Scalable single-stranded and double-stranded DNA production with advanced DNA chemistry options, purification, release QC, documentation, and process-development support from feasibility through scale-up.
Bio-Synthesis provides large-scale DNA synthesis through an integrated manufacturing platform that combines process development, synthesis, purification, analytical testing, and scale-up under a controlled quality framework.
Our capabilities support both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) production, from milligram to gram-scale quantities, including complex and modified DNA constructs for research, diagnostic, and commercial applications.
Through extensive collaboration with customers, our workflows have been optimized to ensure scalability, reproducibility, and consistent quality across projects ranging from early-stage development to large-scale manufacturing.
Each project follows a structured workflow designed to transition efficiently from concept to production:
DNA oligonucleotides can be engineered at three structural levels: the nucleobase, the deoxyribose sugar, and the internucleotide backbone linkage. This architecture-based approach allows DNA to be optimized for hybridization, stability, nuclease resistance, labeling, conjugation, and biological function.
Modified DNA bases and nucleobase analogs can tune recognition, labeling, hybridization, and functional behavior.
Modified sugar systems can improve duplex behavior, structural rigidity, and nuclease resistance.
Modified linkages can alter stability, charge, degradation profile, and biological performance.
Bio-Synthesis supports large-scale DNA production through optimized synthesis, purification, and scale-up workflows. Our platform enables both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) manufacturing, including modified DNA constructs for research, diagnostic, and commercial applications.
The pricing below applies to standard unmodified DNA oligonucleotides up to 100 bases. For longer sequences (up to 200 bases/base pairs), duplex DNA, or modified DNA, please contact us or request a custom quote.
Large-Scale Single-Stranded DNA (ssDNA)
Price for sequences up to 100 bases. For DNA oligos >100 bases, contact us.
Large-Scale Duplex DNA (dsDNA)
Price for sequences up to 100 bases. For duplex DNA >100 bases, contact us.
Bio-Synthesis can incorporate DNA-focused modifications into large-scale oligonucleotide projects, including position-specific modifications across nucleobases, sugars, backbone linkages, termini, and internal sites.
DNA base modifications and nucleobase analogs for altered recognition, labeling, epigenetic studies, and hybridization tuning.
DNA-compatible sugar modifications and analog systems to tune structure, duplex stability, and enzymatic resistance.
Internucleotide linkage modifications can improve stability, nuclease resistance, and biological performance.
5-prime and 3-prime terminal modifications for labeling, immobilization, conjugation, blocking, and downstream attachment.
Internal modifications can be placed within the sequence for probe design, structural spacing, or functional presentation.
DNA can be prepared with functional groups for coupling to peptides, proteins, antibodies, polymers, nanoparticles, surfaces, or small molecules.
Bio-Synthesis supports scalable DNA manufacturing from feasibility and pilot batches through larger production requirements. Large-scale DNA projects can be developed around sequence length, chemistry, target quantity, purification needs, and final application.
Production can be planned from development-scale quantities to larger research, diagnostic, or therapeutic-development lots.
Support for long, modified, duplex, and conjugation-ready DNA constructs where standard catalog workflows are not sufficient.
Projects can be optimized before scale-up to reduce synthesis, purification, and recovery risks.
Large-scale DNA quality depends on both synthesis and downstream purification. Bio-Synthesis can pair large-scale DNA synthesis with purification, analytical testing, release QC, and documentation packages appropriate for research, diagnostic, GLP-aligned, or GMP-supportive workflows.
Purification is selected based on sequence length, modification type, impurity profile, scale, and required final purity.
QC panels can verify identity, purity, impurities, residuals, general attributes, and safety-related parameters.
Documentation can be scaled from basic analytical reports to audit-ready release packages.
Custom DNA projects can benefit from early feasibility review, pilot synthesis, purification optimization, and analytical-method planning before committing to larger-scale manufacturing.
Quality support can be aligned with the intended use of the DNA material, from RUO to diagnostic or therapeutic-development programs.
Following successful development and pilot-scale production, Bio-Synthesis supports the transition of DNA oligonucleotide projects into larger-scale and regulated manufacturing workflows.
Our team works closely with customers to ensure that synthesis, purification, and analytical methods are reproducible and transferable from research-scale to production-scale environments. Our goal is to be your long-term manufacturing partner—delivering high-purity DNA oligonucleotides at every stage of your program, with the quality, reliability, and documentation your success depends on.
Large-scale ssDNA or dsDNA formats for donor templates, repair templates, and CRISPR-related workflows.
High-purity DNA probes with optional labels, quenchers, spacers, and affinity tags for assay development.
Custom DNA sequences for assembly workflows, functional testing, and engineered biology applications.
ssDNA and dsDNA for binding studies, annealing experiments, capture assays, and sequence-specific detection.
Modified DNA oligonucleotides for antisense, stability, nuclease resistance, targeting, and delivery research.
DNA with reactive handles for attachment to peptides, proteins, antibodies, polymers, nanoparticles, and surfaces.
Large-scale DNA synthesis is part of Bio-Synthesis’ broader oligonucleotide, analytical, process-development, and bioconjugation service platform.
For the fastest review, send the DNA sequence, requested format, length, quantity, desired modifications, purification requirement, QC/documentation requirements, and intended application.
Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.