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Delivery & functional conjugates • discovery → multi‑kg scale

Oligonucleotide–Peptide Conjugation Platform

Site-specific peptide conjugation for siRNA, ASO, PNA, and PMO — supporting client-designed peptides or CPP options, with 3′/5′/internal placement and CMC-aligned analytics. Texas‑based CDMO manufacturing for CPP and custom peptide–oligonucleotide conjugates at gram‑to‑multi‑kg scale.

Talk to a Scientist Explore capabilities →
siRNA • ASO • PNA • PMO 3′ / 5′ / internal placement Client peptides or CPP Texas, USA manufacturing Gram → multi‑kg scale
Talk to a chemist Request a quote
Overview Capabilities Supported modalities Peptide options Attachment chemistry Scale & QC FAQ Recommended Reading

Overview

Peptide conjugation is a high-impact lever to improve cellular uptake, tissue targeting, intracellular trafficking, and functional modulation of oligonucleotide therapeutics. We provide a unified conjugation platform across siRNA duplexes, ASO single-strands, PNA, and PMO, enabling rapid screening in discovery and scalable supply for translation.

Conjugation can be engineered at the 3′ terminus, 5′ terminus, or internal positions (where design permits), including strand-specific placement for siRNA programs. Designs may use client-supplied peptides, in-house peptide synthesis, or established CPP motifs with customizable spacer and linker architecture.

CPP + targeting peptides Neutral backbone support (PNA/PMO)

Platform at a glance

Both oligonucleotides and peptides are produced in-house in Texas, USA for supply chain control and analytics continuity.

Modalities

siRNA / ASO / PNA / PMO

Placement

3′ / 5′ / internal

Peptides
Client or CPP options
Linkers
Stable or cleavable
Scale
Gram → multi‑kg
Location
Texas, USA
Why pharma teams use peptide conjugation

A platform view: delivery engineering + manufacturability + analytics.

Delivery Uptake
Improve cellular access

CPPs and engineered peptides can increase uptake and support intracellular trafficking designs."

Targeting Ligands
Enable tissue direction

Targeting peptides can bias distribution, supporting receptor-driven or tissue-homing approaches.

CMC Consistency
Scale with confidence

Conjugation strategy is selected to preserve function and reduce heterogeneity during scale-up.

Core Capabilities

In-house Texas
Integrated component manufacturing

Oligo and peptide production in Texas supports speed, traceability, and unified analytics.

Placement Flexible
Any-position conjugation

3′, 5′, or internal conjugation (design permitting), including strand-specific siRNA placement.

Scale CDMO
Discovery → multi‑kg supply

CMC-aligned purification and QC planning to support translation without redesign.

What makes this platform different

Many providers can conjugate a peptide to a single oligo type. We support siRNA, ASO, PNA, and PMO conjugates with any-position placement and integrated U.S. manufacturing for both components — designed for scale and analytical confirmation.

Supported Oligonucleotide Modalities

Peptide conjugation can be applied across multiple mechanisms (RNAi, RNase H, steric block, exon skipping) to improve delivery and functional performance.

Modality Format Typical therapeutic intent Why add a peptide
siRNA Duplex; strand-specific conjugation RNA interference (gene silencing) Improve uptake/trafficking; guide/passenger placement control
ASO Single-strand; gapmer or steric block RNase H or steric modulation Targeting peptides and uptake enhancement for intracellular delivery
PNA Neutral backbone; steric block Steric blocking / high-affinity binding Peptides can be especially impactful for uptake of neutral-backbone constructs
PMO Morpholino; steric block / exon skipping Splice modulation and exon skipping CPPs and targeting peptides can increase uptake for challenging tissues
Placement flexibility

We support conjugation at the 3′ terminus, 5′ terminus, or internal positions (design permitting) for each modality. For siRNA duplexes, specify strand selection and placement to align with your mechanism and design constraints.

Peptide Options (Client-Designed or CPP)

Client-designed peptides

Conjugate your proprietary peptide to siRNA, ASO, PNA, or PMO. We can evaluate client-supplied peptides (functional handle, purity, stability) or synthesize peptides in-house in Texas to support rapid iteration.

Linear / modified peptides Targeting ligands Cyclized/engineered (case-by-case)
Cell-Penetrating Peptides (CPP)

CPP conjugates can enhance uptake and trafficking. We support common CPP classes and can tune spacer/linker design to balance potency, handling, and batch homogeneity.

CPP class Examples Typical intent
Arginine-rich R8/R9 motifs, related polycationic sequences Uptake enhancement
Amphipathic Transportan/MAP-like motifs Trafficking / endosomal hypotheses
Penetratin-type Penetratin/TAT-like motifs Cell entry strategies
Custom engineered Client-defined sequences Program-specific design
Design considerations

Peptide charge density, hydrophobicity, and conjugation site influence uptake, purification behavior, and batch homogeneity. We can support early screens (lipid/peptide variants, spacer lengths, and attachment site) to identify a lead design suitable for scale.

Attachment Chemistry & Linker Architecture

Conjugation chemistry is selected to preserve both oligonucleotide activity and peptide functionality while enabling clear analytical confirmation. Stable and cleavable linker options are supported depending on your design intent.

Stable Robust
Stable linkers

Amide and thioether strategies for robust attachment and consistent storage/handling.

Cleavable Release
Cleavable linkers

Disulfide and other release strategies (design-dependent) to support intracellular release hypotheses.

Modular Click
Modular assembly

Azide/alkyne click and functional-handle strategies for modular peptide integration.

Position control

Specify 3′, 5′, or internal placement and (for siRNA) the intended strand. Spacer selection (TEG/PEG) can improve handling, reduce steric effects, and support consistent purification outcomes.

Scale, Purification & QC

Peptide conjugates introduce critical quality attributes: conjugation completeness, positional homogeneity, peptide integrity, and conjugate-specific impurities. Our workflows are designed to support discovery iteration and scale-up with CMC-aligned analytics.

Purification Resolution
Conjugate purification

Resolve unconjugated oligo, partially conjugated species, and positional variants when required.

Analytics Identity
Identity & purity confirmation

Orthogonal confirmation (e.g., LC‑MS + chromatographic profiles) aligned to stage and documentation needs.

Scale Control
Gram → multi‑kg manufacturing

Scale-aware processes with integrated component production in Texas for reliability and turnaround.

Request a technical discussion →

Talk to a Scientist

Tell us your modality (siRNA / ASO / PNA / PMO), sequence, peptide preference (client peptide or CPP), attachment position (3′/5′/internal), linker/spacer requirements, purity/QC targets, and quantity. If using a client-supplied peptide, include structure/sequence, functional handle, purity, and stability constraints.

Client peptide evaluation CPP options Any-position placement Texas-based manufacturing
What to include in your request
  • Modality (siRNA / ASO / PNA / PMO)
  • Sequence + key modifications
  • Peptide (client or CPP) + handle (Cys/amine/click)
  • Attachment site (3′/5′/internal) + spacer (TEG/PEG)
  • Purity/QC requirements and quantity target
Request a Quote Contact

FAQ

Which oligonucleotide types can you conjugate to peptides?

We support peptide conjugation for siRNA, ASO, PNA, and PMO modalities.

Can peptides be attached at any position?

We support 3′, 5′, and internal placement (design permitting), including strand-specific placement for siRNA duplexes.

Do you support client-designed peptides and CPP peptides?

Yes. We can evaluate client-supplied peptides or synthesize peptides in-house, and we support CPP options and custom designs.

Where are peptides and oligonucleotides manufactured?

Both components are produced in-house in Texas, USA, supporting supply chain control and integrated analytics.

Can you support large scale manufacturing?

Yes. We support programs from discovery through gram and multi-kilogram scale with CMC-aligned purification and QC planning.

Can you support custom linker and spacer design for peptide conjugates?

Yes. We support customizable linker and spacer architectures (e.g., PEG/TEG spacers, stable or cleavable linkers) to optimize steric accessibility, conjugation efficiency, and functional performance. Linker selection can be aligned with your mechanism of action, analytical requirements, and scale-up strategy.

More FAQ'sAll FAQ's

Recommended Reading

Selected references on peptide conjugation and oligonucleotide delivery concepts.

  1. Dowdy SF. Overcoming cellular barriers for RNA therapeutics. Nat Biotechnol. 2017;35:222–229.
  2. Järver P, Langel Ü. Cell-penetrating peptides—A brief introduction. Biochim Biophys Acta. 2006;1758:260–263.
  3. Juliano R. The delivery of therapeutic oligonucleotides. Nucleic Acids Res. 2016;44:6518–6548.

If you want, I can tailor this list to CPP–PMO/PNA literature and add inline citations to the page.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

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