Gene and DNA Synthesis Services

Custom gene and DNA synthesis services for research and development, including gene fragments, full-length genes, codon optimization, cloning, plasmid preparation, and sequence verification. Flexible support from simple DNA constructs to complex multi-step gene build projects.

Gene synthesis DNA fragments full-length genes codon optimization cloning and plasmids sequence verification research and development

Custom Protein Expression Services Overview

Gene and DNA synthesis provides a reliable route for building custom DNA constructs without relying on traditional cloning alone. Our platform supports both routine and complex synthesis workflows, helping researchers move from sequence design to functional construct generation with greater speed and flexibility.

We support projects ranging from short DNA fragments and full-length genes to optimized coding sequences, vector-ready cloned constructs, and plasmid DNA deliverables for protein expression, assay development, gene editing, RNA production, and synthetic biology workflows.

Typical length ranges: Gene fragment synthesis commonly covers approximately 100 to 1,500 bp, while full gene synthesis more commonly spans about 500 bp to 10,000+ bp, depending on sequence complexity, assembly strategy, and final deliverable format.

Gene and DNA synthesis workflow from project review through delivery
Flexible workflow: Projects can begin from a target gene sequence, an optimization request, a vector-ready insert design, or a defined plasmid construction plan.

Construct type

Fragments to genes

Short DNA fragments through full-length genes

Workflow

Design to plasmid

Sequence design, synthesis, cloning, and preparation

Optimization

Codon + sequence

Application-fit design and optimization support

Delivery

DNA or plasmid

Multiple output formats depending on program needs

Core Capabilities

Custom Gene Synthesis

Full-length gene synthesis for research, development, and downstream expression or functional studies.

DNA Fragment Synthesis

Custom DNA fragments for assembly workflows, cloning strategies, and assay development.

Codon Optimization

Sequence optimization aligned to host system, protein expression goals, and design constraints.

Cloning and Subcloning

Insertion into standard or project-specific vectors for downstream use.

Plasmid Preparation

Plasmid DNA preparation for sequencing, expression, transcription, or transfection workflows.

Sequence Verification

Construct confirmation and documentation to support project confidence and reproducibility.

Design Options

Sequence Engineering

  • Codon optimization
  • Restriction site removal or insertion
  • GC balancing where appropriate
  • Tag or linker incorporation

Construct Planning

  • Vector-ready insert design
  • Promoter or regulatory element inclusion
  • Mutation or variant incorporation
  • Application-specific build strategies

Gene Fragment vs Full Gene Synthesis: Key Differences

Understanding the difference between gene fragment synthesis and full gene synthesis helps determine the most efficient and cost-effective approach for your project.

Feature Gene Fragment Synthesis Full Gene Synthesis
Typical Length 100 – 1,500 bp (up to ~2 kb possible) 500 bp – 10,000+ bp (larger with assembly)
Format Linear double-stranded DNA Cloned into plasmid vector
Delivery Form Lyophilized DNA or solution Plasmid DNA, glycerol stock, or purified DNA
Turnaround Time Generally faster Longer due to cloning and verification
Cost Lower cost Higher cost due to cloning and QC steps
Typical Use PCR, Gibson assembly, cloning, mutagenesis, and synthetic biology workflows Protein expression, mRNA transcription, stable constructs, and functional studies
Cloning Required Yes, in most downstream workflows No, already cloned into vector
Sequence Verification Optional or limited depending on workflow Full sequence verification typically included
Complexity Handling Moderate; repetitive or GC-rich sequences may require added planning Better suited for longer and more complex constructs
Best For Flexible assembly and rapid prototyping Ready-to-use constructs for downstream applications
Practical insight: Large gene constructs are often built from multiple synthesized DNA fragments followed by assembly and cloning into plasmid vectors.

Typical Deliverables

Deliverable Type Description Typical Use
Linear DNA Fragment Synthesized DNA fragment without vector cloning Assembly, PCR, cloning workflows
Cloned Gene Gene inserted into a cloning or expression vector Expression, transcription, plasmid-based studies
Sequence-Verified Plasmid Verified plasmid construct with documentation Downstream development and reproducible use

Final deliverable format depends on construct design, cloning scope, and downstream application requirements.

Applications

Protein Expression

Gene constructs designed for recombinant protein production and expression screening.

mRNA and RNA Workflows

DNA templates and plasmids prepared for IVT RNA and mRNA production projects.

Gene Editing and Functional Studies

Custom constructs for CRISPR tools, reporter systems, and synthetic biology applications.

FAQ

Do you provide both gene fragments and full-length genes?

Yes. We support projects ranging from short DNA fragments to full-length gene synthesis depending on construct requirements.

Can you optimize the sequence before synthesis?

Yes. Codon optimization and sequence engineering can be included when aligned to expression goals, host system, and downstream application.

Can the synthesized gene be cloned into a plasmid?

Yes. We can provide vector-ready cloned constructs and plasmid preparation depending on the selected workflow.

How are constructs verified?

Construct verification can include sequence confirmation and project-fit documentation before final delivery.

What is the difference between a gene fragment and a full gene?

A gene fragment is typically delivered as linear double-stranded DNA and is commonly used for assembly or cloning workflows. A full gene is more often cloned into a plasmid vector, sequence-verified, and ready for downstream expression, transcription, or functional studies.

Contact & Quote Request

For the fastest quote, include target sequence information, construct length, whether codon optimization is needed, cloning requirements, preferred vector or delivery format, and any downstream application details.

What to include

  • Target sequence or construct plan
  • Desired length and format
  • Optimization or mutation requirements
  • Cloning, plasmid, or downstream workflow needs

Fastest path

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.