Long RNA in vitro transcription is often the preferred route when RNA constructs extend beyond practical chemical synthesis lengths or when programs require a flexible template-driven workflow. We support both single-stranded RNA (ssRNA) and double-stranded RNA (dsRNA) production for applications ranging from functional genomics and RNA biology to RNA interference workflows.
Our service is structured around two practical entry points. The first is a full-service workflow in which we support the program from gene synthesis and template construction through RNA transcription, optional modification, and conjugation. The second is a transcription-only workflow in which clients provide the DNA template and we perform transcription, purification, and QC.
A major differentiator is scale flexibility. We support projects from microgram quantities for early feasibility and screening through gram-scale production for larger development needs, allowing one service framework to cover both small pilot work and expanded program requirements.
For customers who want a clearer view of how our platform works, the manufacturing process can be summarized in five phases: Phase I, synthesizing the gene of the target RNA; Phase II, plasmid DNA expansion; Phase III, plasmid preparation for RNA synthesis; Phase IV, RNA synthesis by in vitro transcription; and Phase V, purification and storage of the properly sized product.
Illustrative phase-based workflow for long RNA manufacturing, from gene synthesis through plasmid expansion, IVT RNA synthesis, purification, and storage.