Bio-Synthesis offers a complete array of fluorescent reporters and quenchers. Our product offering includes common dye modifiers such as 6-FAM, HEX, and TET, as well as exotic modifications. We can directly attach fluorescent dyes to oligos at the 5'-end, 3'-end, and even internal positions of an oligonucleotide during synthesis. When certain modifications (such as Digoxigenin and some fluorescent dyes) are not available for direct incorporation, cross-linking two molecules using a specific functional group, such as an amine, is utilized. Modifying an oligonucleotide with a thiol group and a specific cross-linker enables the linking of two oligonucleotides together. A post-synthetic chemical modification of an oligonucleotide results in lower yields than modifications introduced during synthesis. Furthermore, all post-synthetic conjugations require HPLC purification. Post-synthetic chemical modifications can result in decreased yields; therefore, we do not recommend PAGE purification.
All qPCR probes are available at a fixed price per oligonucleotide, within the following parameters:
- Synthesis of the custom oligos up to 30 bases in length
- Deprotected, desalted and HPLC purified (>85% pure)
- Wide range of dye and quencher options available
- QC by MALDI-TOP Mass Spectrometry, PAGE and Fluoroscan
- 3-10 working days turnaround, pending successful QC validation
- Assistance with the design is available, free of charge