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Custom Long RNA Synthesis up to 275 nt

Chemical synthesis and IVT-based RNA production for research, CRISPR workflows, synthetic biology, mRNA development, and modified RNA applications.

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Overview Capabilities Specifications Applications FAQ Contact

Overview

Long RNA molecules are increasingly important in therapeutic research, assay development, CRISPR workflows, structural biology, and synthetic biology. Our custom long RNA chemical synthesis service is designed to provide researchers with precise RNA sequences, along with the control, customization, and consistency required for complex research and development programs.

Bio-Synthesis, a U.S.-based company, offers chemical long RNA synthesis up to 275 nt for ssRNA or dsRNA as well as in vitro transcription (IVT)-based RNA production for generating significantly longer RNA transcripts from DNA templates. These ready-to-use RNA products include custom mRNA, tRNA, and long noncoding RNA (lncRNA), with support for a wide range of modifications and labeling strategies.

Long RNA is produced using solid-phase chemical synthesis for precise sequence-defined constructs up to 275 nt, with the option to transition to in vitro transcription (IVT) for significantly longer RNA sequences. We support a range of RNA modifications including base, sugar, and backbone modifications, as well as labeling strategies to support structure-function studies, stability optimization, and assay development.

Long RNA synthesis workflow showing RNA synthesizer followed by purification and PAGE analysis

Long RNA synthesis workflow. Solid-phase chemical synthesis followed by purification and analytical characterization including PAGE and instrument-based analysis.

Service Highlights

275 nt

Chemical synthesis

Up to 5 mg

Available yield

IVT

Extended RNA capability

Custom

Sequence-by-sequence design

Modified RNA

Base, sugar & backbone options

Our long RNA synthesis workflow is optimized for performance and reliability. Each RNA oligonucleotide undergoes rigorous quality control using techniques such as PAGE, HPLC, and/or mass spectrometry. Products are packaged using our proprietary RNaseSAFE™ system in RNase- and DNase-free tubes to protect against degradation during shipping and storage and to preserve biological activity.

Complexity

Built for sequence complexity

Support challenging long RNA constructs with careful attention to sequence composition, length, and synthesis strategy.

Customization

Customization-focused

Configure RNA synthesis based on sequence length, scale, modification strategy, purification, and analytical requirements.

Application

Application-driven

Designed for research teams developing RNA tools, assay reagents, and next-generation therapeutic concepts.

Service Capabilities

RNA Custom

Custom sequence submission

Submit single long RNA targets or broader sequence panels for research-driven development workflows.

Use case: exploratory and project-based ordering

Modified Flexible

Selectable modification options

Modification strategies can be aligned with sequence feasibility and intended use.

Use case: advanced RNA research constructs

QC Purification

Analytical and purification support

Purification and QC packages can be matched to the complexity and goals of each project.

Use case: fit-for-purpose research material

What we support

Custom RNA sequences up to 275 nt • project-specific design review • selected modification support • application-aligned purification • analytical characterization • technical consultation during quoting and planning.

Extended RNA capability: For applications requiring longer RNA beyond chemical synthesis limits, we also provide in vitro transcription (IVT)-based RNA production for extremely long RNA constructs.

Service Specifications

Our long RNA synthesis service supports extended sequence lengths and higher yield requirements compared to standard guide RNA offerings. Specifications can be adjusted based on sequence design and project requirements.

Category Specification
RNA Type Custom long RNA (mRNA, tRNA, lncRNA, guide RNA-related constructs)
Length Up to 275 nt (chemical synthesis); longer RNA available via IVT
Format Extended ssRNA and dsRNA constructs for research applications
Scale / Yield Up to milligram scale (up to ~5 mg depending on sequence and method)
Synthesis Method Solid-phase chemical synthesis; in vitro transcription (IVT) for extended length
Modification Options Base, sugar, and backbone modifications; labeling (e.g., fluorescent, biotin)
Purification Options Desalting, cartridge, HPLC, PAGE (based on sequence and application)
Quality Control PAGE, HPLC, and/or mass spectrometry (MS)
Packaging RNaseSAFE™ packaging in RNase- and DNase-free tubes
Applications Therapeutic research, CRISPR workflows, assay development, synthetic biology

Key differentiation

Compared to standard guide RNA services, this offering supports longer RNA sequences and higher yield production, enabling broader applications in advanced research and development programs.

RNA Modifications for Functional Enhancement

Chemical modifications can be incorporated into long RNA sequences to improve stability, enhance performance, and enable specialized applications. Modification strategies are selected based on sequence design, synthesis feasibility, and intended use.

Base

Base modifications

Modify nucleobases to support structure-function studies, improve recognition, or enhance assay performance.

Sugar

Sugar modifications

Incorporate modifications such as 2′-O-methyl or 2′-fluoro to improve nuclease resistance and stability.

Backbone

Backbone modifications

Modify the phosphate backbone to enhance durability and tune biological interactions.

Labeling

Labeling

Add fluorescent dyes, biotin, or other labels for detection, tracking, and assay development applications.

Bioconjugation

Bioconjugation

Conjugate RNA to peptides, lipids, or other molecules to enable delivery, targeting, or functional enhancement.

Custom

Custom modification strategies

Combine multiple modification types within a single RNA construct to meet specific research or application needs.

Design considerations

Modification selection depends on sequence length, synthesis compatibility, structural impact, and downstream application requirements. Our team can assist in selecting appropriate strategies for each project.

Methods for Long RNA Synthesis

Long RNA constructs can be generated using different synthesis approaches depending on sequence length, design complexity, and application requirements.

Chemical

Solid-phase chemical synthesis

Enables precise, sequence-defined RNA production up to 275 nt with support for site-specific modifications and controlled synthesis.

IVT

In vitro transcription (IVT)

Suitable for generating longer RNA constructs beyond chemical synthesis limits using DNA templates and enzymatic transcription.

Hybrid

Hybrid strategies

Combine chemical synthesis and enzymatic methods to balance sequence precision, modification placement, and overall length.

Method selection

Selection of synthesis method depends on RNA length, required modifications, yield targets, and application. Chemical synthesis offers precision and modification flexibility, while IVT enables access to longer RNA sequences.

Applications

Common long RNA application areas

  • mRNA and circular RNA research support
  • CRISPR and guide RNA-related development
  • RNA structure-function studies
  • Diagnostic and assay reagent development
  • Modified RNA evaluation programs
  • Synthetic biology and discovery workflows

Why researchers use this service

  • Access longer custom RNA constructs with defined specifications
  • Support site-specific design needs and selected chemical modifications
  • Align purification and QC options with experimental goals
  • Reduce friction between concept development and material supply

Project support model

Our team can review target length, sequence complexity, modification requirements, purification preferences, and intended research application to recommend a suitable long RNA synthesis path.

Extended capability: For sequences exceeding chemical synthesis limits, IVT-based RNA production can be recommended to access significantly longer RNA constructs.

FAQ

What RNA lengths are supported?

We support custom long RNA chemical synthesis up to 275 nt, depending on sequence design and project requirements.

Can modified RNA be requested?

Yes. Modification options can be discussed during project review and are assessed based on sequence feasibility and intended application.

What information should I provide for a quote?

Please provide the RNA sequence, target length, scale, modification requirements, purification preferences, and any relevant project notes.

Is this suitable for therapeutic development work?

This page is currently written for research and development use. Final suitability depends on your program stage and required quality framework.

More FAQ'sAll FAQ's

Talk to a Scientist

Share your target sequence length, modification needs, purification requirements, and intended application. We can help shape the page language around your actual service workflow and quoting process.

Sequence review Modification planning Purification options Analytical support

What to include

  • RNA sequence and length
  • Requested scale
  • Desired modification(s)
  • Purification preference
  • Intended research application
  • Target timeline
Request a Quote Contact

Confidential technical consultation • Rapid feasibility review • Research support

Recommended Reading & Literature References

Selected references related to long RNA synthesis, RNA modifications, and applications in therapeutics, CRISPR workflows, and synthetic biology.

  1. Sahin, U.; Karikó, K.; Türeci, Ö. mRNA-based therapeutics — developing a new class of drugs. Nat. Rev. Drug Discov. 2014, 13, 759–780. DOI
  2. Karikó, K.; Buckstein, M.; Ni, H.; Weissman, D. Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification. Immunity 2005, 23, 165–175. DOI
  3. Hendel, A.; et al. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat. Biotechnol. 2015, 33, 985–989. DOI
  4. Milligan, J. F.; Groebe, D. R.; Witherell, G. W.; Uhlenbeck, O. C. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 1987, 15, 8783–8798. DOI
  5. Motorin, Y.; Helm, M. RNA nucleotide methylation. Wiley Interdiscip. Rev. RNA 2011, 2, 611–631. DOI
  6. Nandagopal, N.; Elowitz, M. B. Synthetic biology: integrated gene circuits. Science 2011, 333, 1244–1248. DOI

Note: These references are provided for scientific context on long RNA synthesis, modification strategies, and applications. They are not intended to represent clinical claims.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

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