Large-Scale RNA Synthesis

Large-scale RNA synthesis is one of Bio-Synthesis’ highest-demand oligonucleotide manufacturing capabilities, supporting increasingly complex RNA programs in therapeutics, diagnostics, gene regulation, gene editing, delivery, and advanced molecular assay development.

Our platform supports standard and highly modified RNA oligonucleotides, including single-stranded RNA, duplex RNA, siRNA, antisense RNA, RNA aptamers, guide RNA components, labeled RNA probes, and conjugation-ready RNA constructs.

Bio-Synthesis has refined RNA synthesis, deprotection, purification, analytical testing, and scale-up workflows to support high-purity RNA manufacturing with reproducible quality and documentation.

From RNA Design to Large-Scale Production

From RNA Design to Large-Scale Production

• Design & Feasibility: sequence evaluation, modification strategy, duplex design, and scale planning
• Synthesis: optimized RNA phosphoramidite chemistry and coupling strategy
• Deprotection & Handling: RNA-sensitive cleavage, deprotection, and recovery workflows
• Purification: HPLC, PAGE, IEX, and application-specific purification options
• Quality Control: LC-MS, HPLC, impurity profiling, duplex assessment, and release testing
• Documentation & Scale-Up: CoA, batch records, traceability, and transition to larger production

RNA oligonucleotides can be engineered at three structural levels: the nucleobase, the ribose sugar, and the internucleotide backbone linkage. Bio-Synthesis provides extensive RNA modification capabilities across all three dimensions to improve stability, activity, binding, nuclease resistance, delivery, and analytical performance.

RNA can be engineered at the base, sugar, and backbone level to support stability, activity, recognition, and delivery requirements.

RNA Base Engineering

Modified RNA bases can tune immune recognition, pairing behavior, stability, binding, labeling, and biological performance.

RNA Sugar Engineering

2′ sugar modifications are central to RNA stability, nuclease resistance, duplex properties, and therapeutic design.

RNA Backbone Engineering

Backbone linkage modifications can improve nuclease resistance, pharmacology, and compatibility with therapeutic or diagnostic workflows.

Bio-Synthesis supports multiple RNA formats at development and large-scale quantities. Projects can be manufactured as standard RNA, heavily modified RNA, duplex RNA, labeled RNA, or conjugation-ready RNA.

Bio-Synthesis supports large-scale RNA synthesis from 50 mg to multi-gram quantities, including standard and highly modified RNA oligonucleotides. Our platform is optimized for duplex RNA, siRNA, guide RNA components, labeled RNA probes, and complex modified RNA constructs.

Manufacturing scale, yield, and purity are influenced by sequence length, modification type, duplex design, and purification requirements. Each RNA project is evaluated to support optimal synthesis efficiency, recovery, and reproducibility at scale.

The pricing below applies to standard RNA oligonucleotides under defined conditions. For modified RNA, duplex RNA, guide RNA, conjugation-ready RNA, or sequences requiring specialized purification and analytical testing, please contact us or request a custom quote.

Optional services such as advanced purification, analytical testing, release QC, CoA documentation, process development, and scale-up support can be incorporated based on project needs. Learn more in our Purification, Quality Control, and Documentation section.

Transparent pricing for standard RNA. Custom pricing available for advanced RNA designs.

Large-scale RNA projects are processed in an RNase-free environment with configurable purification, QC, formulation, packaging, and documentation options based on the intended application.

Example Pricing: HPLC-Purified Unmodified RNA, 10–30-mer, PO Backbone

Final Amount	Price (USD)
50 mg	$3,200
100 mg	$4,800
250 mg	$6,500
500 mg	$8,000
1,000 mg	$10,500
>1,000 mg	Inquire

Representative large-scale RNA lots. Packaging varies by order and project requirements.

Prices are examples for unmodified RNA 10–30 nt and include HPLC purification and diafiltration. Final cost varies with sequence composition, modification pattern, purification requirements, QC testing, formulation, and documentation needs.

Extensive RNA modification capabilities across base, sugar, and backbone chemistry

Bio-Synthesis provides extensive RNA modification capabilities, including base modifications, 2′ sugar modifications, backbone modifications, terminal labels, internal labels, spacers, and conjugation-ready functional handles. These modifications are commonly used to improve RNA stability, nuclease resistance, potency, biodistribution, targeting, and assay performance.

Engineered for complex RNA synthesis, scale-up performance, and reproducible manufacturing outcomes.

Large-scale RNA manufacturing requires tight control over synthesis, deprotection, purification, analytical testing, and storage conditions. Bio-Synthesis supports RNA projects from feasibility and pilot production through larger manufacturing requirements.

Large-scale RNA quality depends on synthesis efficiency, RNA-sensitive deprotection, nuclease-safe handling, purification, analytical testing, and release documentation.

Following successful RNA development and pilot-scale production, Bio-Synthesis supports the transition of RNA oligonucleotide projects into larger-scale and regulated manufacturing workflows.

Our team works closely with customers to ensure that RNA synthesis, purification, duplex handling, and analytical methods are reproducible and transferable from research-scale to production-scale environments. Our goal is to be your long-term RNA manufacturing partner—delivering high-purity RNA oligonucleotides at every stage of your program, with the quality, reliability, and documentation your success depends on.

• Process transfer: transition of synthesis, deprotection, purification, and annealing workflows to larger-scale production
• Scale-up optimization: adjustment of reaction conditions, purification, recovery, and formulation
• Analytical method transfer: consistency in QC testing, impurity tracking, and release criteria
• Documentation support: batch records, method descriptions, traceability, and CoA documentation
• cGMP readiness: support for RNA programs requiring higher levels of quality control and regulatory alignment

Built for RNA programs that require chemistry depth, scale, quality, and documentation.

Large-scale RNA synthesis is part of Bio-Synthesis’ broader oligonucleotide, analytical, process-development, and bioconjugation service platform.

For the fastest response, share your RNA sequence, scale, and modification requirements with our scientific team.