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mRNA Transcription Services

ISO 9001:2015 and ISO 13485:2016 certified mRNA transcription services supporting both enzymatic IVT mRNA production and chemical capped RNA synthesis, with scalable workflows from microgram to gram quantities and support for very long mRNA constructs over 10,000 bases. Choose either a full-service workflow from gene synthesis through mRNA production, modification, purification, and downstream support, or mRNA production from your supplied DNA template.

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Overview Service Models Production Approaches mRNA Features Modifications Packages Expected Yield Analytical Services Contact

Custom mRNA Synthesis Services Overview

mRNA transcription services require more than simply generating a transcript. Successful programs often depend on the right combination of transcript design, capping strategy, poly(A) tailing, modified nucleotide selection, purification, and quality control. Our mRNA platform is built to support both discovery-stage and advanced development workflows with flexible technical pathways matched to project needs. A key strength of our platform is support for very long mRNA transcripts over 10,000 bases, helping programs that require extended open reading frames, large constructs, or specialized long-transcript designs.

Our platform supports custom mRNA synthesis services, including in vitro transcription (IVT) mRNA production, capped mRNA synthesis, and scalable mRNA manufacturing services for research and advanced development.

IVT mRNA synthesis 5' capping poly(A) tailing modified nucleotides ISO 9001:2015 / ISO 13485:2016 U.S. Facilities in Texas Bench to Gram-scale Production >10,000-base mRNA capability

We provide two practical service models. The first is a full-service workflow that starts with gene synthesis or template construction and continues through mRNA production, modification, purification, and project-aligned QC. The second is a client-template workflow for teams that already have a validated plasmid DNA or PCR template and need efficient mRNA production and downstream processing.

A key differentiator is our ability to support both enzymatic IVT mRNA production and chemical capped RNA synthesis. This gives programs more flexibility when choosing the best method based on transcript length, capping requirements, modification strategy, scale, and downstream application. For longer constructs, see our long RNA IVT transcription services.

mRNA transcription workflow including IVT synthesis, capping, purification, and formulation
Flexible process: The workflow can start from sequence concept, customer-supplied DNA template, or a defined chemical capped RNA design depending on program needs.

Production scale

microgram to gram

Supports early screening through larger development needs

Workflow model

2 pathways

Full service or client-supplied DNA template

mRNA engineering

Cap + tail

Capping, poly(A), and modification support

Quality systems

Dual ISO

ISO 9001:2015 and ISO 13485:2016 certified

Custom mRNA Transcription Services: Full-Service and Client-Supplied Template Options

1. Full-Service mRNA Workflow

This option is designed for customers who want a single partner from sequence concept to final mRNA output.

  • Gene synthesis and template design support
  • Template generation, cloning, and plasmid preparation
  • mRNA production by IVT or chemical approach as appropriate
  • Capping, poly(A) tailing, and modification support
  • Purification, QC, and delivery

2. mRNA Production from Client-Supplied Template

This option is ideal when a validated plasmid DNA or PCR template is already available and the goal is rapid mRNA production with fit-for-purpose processing.

  • Production from client-provided plasmid DNA or PCR template
  • Flexible support for capped or uncapped transcript workflows
  • Poly(A) tailing and modification support as required
  • Purification and project-aligned QC
  • Faster path for established programs

IVT mRNA Synthesis vs Chemical Capped RNA: Choosing the Right Approach

Enzymatic IVT mRNA

In vitro transcription is the preferred approach for longer mRNA constructs and scalable production.

  • Well suited for long mRNA constructs, including transcripts over 10,000 bases
  • Supports scalable production from microgram to gram quantities
  • Compatible with capping, poly(A) tailing, and modified nucleotides
  • Useful for protein expression, vaccines, CRISPR delivery, and broader research applications

Chemical Capped RNA Synthesis

Chemical RNA synthesis provides precise sequence control and can support capped RNA constructs in appropriate project settings.

  • High sequence precision and defined structural control
  • Supports selected capping and modification strategies
  • Useful for shorter or highly defined RNA designs
  • Helpful when exact structural control is more important than long transcript length
Unique capability: Unlike many providers, we support both enzymatic IVT mRNA production and chemical capped RNA synthesis within the same platform, enabling flexible design strategies across a wide range of transcript lengths and applications.

mRNA Design Features: Capping, Poly(A) Tailing, and Modified Nucleotides

Capping Options

  • Cap 0
  • Cap 1
  • Co-transcriptional capping
  • Post-transcriptional capping

Poly(A) Tailing

  • Encoded poly(A) designs
  • Post-transcriptional tailing options
  • Tail-length planning support
  • Application-fit design flexibility

Modified Nucleotides

  • N1-methyl-pseudouridine
  • Pseudouridine
  • 5-methylcytidine
  • Project-specific modification strategies

Scalable Output

  • Microgram screening quantities
  • Milligram development scale
  • Larger production campaigns
  • Flexible workflow planning

Common Base & Nucleoside Modifications

We support a range of base, nucleoside, and structural modifications to optimize mRNA stability, translation performance, and application fit.

Modified Bases

  • Pseudouridine (Ψ)
  • N1-methyl-pseudouridine (m1Ψ)
  • 5-methylcytidine (m5C)
  • 5-methyluridine (m5U)
  • 2-thiouridine (s2U)

mRNA Structural Enhancements

  • Cap 0 / Cap 1 structures
  • Custom 5′ end strategies
  • Custom 3′ end strategies
  • Poly(A) tailing options

Labeling & Conjugation

  • Biotin labeling
  • Fluorescent labeling
  • Click-compatible handles
  • Custom conjugation support
Flexible integration: Modifications can be incorporated during synthesis or introduced post-production depending on transcript design, workflow, and downstream application requirements.

mRNA Manufacturing Service Packages

Package selection depends on your required level of purity, contamination control, and analytical validation. Tiered options support different mRNA quality expectations and analytical depth.

Standard Package

  • Routine QC and transcript verification
  • Fit-for-purpose production for general research
  • Efficient path for early screening

Silver Package

  • Higher stringency purity review
  • Enhanced contamination control
  • Useful for more sensitive workflows

Gold Package

  • Independent lot-to-lot processing emphasis
  • High stringency contamination control
  • Structured QC for advanced projects

qPCR-based analysis is not included unless the client provides assay primers or sufficient sequence information.

Gold Package Plus

  • Includes all Gold features
  • qPCR-based analysis when assay information is available
  • Higher confidence in analytical evaluation

Requires client-provided assay primers or sufficient sequence information to design the qPCR assay.

Expected mRNA Yield by Transcription Scale

Expected mRNA yield depends on transcription scale, sequence length, nucleotide composition, and whether additional processing such as capping, poly(A) tailing, or modified nucleotide incorporation is required.

Transcription Scale RNA Expected Yield
(uncapped, no poly(A) tail)		 mRNA Expected Yield
(capped, poly(A) tailing)		 Plasmid DNA Requirement
0.1 mL 0.02 – 0.3 mg 0.01 – 0.1 mg ~15 µg
0.5 mL 0.5 – 1.5 mg 0.25 – 0.75 mg ~50 µg
1.0 mL 1 – 3 mg 0.5 – 1.5 mg ~100 µg
2.0 mL 2 – 6 mg 1 – 3 mg ~200 µg
4.0 mL 4 – 12 mg 2 – 6 mg ~400 µg
6.0 mL 6 – 18 mg 3 – 9 mg ~600 µg
10 mL 10 – 30 mg 5 – 15 mg ~1000 µg
Important note: Final mRNA yield is typically lower than uncapped RNA due to additional processing steps such as capping, poly(A) tailing, purification, and modification incorporation.

Actual yields vary depending on sequence complexity, transcript length, GC content, and modification strategy, with very long constructs including transcripts over 10,000 bases typically requiring project-specific planning. These values are provided as planning ranges for project design and quoting.

Analytical & Purification Services

Purification Options

  • Project-fit purification workflows
  • Chromatography-based strategies as appropriate
  • Polishing for downstream use
  • Approaches selected by transcript and application

Transcript Characterization

  • Identity and concentration verification
  • Purity and size assessment
  • 5′ capping review where applicable
  • Poly(A) tailing evaluation where applicable

Residual & Impurity Testing

  • Residual DNA / template review
  • Contamination-control strategies
  • qPCR-based analysis in supported package tiers
  • Project-fit analytical planning for sensitive workflows
Analytical depth matters: One major differentiator in mRNA services is not only making transcript, but demonstrating fit-for-purpose purity, capping, tailing, and residual control for the intended application.

Template Requirements & Ordering Guidance

Client-Supplied Template Requirements

  • T7 promoter preferred for IVT-based workflows
  • Correctly positioned target sequence downstream of the promoter
  • Suitable plasmid DNA or PCR template quality
  • Sequence and design review recommended before production

Project Planning Details

  • Lead time varies by design complexity, scale, and analytical scope
  • Minimum practical quantity depends on workflow and transcript design
  • Capping, tailing, and modification choices affect schedule and yield
  • Quote review is recommended for complex or highly modified projects
For fastest quoting: Provide sequence or template details, intended scale, capping requirement, poly(A) plan, modification strategy, analytical needs, and downstream application.

Regulatory and Quality Standards for mRNA Manufacturing Services

ISO-Certified Operations

  • ISO 9001:2015 certified quality management system
  • ISO 13485:2016 certified processes
  • Controlled production workflows
  • Project-fit documentation and traceability practices

Program Fit

  • Research and preclinical workflows
  • Assay development and validation
  • Structured quality systems for advanced programs
  • Flexible support across multiple mRNA development stages

Applications for Custom IVT mRNA and Capped mRNA Synthesis

Protein Expression

  • Transient protein expression workflows
  • Screening and functional expression studies
  • Cell-based research programs

Therapeutic & Vaccine R&D

  • mRNA vaccine research
  • Gene and protein replacement concepts
  • Advanced formulation and delivery studies

Genome Editing & Tools

  • Cas mRNA production
  • Editor-related workflows
  • Research-stage CRISPR delivery studies

Technical Resources

Explore technical guidance for mRNA design, template submission, and analytical planning to help define the right workflow, quality scope, and production strategy for your project.

mRNA Design Guide

Guidance on transcript design, capping strategy, poly(A) tailing, modified nucleotides, and selecting IVT versus chemical capped RNA workflows.

Template Submission Guide

Requirements for plasmid DNA and PCR templates, promoter placement, sequence review, submission expectations, and project-start planning.

Analytical Options Overview

Overview of purity assessment, qPCR-supported analysis, capping and tailing review, residual impurity evaluation, and package-level QC options.

Need project guidance? These resources are designed to help you define the right mRNA workflow before requesting a quote.

FAQ

Do you offer full-service mRNA production?

Yes. We support full-service workflows from gene synthesis and template generation through mRNA production, capping, poly(A) tailing, purification, and project-aligned QC.

Can you work from client-supplied plasmid DNA or PCR templates?

Yes. We support mRNA production from client-provided plasmid DNA or PCR templates when the template design is appropriate for the selected workflow.

Do you support both IVT mRNA and chemical capped RNA synthesis?

Yes. We support both approaches and help determine the most suitable production route based on transcript length, capping requirements, modification strategy, structural control, and scale.

Can modified nucleotides be incorporated?

Yes. Common modified nucleotides such as pseudouridine and N1-methyl-pseudouridine can be incorporated when the project requires improved stability, reduced immunogenicity, or performance optimization.

What quality systems do you operate under?

Our operations include ISO 9001:2015 and ISO 13485:2016 certified quality systems, supporting structured workflows and program-aligned documentation.

Which capping approaches do you support?

Support can include Cap 0, Cap 1, co-transcriptional capping, and post-transcriptional capping strategies depending on project needs.

Can you support both research and advanced development programs?

Yes. We support workflows from early research quantities through larger development-scale programs, with flexible production and QC planning.

Can you produce very long mRNA constructs over 10,000 bases?

Yes. One of our platform strengths is support for very long mRNA constructs over 10,000 bases. Because very long transcripts require careful template design, transcription planning, and purification strategy, we recommend sequence review during quoting.

What information do you need for a quote?

Please share whether you need a full-service or client-template workflow, sequence or template details, target quantity, capping requirements, poly(A) tailing needs, any modified nucleotide strategy, analytical requirements, and timeline expectations.

More FAQ'sAll FAQ's

Contact & Quote Request

For the fastest quote, include whether you need a full-service workflow or production from a client-provided template, along with sequence or template details, target quantity, capping requirement, poly(A) tailing needs, any modified nucleotide strategy, and downstream goals.

What to include

  • Full-service or client-template workflow
  • Sequence or DNA template details
  • Target quantity from microgram to gram scale
  • Capping, tailing, modification, or labeling requirements

Fastest path

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