Custom RNA oligonucleotides synthesized in 96-well plate format for RNAi screening, siRNA duplexes, antisense RNA, assay development, probe design, and high-throughput molecular biology workflows.
High-throughput RNA plate oligo synthesis enables parallel production of multiple RNA sequences in standardized 96-well plate formats for scalable, reproducible, automation-ready workflows.
Bio-Synthesis supports custom RNA oligo plates in single-stranded RNA and duplexed RNA formats. This service is designed for researchers who need organized RNA sequence panels, siRNA duplexes, RNAi screening sets, modified RNA libraries, or assay-ready RNA oligos delivered in a defined plate map.
RNA plate oligos may be shipped dry or resuspended to your specifications, with options for standard RNA bases, 2'-OMe, 2'-F RNA, RNase-free HPLC purification, ESI-MS QC, and project-specific modification strategies.
This service supports 96-well RNA oligo plates, RNA plate synthesis, siRNA plate libraries, duplex RNA plates, and high-throughput RNA oligonucleotide production for screening and assay development. Researchers working with antisense oligonucleotides (ASO) can also request modified RNA chemistry and application-specific QC options.
High-throughput RNA plate oligo synthesis workflow. Automated liquid handling and 96-well plate formatting enable scalable and reproducible RNA oligo production.
RNA plate format
Minimum oligos or duplexes
Single-stranded plates
Annealed RNA pairs
HPLC option
Support siRNA, RNAi, and RNA sequence panels with well-by-well tracking and scalable plate formatting.
Add stability-oriented RNA chemistry options where compatible with the sequence and application.
Select RNase-free HPLC and ESI-MS QC options to match downstream research needs.
High-throughput RNA oligonucleotide synthesis is based on solid-phase phosphoramidite chemistry, adapted for RNA-specific 2'-hydroxyl protection strategies. RNA synthesis typically employs 2'-O-protecting groups such as TBDMS or TOM to ensure selective chain elongation and stability during synthesis cycles.
Compared to DNA, RNA oligonucleotides require more stringent handling conditions due to increased susceptibility to hydrolysis and RNase degradation. Post-synthesis processing includes deprotection, cleavage, and optional purification such as RNase-free HPLC.
Modified RNA chemistries such as 2'-O-methyl (2'-OMe) and 2'-fluoro (2'-F) enhance nuclease resistance, improve stability, and are widely used in RNAi, antisense therapeutics, and functional genomics studies.
Individual RNA oligos arrayed into 96-well plates for antisense RNA, probe development, guide RNA components, RNA controls, and assay panels.
One oligo pair per well, annealed and delivered in plate format for siRNA, RNAi screening, and duplex RNA workflows.
RNA base, 2'-OMe, and 2'-F RNA options help support stability, nuclease resistance, and specialized assay designs.
Plate delivery options
Single-stranded RNA oligos may be shipped dry or resuspended to your specifications. Duplexed RNA plates contain one annealed oligo pair per well and are shipped dry unless otherwise arranged during quoting.
The workflow is designed to convert RNA sequence lists and plate layouts into organized, ready-to-use RNA oligos for high-throughput experiments.
Submit RNA sequences, desired scale, plate format, strand format, chemistry type, and delivery preference.
Each sequence or duplex pair is mapped to a specific well position for traceability and automation compatibility.
RNA oligos are synthesized using automated solid-phase chemistry with RNA-compatible protection and deprotection workflows.
Completed RNA oligos are cleaved, deprotected, and processed using RNA-aware handling practices.
RNase-free HPLC and ESI-MS QC options can be selected depending on project requirements.
Final RNA oligos are delivered in labeled 96-well plates with organized plate-map support.
Select a pricing tab below for single-stranded RNA or duplexed RNA in 96-well plate format.
RNA plate oligos can be configured with standard RNA bases, stability-enhancing sugar modifications, terminal labels, and reactive handles depending on sequence design and application.
Useful for RNA controls, RNAi-related studies, hybridization assays, and sequence-defined RNA panels.
A common stability-oriented RNA modification for nuclease resistance and assay optimization.
Supports modified RNA designs requiring improved stability or specialized binding behavior.
Useful for capture, pull-down, immobilization, and detection workflows.
Fluorophore-labeled RNA oligos can support probe, imaging, and assay development applications.
Amino, thiol, azide, alkyne, and other handles may be reviewed for project-specific RNA conjugation strategies.
RNA and DNA plate oligo synthesis both support high-throughput, plate-map-based workflows, but RNA requires additional chemistry controls, RNase-aware handling, and application-specific purification choices.
Choose RNA plate synthesis when biological function requires RNA or modified RNA chemistry. Choose DNA plate oligos when the workflow requires primers, DNA probes, adapters, or more cost-effective DNA screening panels.
Our team can review sequence length, composition, modified bases, duplex design, and purification requirements before production.
Choose single-stranded RNA plates or annealed duplex RNA plates for screening and assay workflows.
Select purification and QC options that align with your downstream RNA workflow and quality expectations.
Explore additional RNA oligonucleotide synthesis services including custom RNA production, large-scale manufacturing, therapeutic oligos, and specialized RNA chemistries.
Yes. RNase-free HPLC is available as a purification option for RNA plate oligos.
A minimum of 24 oligos or duplexes is required for 96-well plate orders. Some configurations may require 96 oligos.
Yes. 2'-OMe and 2'-F RNA options are available depending on sequence design, length, and project feasibility.
Yes. Duplexed RNA plates contain one annealed oligo pair per well. A $256.00 USD annealing fee applies per plate.
Yes. Common RNA modifications and functional groups can be reviewed upon request, including 2'-OMe, 2'-F, biotin, fluorescent dyes, amino, thiol, azide, and alkyne groups.
A 96-well RNA oligo plate contains up to 96 individual RNA sequences or duplex pairs arranged in a standardized format for high-throughput workflows and automation.
RNA plate synthesis is used for siRNA screening, RNAi studies, antisense research, probe development, modified RNA libraries, and high-throughput molecular biology applications.
Single-stranded RNA and duplexed RNA oligos are available in 96-well plate format.
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