and transparent analytical reporting — without the overhead of clinical-grade programs.

Reliable Quality

Controlled SPPS workflows with batch-level identity and purity checks to support consistent experimental outcomes.

Customization Ready

Sequence optimization support plus a broad menu of modifications, labels, linkers, and formats.

Built-In Flexibility

Linear, cyclic, stapled, labeled, PEGylated, or conjugation-ready peptides — tailored to your application needs.

Easy Ordering

Quote-to-delivery support with feasibility review, clear timelines, and rapid communication.

Fast Turnaround

Accelerated production options available (sequence/complexity dependent) for urgent timelines.

Documentation You Can Use

CoA plus analytical outputs (e.g., HPLC trace, MS) to simplify publication, QC, and repeatability.

RUO Peptide Capabilities

Custom Peptide Synthesis

Sequence-defined peptides with research-grade documentation and application-aligned specifications.

  • Linear and cyclic peptides
  • Fragments and truncations
  • SAR series and variants

Explore Custom Peptide Synthesis →

Difficult Sequences

Strategies for hydrophobic, long, cysteine-rich, aggregation-prone, or highly charged peptides.

  • Route/scaffold planning
  • Protecting group strategy
  • Solubility planning

See Difficult Peptide Synthesis →

Modifications & Labels

Make peptides assay-ready with functional handles and labels.

  • N-terminus acetylation / C-terminus amidation
  • Biotin / dye labeling
  • Phosphorylation, PEGylation, lipidation

Explore Peptide Modifications and Labeling →

Required
  • Sequence (one-letter code) and length
  • Scale (mg or g) and purity target
  • Modifications/labels (if any)
Helpful
  • Intended use (assay type, in vivo vs in vitro)
  • Solubility constraints / buffer preferences
  • Special residues (Cys, Met, Trp) or sensitivity (light/oxidation)

Typical RUO Specifications

Common Order Parameters)
  • Purity: typically ≥95% (higher available on request)
  • Scale: mg to multi-gram (sequence dependent)
  • Format: lyophilized powder (default) or custom fill
  • Salt form: standard or custom counter-ion options
Common Add-Ons
  • Extra purification: preparative HPLC
  • Desalting: where appropriate for shorter/clean sequences
  • Labeling: biotin/fluorophores/quencher pairs
  • Conjugation handles: azide/alkyne/maleimide-ready

Tip: If your workflow is quantitative or low-background sensitive, ask for higher purity and additional analytical notes.

Analytics & Quality Control (QC)

RUO peptide performance depends on identity and purity. Analytics should be chosen based on your application (screening vs quantitative assays vs sensitive binding/functional readouts).

Quality System: Research Use Only (RUO) peptide operations are conducted under an ISO 9001:2015 certified quality management system, supporting documented processes, traceability, and continuous improvement.

Standard RUO Release
  • HPLC purity profiling
  • Mass spectrometry (MS) identity confirmation
  • Certificate of Analysis (CoA)
Optional Testing (Request as Needed)
  • Counter-ion analysis / exchange
  • Water content / hygroscopicity notes
  • Stability screening (application-dependent)

If you are comparing lots over time, tell us your QC expectations (acceptance criteria, assay sensitivity, and lot-to-lot constraints).

RUO Peptide Applications

Application context determines recommended purity, modifications, analytics, and handling. Common RUO use cases include:

  • Receptor binding & pharmacology (agonists/antagonists, fragments)
  • Enzyme substrates (cleavage/kinetics, reporters)
  • Assay development (standards, controls, competitive assays)
  • Immunology (epitope mapping, peptide antigens)
  • Drug discovery (SAR series, variants, libraries)
  • In vivo models (exploratory PK/PD research)

Need help selecting labels or conjugation handles for your assay? Use the “Speak to a Scientist” option below.

Handling, Solubility & Storage

Many issues arise after delivery—during reconstitution, storage, or repeated freeze–thaw cycles. Handling guidance is especially important for hydrophobic peptides, charged sequences, and labeled peptides.

Recommended Practices
  • Store lyophilized peptide at −20°C or below (sequence dependent)
  • Aliquot after reconstitution to reduce freeze–thaw cycles
  • Protect light-sensitive labels from exposure
Common Pitfalls
  • Oxidation (Met/Trp) and disulfide handling (Cys)
  • Aggregation with hydrophobic sequences
  • Hygroscopic uptake from ambient humidity

If solubility is critical, tell us your buffer/solvent constraints so we can advise on salt form and reconstitution strategy.

FAQ

What does “RUO” mean for peptides?

RUO means Research Use Only. These peptides are intended for laboratory research and are not intended for diagnostic procedures or therapeutic use.

What purity should I choose?

For many research assays, ≥95% is common. Quantitative assays, low-background systems, or sensitive binding studies may benefit from ≥98% or higher depending on the sequence and use case.

What analytics are typically provided?

Standard RUO release typically includes HPLC purity and MS identity confirmation, plus a Certificate of Analysis (CoA). Additional testing can be added on request.

Can you make cyclic, stapled, or labeled peptides?

Yes. We support a range of structures and labels, including cyclization, dye/biotin labeling, and functional handles for conjugation—availability depends on sequence chemistry and scale.

Can RUO peptides be scaled later?

Often, yes. If you expect future scale-up, mention it early so we can recommend a path that supports continuity (specs, analytics, and risk management).

How should I store my peptide?

Store lyophilized peptide at −20°C or below and minimize moisture exposure. After reconstitution, aliquot and avoid repeated freeze–thaw cycles. Specific guidance depends on sequence and labels.

CONTACT

Speak to a Peptide Scientist

Share your sequence (or target region), intended application, purity/scale needs, and required modifications. We’ll recommend practical specifications and provide a quote.

Request a Quote Contact Form

Tip: If the peptide is hydrophobic/long/cysteine-rich or includes sensitive labels, mention it so we can route the request to the right synthesis strategy.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.